Name: detection of nitric oxide and superoxide radical anion by electron paramagnetic resonance spectroscopy from cells using spin traps
Uploaded: 2012-08-18T14:00:00
Description: Watch this Scientific Journal Video about Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps at JoVE.com

Detta arbete har finansierats av NIH National Heart, Lung, and Blood Institute bidrag RO1 HL81248.

1. Kultur av bovina aortaendotelceller (BAEC) <ol><li> Riktiga aseptisk teknik följdes. </li><li> I ett vattenbad, varmt medium utan antibiotika vid 37 ° C. </li></ol> Anmärkning: Det medium som består av fenol Dulbeccos modifierade Eagles medium (DMEM) med 4,5 g / L D-glukos, 4 mM L-glutamin, 1% icke-essentiella aminosyror, kompletterat med 10% fetalt bovint serum (FBS) och 2,5 mg / L endotelial tillväxtfaktor. <ol start="3"><li> Ta bort T75 kolv som innehåller celle...

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	<li>Winterbourn, C. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reconciling+the+chemistry+and+biology+of+reactive+oxygen+species.">Reconciling the chemistry and biology of reactive oxygen species.</a> Nat. Chem. Biol. 4, 278-286 (2008).</li><li>Winterbourn, C. C., Hampton, M. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thiol+chemistry+and+specificity+in+redox+signaling.">Thiol chemistry and specificity in redox signaling.</a> Free Radic. Biol. Med. 45, 549-561 (2008).</li><li>Clerch, L. B., Massaro, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Oxygen, Gene Expression, and Cellular Function. , (1997).</li><li>Gutteridge, J. M. C., Halliwell, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antioxidants:+Molecules,+medicines,+and+myths.">Antioxidants: Molecules, medicines, and myths.</a> Biochem. Biophys. Res. Commun. 393, 561-564 (2010).</li><li>Sumimoto, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structure,+regulation+and+evolution+of+Nox-family+NADPH+oxidases+that+produce+reactive+oxygen+species.">Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species.</a> FEBS J. 275, 3249-3277 (2008).</li><li>Ignarro, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Editor Nitric Oxide: Biology and Pathobiology. , (2009).</li><li>Moreau, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+effects+of+alkyl-+and+arylguanidines+on+the+stability+and+reactivity+of+inducible+NOS+heme-dioxygen+complexes.">Differential effects of alkyl- and arylguanidines on the stability and reactivity of inducible NOS heme-dioxygen complexes.</a> Biochemistry. 45, 3988-3999 (2006).</li><li>Vasquez-Vivar, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Superoxide+generation+by+endothelial+nitric+oxide+synthase:+the+influence+of+cofactors.">Superoxide generation by endothelial nitric oxide synthase: the influence of cofactors.</a> Proc. Natl. Acad. Sci. U. S. A. 95, 9220-9225 (1998).</li><li>Villamena, F. A., Zweier, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+reactive+oxygen+and+nitrogen+species+by+EPR+spin+trapping.">Detection of reactive oxygen and nitrogen species by EPR spin trapping.</a> Antioxid. Redox Signal. 6, 619-629 (2004).</li><li>Finkelstein, E., Rosen, G. M., Rauckman, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping+of+superoxide+and+hydroxyl+radical:+practical+aspects.">Spin trapping of superoxide and hydroxyl radical: practical aspects.</a> Arch. Biochem. Biophys. 200, 1-16 (1980).</li><li>Frejaville, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=5-Diethoxyphosphoryl-5-methyl-1-pyrroline+N-oxide+(DEPMPO):+a+new+phosphorylated+nitrone+for+the+efficient+in+vitro+and+in+vivo+spin+trapping+of+oxygen-centered+radicals.">5-Diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO): a new phosphorylated nitrone for the efficient in vitro and in vivo spin trapping of oxygen-centered radicals.</a> J. Chem. Soc., Chem. Commun. , 1793-1794 (1994).</li><li>Olive, G., Mercier, A., Le Moigne, F., Rockenbauer, A., Tordo, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=2-Ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide:+Evaluation+of+the+spin+trapping+properties.">2-Ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrrole-1-oxide: Evaluation of the spin trapping properties.</a> Free Radical. Biol. Med. 28, 403-408 (2000).</li><li>Villamena, F. A., Zweier, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Superoxide+radical+trapping+and+spin+adduct+decay+of+5-tert-butoxycarbonyl-5-methyl-1-pyrroline+N-oxide+(BocMPO):+kinetics+and+theoretical+analysis.">Superoxide radical trapping and spin adduct decay of 5-tert-butoxycarbonyl-5-methyl-1-pyrroline N-oxide (BocMPO): kinetics and theoretical analysis.</a> J. Chem. Soc., Perkin Trans. 2, 1340-1344 (2002).