Name: solid plate-based dietary restriction in caenorhabditis elegans
Uploaded: 2011-05-28T14:00:00
Description: Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Este trabajo fue apoyado por un premio de piloto del Centro de choque Nathan (P30 AG13283) y una subvención R01 (R01 AG028516) del Instituto Nacional sobre el Envejecimiento (NIA) para A.-LH

<html> 1. El establecimiento de una curva de calibración para la estimación de las concentraciones de bacterias En esta sección se describe un método para la estimación de la concentración de bacterias de cualquier cepa determinada bacteria (por ejemplo, OP50, HT115) que se utilizará como fuente de alimento para los experimentos de restricción dietética en C. elegans. Las curvas de calibración independientes deben ser establecidos para cada cepa de la bacteria utilizada. ...

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La restricción dietética (DR) se sabe que aumenta la vida útil y retrasar la aparición de diversas enfermedades relacionadas con la edad en una amplia gama de especies, incluyendo mamíferos 7.5. Manipulaciones ambientales y genéticos se han utilizado para el modelo DR en C. elegans, incluidos los gusanos de cultivo en un medio líquido semi-definido 8,9, la dilución de la fuente de alimento de bacterias en un medio líquido 10 a 12, y el uso de mutantes de comportamiento ...

<table border="1"><tbody><tr><td> Nombre del reactivo </td><td> Empresa </td><td> Número de catálogo </td><td> Comentarios </td></tr><tr><td> Carbenicilina </td><td> Duchefa Biochemie </td><td> C0109.25 </td><td> Disuelto en el agua. </td></tr><tr><td> Kanamicina </td><td> Roche </td><td> 10106801001 </td><td> Disuelto en el agua. </td></tr><tr><td> Bacto-peptona </td><td> Becton, Dickinson and Company </td><td> DF0118072 </td><td></td></tr><tr><td> Bacto-triptona </td><td> Becton, Dickinson and Company </td><td> DF0123075 </td><td></td></tr><tr><td> Bacto-extracto de levadura </td><td> Becton, Dickinson and Company </td><td> DF0127071 </td><td></td></tr><tr><td> Agar </td><td> Becton, Dickinson and Company </td><td> DF00145170 </td><td></td></tr><tr><td> Tubo de cultivo estéril </td><td> EE.UU. Científico </td><td> 1485-0810 </td><td></td></tr><tr><td> Espectrofotómetro </td><td> Amersham Biosciences </td><td> Gene Quant pro </td><td> Amersham ahora es parte de GE Healthcare. </td></tr><tr><td> Microscopio de disección </td><td> Zeiss </td><td> Stemi 2000 </td><td></td></tr><tr><td> Centrífugo </td><td> Beckman Coulter </td><td> Accuspin FR </td><td></td></tr></tbody></table>

solid plate-based dietary restriction in caenorhabditis elegans

Reducción de la ingesta de alimentos sin desnutrición o inanición se sabe que aumenta la vida útil y retrasar la aparición de diversas enfermedades relacionadas con la edad en una amplia gama de especies, incluyendo mamíferos. También provoca una disminución en el peso corporal y la fertilidad, así como niveles más bajos de glucosa en plasma, la insulina y el IGF-1 en estos animales. Este tratamiento se refiere a menudo como la restricción dietética (DR) o la restricción calórica (CR). El nematodo Caenorhabditis elegans se ha convertido en un importante organismo modelo para estudiar la biología del envejecimiento. Manipulaciones ambientales y genéticos se han utilizado para el modelo DR y han demostrado prolongar la vida de C. elegans. Sin embargo, muchos de los estudios reportados en la República Dominicana C. elegans se realizaron mediante la propagación de los animales en un medio líquido, mientras que la mayoría de los estudios de genética en el campo del envejecimiento se realiza en el estándar de agar sólido en placas de Petri. Aquí se presenta un protocolo estándar de DR con NGM agar sólido basado en la placa con bacterias muertas.

Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Aquí se presenta un protocolo para la realización de placa sólida basada en la restricción dietética en<em&gt; C. elegans</em&gt; Con bacterias muertas.

Placa Sólida basado en la restricción dietética Caenorhabditis elegans</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

Research

JoVE Journal

Biology

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

Medición Caenorhabditis elegans Duración de la vida en el medio sólido

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

Investigación

Biología

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

El aislamiento y la In vitro La activación de Caenorhabditis elegans El esperma

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse microscopía de embriogénesis temprana en Caenorhabditis elegans

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Básico<em&gt; Caenorhabditis elegans</em&gt; Métodos de sincronización y de observación

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Extracción y análisis de prostaglandina en<em&gt; Caenorhabditis elegans</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

La suplementación dietética de ácidos grasos poliinsaturados en<em&gt; Caenorhabditis elegans</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

El cultivo de<em&gt; Caenorhabditis elegans</em&gt; En tres dimensiones en el Laboratorio

The use of genetic model organisms such as Caenorhabditis elegans has led to seminal discoveries in biology over the last five decades. Most of what we ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Medición de las tasas de consumo de oxígeno en intacto Caenorhabditis elegans

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Cuantificación de la disminución proteostática específica del tejido en Caenorhabditis elegans

The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a ...

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modelar enfermedades neurodegenerativas asociadas a la edad en Caenorhabditis elegans

Battling human neurodegenerative pathologies and managing their pervasive socioeconomic impact is becoming a global priority. Notwithstanding their ...