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15.2K Views
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08:38 min
May 25th, 2011
DOI :
10.3791/2752-v
Chapters
0:05
Title
2:14
Brain Slice Preparation
5:14
Electrophysiological Recording
6:39
Representative Results for Presynaptic Terminal Imaging and Synaptic Transmission Recording
7:51
Conclusion
3:22
Flame-Sealed Glass Micropipette Preparation and Vibrodissociation
Summary
这份报告表明保留个别可行的神经元连接的突触前boutons的机械隔离技术。 Vibrodissociated的神经元具有快速生产的优势,卓越的药理控制和改进的空间不影响邻近的细胞钳。这种方法可用于突触元素的成像和膜片钳记录。
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