Name: trichuris muris infection: a model of type 2 immunity and inflammation in the gut
Uploaded: 2011-05-24T12:00:00
Description: Watch this Scientific Journal Video about Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut at JoVE.com

この作品は、カナダ衛生研究所（MSH - 95368、MOP - 89773とCZのMOP - 106623）とイノベーションのためのカナダ財団によってサポートされていました。 SCMは、消化器ポスドクのCIHR /カナダの協会の受信者です。 CZはCIHR新しい研究者でもある。

1。伝播するネズミ鞭虫の卵<ol><li> （または遺伝的に感受性のあるマウスネズミ鞭虫の卵の新しいバッチを生成するには、20〜30免疫不全マウスに感染（）例：NOD.Cg - Prkdc SCID Il2rg tm1Wjl / SzJ（NSG）または129S6/SvEvTac-Rag2 tm1Fwa（RAG2 - - /）例：。経口胃管栄養法により約300ネズミ鞭虫の卵を6〜8週間経過しているAKR / J）。 </li><li> 32から35日後にCO 2</su...

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	<li>Cliffe, L. J., Grencis, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Trichuris+muris+system:+a+paradigm+of+resistance+and+susceptibility+to+intestinal+nematode+infection.">The Trichuris muris system: a paradigm of resistance and susceptibility to intestinal nematode infection.</a> Adv. Parasitol. 57, 255-307 (2004).</li><li>Else, K. J., Finkelman, F. D., Maliszewski, C. R., Grencis, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytokine-mediated+regulation+of+chronic+intestinal+helminth+infection.">Cytokine-mediated regulation of chronic intestinal helminth infection.</a> J. Exp. Med. 179, 347-351 (1994).</li><li>Bancroft, A. J., Grencis, R. 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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accelerated+intestinal+epithelial+cell+turnover:+a+new+mechanism+of+parasite+expulsion.">Accelerated intestinal epithelial cell turnover: a new mechanism of parasite expulsion.</a> Science. 308, 1463-1465 (2005).</li><li>Artis, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+weapons+in+the+war+on+worms:+identification+of+putative+mechanisms+of+immune-mediated+expulsion+of+gastrointestinal+nematodes.">New weapons in the war on worms: identification of putative mechanisms of immune-mediated expulsion of gastrointestinal nematodes.</a> Int. J. Parasitol. 36, 723-733 (2006).</li><li>Finkelman, F. 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このプロトコルは、詳細調査員の必要に応じて変更できます標準の高用量急性ネズミ鞭虫の感染を。例えば、マウスを屠殺することができ、組織は別の日に収穫。マウスが正常に完全なワームの負担が確立されていることを決定するために、それらはすべてマウスでは約200ワームの重荷を運ぶ必要のある時点で、14日目に犠牲にすることができます。マウスはまた、検出されたワーム?...

<table border="1"><tbody><tr><th>試薬の名前</th><th>会社</th><th>カタログ番号</th><th>コメント</th></tr><tr><td>動物給餌針（18 × 1 ½&quot;） </td><td>ポッパー</td><td> 7912 </td><td></td></tr><tr><td>滑らかな曲面鉗子</td><td> Roboz </td><td> RS - 5047 </td><td></td></tr><tr><td> DMEM </td><td>ギブコ</td><td> 11965 </td><td></td></tr><tr><td> NOD.Cg - Prkdc SCID Il2r gtm1Wjl / SzJ（NSG） </td><td>ジャクソン研究所</td><td> 005557 </td><td>これらは我々が使用したマウスは、しかし、いかなる免疫不全マウスや感受性株は動作するはずです。 </td></tr><tr><td> RNAlaterは</td><td>キアゲン</td><td> 76104 </td><td></td></tr><tr><td> 2 mlのチューブ</td><td> Axygen </td><td> MCT - 200 - C </td><td></td></tr><tr><td> 15 mlチューブ</td><td>ファルコン</td><td> 352096 </td><td></td></tr><tr><td> 6ウェルプレート</td><td>ファルコン</td><td> 353046 </td><td></td></tr><tr><td>パラホルムアルデヒド</td><td>電子顕微鏡の科学</td><td> 15710 </td><td></td></tr><tr><td> α-RELMβ抗体</td><td>ぺプロテック株式会社</td><td> 0694270Rb </td><td></td></tr></tbody></table>

trichuris muris infection: a model of type 2 immunity and inflammation in the gut

ネズミ鞭虫はネズミの天敵病原体であり、ヒトや家畜1を感染させる鞭の種の生物学的および抗原的に類似しています。感染卵を経口胃管栄養法、遠位小腸で孵化によって与えられる、環境1に線が盲腸の陰窩と近位結腸そのと成熟時に腸上皮細胞（IECS）ワームのリリースの卵に侵入する。このモデルは、コントロールのCD4 + Tヘルパー（Th）細胞の活性化だけでなく、腸上皮の変化その要因を調べるために強力なツールです。このようなC57BL / 6およびBALB / cのような耐近交系、で発生する免疫応答が、Th2の偏サイトカイン（IL - 4、IL - 5およびIL - 13）とTh1細胞関連サイトカイン間（ILワームの追放によって特徴付けられる-12、IL - 18、IFN -γ）遺伝的に感受性のAKR / Jマウス2-6の慢性感染を促進する。 Th2サイトカインが急速IECSの売上高、杯細胞の分化、募集およびワームの追放7-15に関与しているすべてが上皮透過性と平滑筋収縮の変化、を含む腸内微小環境における生理学的変化を促進する。ここでは詳細にその後の実験で使用することができるネズミ鞭虫の卵を伝播するためのプロトコルを。我々はまた、感染後の分析のための提案をサンプル実験的な収穫を提供しています。全体的に、このプロトコルは番目の消化管における傾向と同様にIECSの免疫エフェクター機能に関係する質問に対処するために使用することができるネズミ鞭虫のマウス感染モデルを実行するための基本的なツールを研究者に提供します。

Watch this Scientific Journal Video about Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut at JoVE.com

Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut

ネズミ鞭虫の感染は、抵抗性のマウスは、保護のTh2応答を生成し、影響を受けやすいマウスは、病理学的Th1応答を生成するTh2の免疫の腸のモデルです。

ネズミ鞭虫感染症：腸内のタイプ2免疫と炎症のモデル

Trichuris muris is a natural pathogen of mice and is biologically and antigenically similar to species of Trichuris that 
infect humans and livestock1. ...

Research

JoVE Journal

Immunology and Infection

Trichuris muris Infection: A Model of Type 2 Immunity and Inflammation in the Gut

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Although community-acquired pneumonia remains a major public health problem, murine models of bacterial pneumonia have recently facilitated significant ...

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