Name: detection of nitric oxide and superoxide radical anion by electron paramagnetic resonance spectroscopy from cells using spin traps
Uploaded: 2012-08-18T14:00:00
Description: Watch this Scientific Journal Video about Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps at JoVE.com

Dette arbeidet ble finansiert av NIH National Heart, Lung, and Blood Institute stipend RO1 HL81248.

1. Kultur av storfe aorta endotelceller (BAEC) <ol><li> Riktig aseptiske teknikker ble fulgt. </li><li> I et vannbad, varm medium uten antibiotika ved 37 ° C. </li></ol> Merk: medium består av fenol gratis Dulbecco modifiserte Eagle medium (DMEM) med 4,5 g / L D-glukose, 4 mm L-glutamin, 1% ikke-essensielle aminosyrer, supplert med 10% fosterets storfe serum (FBS) og 2,5 mg / L endotelial vekstfaktor. <ol start="3"><li> Fjern T75 flasken inneholder celler fra inkubatoren ...

<ol>
	<li>Winterbourn, C. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reconciling+the+chemistry+and+biology+of+reactive+oxygen+species.">Reconciling the chemistry and biology of reactive oxygen species.</a> Nat. Chem. Biol. 4, 278-286 (2008).</li><li>Winterbourn, C. C., Hampton, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thiol+chemistry+and+specificity+in+redox+signaling.">Thiol chemistry and specificity in redox signaling.</a> Free Radic. Biol. Med. 45, 549-561 (2008).</li><li>Clerch, L. B., Massaro, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Oxygen, Gene Expression, and Cellular Function. , (1997).</li><li>Gutteridge, J. M. C., Halliwell, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidants:+Molecules,+medicines,+and+myths.">Antioxidants: Molecules, medicines, and myths.</a> Biochem. Biophys. Res. Commun. 393, 561-564 (2010).</li><li>Sumimoto, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structure,+regulation+and+evolution+of+Nox-family+NADPH+oxidases+that+produce+reactive+oxygen+species.">Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species.</a> FEBS J. 275, 3249-3277 (2008).</li><li>Ignarro, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Editor Nitric Oxide: Biology and Pathobiology. , (2009).</li><li>Moreau, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+effects+of+alkyl-+and+arylguanidines+on+the+stability+and+reactivity+of+inducible+NOS+heme-dioxygen+complexes.">Differential effects of alkyl- and arylguanidines on the stability and reactivity of inducible NOS heme-dioxygen complexes.</a> Biochemistry. 45, 3988-3999 (2006).</li><li>Vasquez-Vivar, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Superoxide+generation+by+endothelial+nitric+oxide+synthase:+the+influence+of+cofactors.">Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors.</a> Proc. Natl. Acad. Sci. U. S. A. 95, 9220-9225 (1998).</li><li>Villamena, F. A., Zweier, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+reactive+oxygen+and+nitrogen+species+by+EPR+spin+trapping.">Detection of reactive oxygen and nitrogen species by EPR spin trapping.</a> Antioxid. Redox Signal. 6, 619-629 (2004).</li><li>Finkelstein, E., Rosen, G. M., Rauckman, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping+of+superoxide+and+hydroxyl+radical:+practical+aspects.">Spin trapping of superoxide and hydroxyl radical: practical aspects.</a> Arch. Biochem. Biophys. 200, 1-16 (1980).</li><li>Frejaville, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=5-Diethoxyphosphoryl-5-methyl-1-pyrroline+N-oxide+(DEPMPO):+a+new+phosphorylated+nitrone+for+the+efficient+in+vitro+and+in+vivo+spin+trapping+of+oxygen-centered+radicals.">5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO): a new phosphorylated nitrone for the efficient in vitro and in vivo spin trapping of oxygen-centered radicals.</a> J. Chem. Soc., Chem. Commun. , 1793-1794 (1994).</li><li>Olive, G., Mercier, A., Le Moigne, F., Rockenbauer, A., Tordo, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=2-Ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide:+Evaluation+of+the+spin+trapping+properties.">2-Ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: Evaluation of the spin trapping properties.</a> Free Radical. Biol. Med. 28, 403-408 (2000).</li><li>Villamena, F. A., Zweier, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Superoxide+radical+trapping+and+spin+adduct+decay+of+5-tert-butoxycarbonyl-5-methyl-1-pyrroline+N-oxide+(BocMPO):+kinetics+and+theoretical+analysis.">Superoxide radical trapping and spin adduct decay of 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BocMPO): kinetics and theoretical analysis.