</li><li>Tsuchiya, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nitric+oxide-forming+reactions+of+the+water-soluble+nitric+oxide+spin-trapping+agent,+MGD.">Nitric oxide-forming reactions of the water-soluble nitric oxide spin-trapping agent, MGD.</a> Free Radical Biol. Med. 27, 347-355 (1999).</li><li>Vanin, A. F., Poltorakov, A. P., Mikoyan, V. D., Kubrina, L. N., van Faassen, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Why+iron-dithiocarbamates+ensure+detection+of+nitric+oxide+in+cells+and+tissues.">Why iron-dithiocarbamates ensure detection of nitric oxide in cells and tissues.</a> Nitric Oxide. 15, 295-311 (2006).</li><li>RojasWahl, R. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decomposition+mechanism+of+3-N-morpholinosydnonimine+(SIN-1):+A+density+functional+study+on+intrinsic+structures+and+reactivities.">Decomposition mechanism of 3-N-morpholinosydnonimine (SIN-1): A density functional study on intrinsic structures and reactivities.</a> J. Mol. Model. 10, 121-129 (2004).</li><li>Klempner, M. S., Gallin, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Separation+and+functional+characterization+of+human+neutrophil+subpopulations.">Separation and functional characterization of human neutrophil subpopulations.</a> Blood. 51, 659-669 (1978).</li><li>Pou, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping+of+nitric+oxide+by+ferro-chelates:+kinetic+and+in+vivo+pharmacokinetic+studies.">Spin trapping of nitric oxide by ferro-chelates: kinetic and in vivo pharmacokinetic studies.</a> Biochim. Biophys. Acta. 1427, 216-226 (1999).</li><li>Finkelstein, E., Rosen, G. M., Rauckman, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin+trapping.+Kinetics+of+the+reaction+of+superoxide+and+hydroxyl+radicals+with+nitrones.">Spin trapping. Kinetics of the reaction of superoxide and hydroxyl radicals with nitrones.</a> J. Am. Chem. Soc. 102, 4994-4999 (1980).</li><li>Britigan, B. E., Rosen, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spin-trapping+and+human+neutrophils.+Limits+of+detection+of+hydroxyl+radical.">Spin-trapping and human neutrophils. Limits of detection of hydroxyl radical.</a> J. Biol. Chem. 264, 12299-12302 (1989).</li><li>Frejaville, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline+N-oxide:+A+new+efficient+phosphorylated+nitrone+for+the+in+vitro+and+in+vivo+spin+trapping+of+oxygen-centered+radicals.">5-(Diethoxyphosphoryl)-5-methyl-1-pyrroline N-oxide: A new efficient phosphorylated nitrone for the in vitro and in vivo spin trapping of oxygen-centered radicals.</a> J. Med. Chem. 38, 258-265 (1995).</li><li>Snyrychova, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improvement+of+the+sensitivity+of+EPR+spin+trapping+in+biological+systems+by+cyclodextrins:+A+model+study+with+thylakoids+and+photosystem+II+particles.">Improvement of the sensitivity of EPR spin trapping in biological systems by cyclodextrins: A model study with thylakoids and photosystem II particles.</a> Free Radical Biol. Med. 48, 264-274 (2010).</li><li>Han, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lipophilic+beta-cyclodextrin+cyclic-nitrone+conjugate:+Synthesis+and+spin+trapping+studies.">Lipophilic beta-cyclodextrin cyclic-nitrone conjugate: Synthesis and spin trapping studies.</a> J. Org. Chem. 74, 5369-5380 (2009).</li><li>Han, Y., Tuccio, B., Lauricella, R., Villamena, F. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Improved+spin+trapping+properties+by+beta-cyclodextrin-cyclic+nitrone+conjugate.">Improved spin trapping properties by beta-cyclodextrin-cyclic nitrone conjugate.</a> J. Org. Chem. 73, 7108-7117 (2008).</li><li>Hardy, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection,+characterization,+and+decay+kinetics+of+ROS+and+thiyl+adducts+of+mito-DEPMPO+spin+trap.">Detection, characterization, and decay kinetics of ROS and thiyl adducts of mito-DEPMPO spin trap.</a> Chem. Res. Toxicol. 20, 1053-1060 (2007).</li><li>Kim, S. -. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+reactivity+of+a+cyclic+nitrone-calix[4]pyrrole+conjugate+with+superoxide+radical+anion:+Theoretical+and+experimental+studies.">Fast reactivity of a cyclic nitrone-calix[4]pyrrole conjugate with superoxide radical anion: Theoretical and experimental studies.</a> J. Am. Chem. Soc. 132, 17157-17173 (2010).</li></ol>

EPR spin trapping har varit anställd i ett brett spektrum av biomedicinska tillämpningar för att kvantifiera och identifiera fria radikaler. Spin trapping-är mycket känslig, kan detektera radikaler vid koncentrationer varierande från nM till | iM, vilket sålunda gör den lämplig för användning i biologiska system. Bildningen av det paramagnetiska addukt, NR-Fe2 +-MGD, är grunden för NO-detektion via EPR. Fe 2 +-MGD reagerar med NO snabbt 18 med en hastighet av ~ 10 <...

