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33.1K Views
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06:40 min
May 10th, 2011
DOI :
10.3791/2710-v
Chapters
0:05
Title
0:45
In Situ Perfusion of Mouse Livers with Digestive Enzymes
2:37
In Vitro Digestion of Mouse Livers
3:10
Density Gradient Centrifugation
5:29
Results of Isolating Hepatic Stellate Cells
6:09
Conclusion
Summary
在这里,我们描述了从小鼠肝的肝星状细胞的分离方法。小鼠肝脏星状细胞的纯化,消化原位在体外链霉蛋白酶,胶原酶治疗之前,密度梯度离心法。这种技术产生高纯度的肝星状细胞。
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