Name: solid plate-based dietary restriction in caenorhabditis elegans
Uploaded: 2011-05-28T14:00:00
Description: Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Detta arbete stöddes av en pilot utmärkelse från Nathan Shock Center (P30 AG13283) och en R01 bidrag (R01 AG028516) från National Institute on Aging (NIA) till A.-LH

<html> 1. Upprättande av en kalibreringskurva för uppskattning av bakterier koncentrationer Detta avsnitt beskriver en metod för beräkning av bakterier koncentration för varje givet bakterietyp (t.ex. OP50, HT115) som kommer att användas som en näringskälla för dietrestriktioner experiment i C. elegans. Separata kalibreringskurvor bör fastställas för varje bakterier stam som använts. <ol><li> Inokulera en enda koloni av E. coli OP50 bakterier plockade från en...

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Dietrestriktioner (DR) är känd för att öka livslängd och fördröja uppkomsten av olika åldersrelaterade sjukdomar i ett brett spektrum av arter, inklusive däggdjur 5-7. Både miljömässiga och genetiska manipulationer har använts för att modellera DR i C. elegans, inklusive odling maskar i en semi-definierat flytande medium 8,9, utspädning av den bakteriella föda i flytande medium 10-12, och användning av beteenden mutanter som foder i en långsammare takt 13....

<table border="1"><tbody><tr><td> Reagensets namn </td><td> Företag </td><td> Katalognummer </td><td> Kommentarer </td></tr><tr><td> Carbenicillin </td><td> Duchefa Biochemie </td><td> C0109.25 </td><td> Upplöst i vatten. </td></tr><tr><td> Kanamycin </td><td> Roche </td><td> 10106801001 </td><td> Upplöst i vatten. </td></tr><tr><td> Bacto-pepton </td><td> Becton, Dickinson and Company </td><td> DF0118072 </td><td></td></tr><tr><td> Bacto-Tryptone </td><td> Becton, Dickinson and Company </td><td> DF0123075 </td><td></td></tr><tr><td> Bacto-jästextrakt </td><td> Becton, Dickinson and Company </td><td> DF0127071 </td><td></td></tr><tr><td> Agar </td><td> Becton, Dickinson and Company </td><td> DF00145170 </td><td></td></tr><tr><td> Sterila kultur röret </td><td> USA Vetenskapliga </td><td> 1485-0810 </td><td></td></tr><tr><td> Spektrofotometer </td><td> Amersham Biosciences </td><td> Gene Quant pro </td><td> Amersham är nu en del av GE Healthcare. </td></tr><tr><td> Analysera Mikroskop </td><td> Zeiss </td><td> STEMI 2000 </td><td></td></tr><tr><td> Centrifugera </td><td> Beckman Coulter </td><td> Accuspin FR </td><td></td></tr></tbody></table>

solid plate-based dietary restriction in caenorhabditis elegans

Minskning av födointag utan undernäring eller svält är kända för att öka livslängd och fördröja uppkomsten av olika åldersrelaterade sjukdomar i ett brett spektrum av arter, inklusive däggdjur. Det orsakar också en minskning i kroppsvikt och fertilitet, samt lägre nivåer av plasmaglukos, insulin och IGF-1 hos dessa djur. Denna behandling är ofta som dietrestriktioner (DR) eller kalorimängden (CR). Nematoden Caenorhabditis elegans har vuxit fram som en viktig organism i forskningen för att studera åldrandets biologi. Både miljömässiga och genetiska manipulationer har använts för att modellera DR och har visat sig förlänga livslängden i C. elegans. Men många av de rapporterade DR studier i C. elegans gjordes av förökningsmaterial djur i flytande medier, medan de flesta av de genetiska studier på den åldrande fältet gjordes på den standard fast agar i petri plattor. Här presenterar vi ett DR-protokoll med vanliga fasta NGM agar-baserad plåt med dödade bakterier.

Watch this Scientific Journal Video about Solid Plate-based Dietary Restriction in Caenorhabditis elegans at JoVE.com

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Här presenterar vi ett protokoll för att utföra Plattor-baserade dietrestriktioner i<em&gt; C. elegans</em&gt; Med dödade bakterier.

Solid Plate-baserade dietrestriktioner i Caenorhabditis elegans</em

Reduction of food intake without malnutrition or starvation is known to increase lifespan and delay the onset of various age-related diseases in a wide ...

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Biology

Solid Plate-based Dietary Restriction in Caenorhabditis elegans

Measuring Caenorhabditis elegans Life Span on Solid Media

Measuring Caenorhabditis elegans Life Span on Solid Media

Mätning Caenorhabditis elegans Livslängd på fasta medier

Aging is a degenerative process characterized by a progressive deterioration of cellular components and organelles resulting in mortality. The nematode ...

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolation and In vitro Activation of Caenorhabditis elegans Sperm

Isolering och In vitro Aktivering av Caenorhabditis elegans Spermier

Males and hermaphrodites are the two naturally found sexual forms in the nematode C. elegans. The amoeboid sperm are produced by both males and ...

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans

Time-lapse Mikroskopi av tidig embryogenesen i Caenorhabditis elegans

Caenorhabditis elegans has often been used as a model system in studies of early developmental processes.  The transparency of the 
embryos, the genetic ...

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basic Caenorhabditis elegans Methods: Synchronization and Observation

Basisk<em&gt; Caenorhabditis elegans</em&gt; Metoder: Synkronisering och miljöövervakning

Research into the molecular and developmental biology of the nematode Caenorhabditis elegans was begun in the early seventies by Sydney Brenner and it has ...

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraktion och analys i<em&gt; Caenorhabditis elegans</em

Caenorhabditis elegans is emerging as a powerful animal model to study the biology of lipids1-9. Prostaglandins are an important class of eicosanoids, ...

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Kosttillskott av fleromättade fettsyror i<em&gt; Caenorhabditis elegans</em

Fatty acids are essential for numerous cellular functions. They serve as efficient energy storage molecules, make up the hydrophobic core of membranes, ...

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

odling av<em&gt; Caenorhabditis elegans</em&gt; I tre dimensioner i laboratoriet

The use of genetic model organisms such as Caenorhabditis elegans has led to seminal discoveries in biology over the last five decades. Most of what we ...

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans

Mätning av syre förbrukning i intakt Caenorhabditis elegans

Optimal mitochondrial function is critical for healthy cellular activity, particularly in cells that have high energy demands like those in the nervous ...

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans

Kvantifiering av vävnadsspecifik proteostatisk nedgång i caenorhabditis elegans

The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a ...

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Modellering Åldersassocierade neurodegenerativa sjukdomar i Caenorhabditis elegans

Battling human neurodegenerative pathologies and managing their pervasive socioeconomic impact is becoming a global priority. Notwithstanding their ...