</a> J. Chem. Soc., Perkin Trans. 2, 1340-1344 (2002).</li><li>Tsuchiya, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nitric+oxide-forming+reactions+of+the+water-soluble+nitric+oxide+spin-trapping+agent,+MGD.">Nitric oxide-forming reactions of the water-soluble nitric oxide spin-trapping agent, MGD.</a> Free Radical Biol. Med. 27, 347-355 (1999).</li><li>Vanin, A. F., Poltorakov, A. P., Mikoyan, V. D., Kubrina, L. N., van Faassen, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Why+iron-dithiocarbamates+ensure+detection+of+nitric+oxide+in+cells+and+tissues.">Why iron-dithiocarbamates ensure detection of nitric oxide in cells and tissues.</a> Nitric Oxide. 15, 295-311 (2006).</li><li>RojasWahl, R. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decomposition+mechanism+of+3-N-morpholinosydnonimine+(SIN-1):+A+density+functional+study+on+intrinsic+structures+and+reactivities.">Decomposition mechanism of 3-N-morpholinosydnonimine (SIN-1): A density functional study on intrinsic structures and reactivities.</a> J. Mol. Model. 10, 121-129 (2004).</li><li>Klempner, M. S., Gallin, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separation+and+functional+characterization+of+human+neutrophil+subpopulations.">Separation and functional characterization of human neutrophil subpopulations.</a> Blood. 51, 659-669 (1978).</li><li>Pou, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping+of+nitric+oxide+by+ferro-chelates:+kinetic+and+in+vivo+pharmacokinetic+studies.">Spin trapping of nitric oxide by ferro-chelates: kinetic and in vivo pharmacokinetic studies.</a> Biochim. Biophys. Acta. 1427, 216-226 (1999).</li><li>Finkelstein, E., Rosen, G. M., Rauckman, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping.+Kinetics+of+the+reaction+of+superoxide+and+hydroxyl+radicals+with+nitrones.">Spin trapping. Kinetics of the reaction of superoxide and hydroxyl radicals with nitrones.</a> J. Am. Chem. Soc. 102, 4994-4999 (1980).</li><li>Britigan, B. E., Rosen, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin-trapping+and+human+neutrophils.+Limits+of+detection+of+hydroxyl+radical.">Spin-trapping and human neutrophils. Limits of detection of hydroxyl radical.</a> J. Biol. Chem. 264, 12299-12302 (1989).</li><li>Frejaville, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline+N-oxide:+A+new+efficient+phosphorylated+nitrone+for+the+in+vitro+and+in+vivo+spin+trapping+of+oxygen-centered+radicals.">5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: A new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals.</a> J. Med. Chem. 38, 258-265 (1995).</li><li>Snyrychova, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improvement+of+the+sensitivity+of+EPR+spin+trapping+in+biological+systems+by+cyclodextrins:+A+model+study+with+thylakoids+and+photosystem+II+particles.">Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles.</a> Free Radical Biol. Med. 48, 264-274 (2010).</li><li>Han, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipophilic+beta-cyclodextrin+cyclic-nitrone+conjugate:+Synthesis+and+spin+trapping+studies.">Lipophilic beta-cyclodextrin cyclic-nitrone conjugate: Synthesis and spin trapping studies.</a> J. Org. Chem. 74, 5369-5380 (2009).</li><li>Han, Y., Tuccio, B., Lauricella, R., Villamena, F. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+spin+trapping+properties+by+beta-cyclodextrin-cyclic+nitrone+conjugate.">Improved spin trapping properties by beta-cyclodextrin-cyclic nitrone conjugate.</a> J. Org. Chem. 73, 7108-7117 (2008).</li><li>Hardy, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection,+characterization,+and+decay+kinetics+of+ROS+and+thiyl+adducts+of+mito-DEPMPO+spin+trap.">Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap.</a> Chem. Res. Toxicol. 20, 1053-1060 (2007).</li><li>Kim, S. -. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+reactivity+of+a+cyclic+nitrone-calix[4]pyrrole+conjugate+with+superoxide+radical+anion:+Theoretical+and+experimental+studies.">Fast reactivity of a cyclic nitrone-calix[4]pyrrole conjugate with superoxide radical anion: Theoretical and experimental studies.</a> J. Am. Chem. Soc. 132, 17157-17173 (2010).</li></ol>

