A Gradient-generating Microfluidic Device for Cell Biology

15.2K Views

11:05 min

August 30th, 2007

DOI :

10.3791/271-v

August 30th, 2007


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Gradient generating Microfluidic Device

Chapters in this video

0:05

Introduction

0:21

Microfabrication of microfluidic device

0:56

Preparation of PDMS mixture

1:49

Patterning PDMS molds

2:20

Removing bubbles by vacuum

3:19

Assembly of microfluidic device

3:20

Cutting layers of microfluidic device

3:33

Creating channel inlet and outlet

3:59

Bonding the microfluidic device layers

6:55

Experiment: effect of EGF gradient on cell migration

7:13

Application of reagents

9:49

Experiment: cell migration

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