EPJ spin fangst har vært ansatt i et bredt spekter av biomedisinske applikasjoner for å kvantifisere og identifisere frie radikaler. Spin fangst er svært følsom, i stand til å oppdage radikaler i konsentrasjoner som varierte fra nM til mM dermed gjør det egnet for bruk i biologiske systemer. Dannelsen av paramagnetiske addukt, NO-Fe 2 +-MGD, er grunnlaget for NO deteksjon via EPJ. Fe 2 +-MGD reagerer med NO raskt 18 til en rate på ~ 10 6 M -1 s ...

<table border="1"><tbody><tr><td> Navn på reagensen </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer (valgfritt) </td></tr><tr><td> Fenol gratis DMEM medium Høy glukose 1X </td><td> GIBCO </td><td> 31053 </td><td></td></tr><tr><td> 0,25% Trypsin-EDTA </td><td> GIBCO </td><td> 25200 </td><td></td></tr><tr><td> L-Glutamin </td><td> Fisher Scientific </td><td> BP379-100 </td><td></td></tr><tr><td> MEM ikke livsnødvendige aminosyrer </td><td> GIBCO </td><td> 11140 </td><td></td></tr><tr><td> Fetal bovin serum </td><td> Atlanta Biologicals </td><td> S11550 </td><td></td></tr><tr><td> Endotelial vekstfaktor </td><td> Millipore </td><td> 02-102 </td><td></td></tr><tr><td> CAI </td><td> Enzo og biovitenskap </td><td> A-23187 </td><td> Løs opp i DMSO </td></tr><tr><td> SIN-1 </td><td> Enzo og biovitenskap </td><td> BML-CN245-0020 </td><td></td></tr><tr><td> DMPO </td><td> Dojindo Laboratories </td><td> D048-10 </td><td></td></tr><tr><td> FeSO 4 0.7 H 2 O </td><td> Sigma Aldrich </td><td> 215 422-250G </td><td> Løs opp i PBS med Ca og Mg </td></tr><tr><td> MGD </td><td> Enzo og biovitenskap </td><td> ALX-400-014-M050 </td><td> Løs opp i PBS med Ca 2 + og Mg 2 + </td></tr><tr><td> BAEC celler </td><td> Cell Systems </td><td> 2B2-C75 </td><td></td></tr><tr><td> DMSO </td><td> Fisher Scientific </td><td> BP231-100 </td><td></td></tr><tr><td> DPBS </td><td> Sigma Aldrich </td><td> D8537 </td><td></td></tr><tr><td> DPBS med CaCl 2 og MgCl 2 </td><td> Sigma Aldrich </td><td> D8662 </td><td></td></tr><tr><td> Phorbol-myristate acetat (PMA) </td><td> Sigma Aldrich </td><td> 79346-1 mg </td><td></td></tr></tbody></table>

detection of nitric oxide and superoxide radical anion by electron paramagnetic resonance spectroscopy from cells using spin traps

Reaktivt nitrogen / oksygen arter (ROS / RNS) ved lave konsentrasjoner spille en viktig rolle i å regulere celle funksjon, signalering, og immunrespons, men i uregulerte konsentrasjoner er skadelig for celleviabilitet 1, 2. Mens levende systemer har utviklet seg med endogene og kosttilskudd antioksidant forsvarsmekanismer for å regulere ROS generasjon, er ROS produsert fortløpende som naturlig biprodukter av normal metabolisme av oksygen og kan forårsake oksidativ skade på biomolekyler som resulterer i tap av protein funksjon, DNA kløv, eller lipid peroxidation tre, og til slutt til oksidativt stress fører til celle skade eller død 4. Superoxide radikal anion (O 2 • -) er den viktigste forløper for noen av de mest oksiderende arter som er kjent for å eksistere i biologiske systemer som peroxynitrite og hydroksyl radikaler. Den generasjonen av O 2 • - signaliserer første tegn på oksidativt burst, og derfor jegts deteksjon og / eller deponering i biologiske systemer er viktig. I denne demonstrasjonen, O 2 • - ble generert fra polymorfnukleære nøytrofile (PMNs). Gjennom kjemotaktisk stimulering med phorbol-12-myristate-13-acetat (PMA), genererer PMN O 2 • - via aktivering av nikotinamid adenindinukleotid fosfat (NADPH) oksidase 5. Nitrogenoksid (NO) syntase som kommer i tre isoformer, som induserbar-, nerve-og endotelial-NOS, eller iNOS, nNOS eller Enos, henholdsvis katalyserer omdanning av L-arginin til L-citrulline, bruker NADPH å produsere NO 6 . Her genereres vi NO fra endotelceller. Under oksidativt stress forhold, kan Enos for eksempel bytte fra produsere NEI til O 2 • - i en prosess som kalles frakopling, som antas å være forårsaket av oksidasjon av heme 7 eller co-faktor, tetrahydrobiopterin (BH 4) 8. Det er bare fåpålitelige metoder for påvisning av frie radikaler i biologiske systemer, men er begrenset av spesifisitet og sensitivitet. Spin fangst blir ofte brukt for identifisering av frie radikaler og innebærer tillegg reaksjon på en radikal til en spin felle danner en vedvarende spin addukt som kan påvises ved elektronmikroskopi paramagnetisk resonans (EPR) spektroskopi. De ulike radikale addukter viser karakteristiske spekteret som kan brukes til å identifisere de radikale som skapes, og kan gi et vell av informasjon om natur og kinetikk av radikal produksjon ni. De sykliske nitrones og 5,5-dimethyl-pyrroline-N-oksid, 10 DMPO, den phosphoryl-substituerte DEPMPO 11, og ester-substituert, EMPO 12 og BMPO 13, har vært mye brukt som spin traps - sistnevnte spinn feller stille lenger halveringstid for O 2 • - addukt. Iron (II)-N-metyl-D-glucamine dithiocarbamate, Fe (MGD) 2 &lt;/ Sub&gt; er vanlig å felle NO grunn av høy forekomst av addukt formasjon og den høye stabiliteten i spinn addukt 14.

Watch this Scientific Journal Video about Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps at JoVE.com

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Electron paramagnetisk resonans (EPR) spektroskopi ble ansatt for å oppdage nitrogenoksid fra storfe aorta endotelceller og superoxide radikal anion fra menneskelige nøytrofile med jern (II)-N-metyl-D-glucamine dithiocarbamate, Fe (MGD)<sub&gt; 2</sub&gt; Og 5,5-dimethyl-1-pyroroline-N-oksid, DMPO, henholdsvis.

Påvisning av nitrogenoksid og Superoxide Radical anion med Electron paramagnetisk resonans spektroskopi fra celler ved hjelp av Spin Traps

Reactive nitrogen/oxygen species (ROS/RNS) at low concentrations play an important role in regulating cell function, signaling, and immune response but in ...

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