<table border="1"><tbody><tr><td> Namnet på reagens </td><td> Företaget </td><td> Katalognummer </td><td> Kommentarer (valfri) </td></tr><tr><td> Fenol DMEM-medium Hög glukos 1X </td><td> GIBCO </td><td> 31.053 </td><td></td></tr><tr><td> 0,25% Trypsin-EDTA </td><td> GIBCO </td><td> 25.200 </td><td></td></tr><tr><td> L-glutamin </td><td> Fisher Scientific </td><td> BP379-100 </td><td></td></tr><tr><td> MEM icke-essentiella aminosyror </td><td> GIBCO </td><td> 11.140 </td><td></td></tr><tr><td> Fetalt bovint serum </td><td> Atlanta Biologicals </td><td> S11550 </td><td></td></tr><tr><td> Endoteltillväxtfaktor </td><td> Millipore </td><td> 02-102 </td><td></td></tr><tr><td> CAI </td><td> Enzo Life Sciences </td><td> A-23187 </td><td> Lös i DMSO </td></tr><tr><td> SIN-1 </td><td> Enzo Life Sciences </td><td> BML-CN245-0020 </td><td></td></tr><tr><td> DMPO </td><td> Dojindo Laboratories </td><td> D048-10 </td><td></td></tr><tr><td> FeSO 4 0,7 H 2 O </td><td> Sigma Aldrich </td><td> 215.422-250G </td><td> Lös upp i PBS med Ca och Mg </td></tr><tr><td> MGD </td><td> Enzo Life Sciences </td><td> ALX-400-014-M050 </td><td> Lös upp i PBS med Ca 2 + och Mg 2 + </td></tr><tr><td> BAEC-celler </td><td> Cellsystem </td><td> 2B2-C75 </td><td></td></tr><tr><td> DMSO </td><td> Fisher Scientific </td><td> BP231-100 </td><td></td></tr><tr><td> DPBS </td><td> Sigma Aldrich </td><td> D8537 </td><td></td></tr><tr><td> DPBS med CaCl2 och MgCl2- </td><td> Sigma Aldrich </td><td> D8662 </td><td></td></tr><tr><td> Forbol-myristat-acetat (PMA) </td><td> Sigma Aldrich </td><td> 79.346-1 mg </td><td></td></tr></tbody></table>

detection of nitric oxide and superoxide radical anion by electron paramagnetic resonance spectroscopy from cells using spin traps

Reaktivt kväve / syrespecies (ROS / RNS) vid låga koncentrationer spela en viktig roll vid reglering av cellfunktioner, signalering, och immunsvar men med oreglerade koncentrationer är skadliga för cellviabilitet 1, 2. Även levande system har utvecklats med endogena och kostvanor mekanismer antioxidant försvar för att reglera ROS generation, är ROS produceras kontinuerligt som naturliga biprodukter av normal metabolism av syre och kan orsaka oxidativ skada på biomolekyler som resulterar i förlust av proteiners funktion, DNA klyvning, eller lipid peroxidation 3 och slutligen till oxidativ stress som leder till cellskada eller död 4. Superoxidradikalen anjon (O 2 • -) är den huvudsakliga prekursor av några av de mest höggradigt oxiderande arter som är kända för att existera i biologiska system, såsom peroxinitrit och hydroxylradikal. Generering av O 2 • - signalerar det första tecknet på oxidativt utbrott, och därför its detektering och / eller beläggs i biologiska system är viktig. I denna demonstration O 2 • - genererades från polymorfonukleära neutrofiler (PMN: er). Genom kemotaktisk stimulering med forbol-12-myristat-13-acetat (PMA), genererar PMN O 2 • - via aktivering av nikotinamid-adenin-dinukleotidfosfat (NADPH)-oxidas 5. Kväveoxid (NO)-syntas som finns i tre isoformer, som inducerbar-, neuronal-och endotel-NOS, eller iNOS, nNOS och eNOS, respektive, katalyserar omvandlingen av L-arginin till L-citrullin, med användning av NADPH som producerar NO 6 . Här genererade vi NO från endotelceller. Vid oxidativa stressförhållanden, kan eNOS exempelvis gå från att producera NO till O 2 • - i en process som kallas losskopplingen, som tros vara orsakad av oxidation av heme 7 eller co-faktor, tetrahydrobiopterin (BH4) 8. Det är endast ett fåtaltillförlitliga metoder för detektering av fria radikaler i biologiska system, men är begränsade av specificitet och känslighet. Spin trapping-är vanligen används för identifiering av fria radikaler och innefattar tillsats reaktion av en radikal med en spin trap bildar en ihållande spinn-addukt som kan detekteras genom elektronparamagnetisk resonans (EPR)-spektroskopi. De olika radikala addukterna uppvisar distinkta spektrum som kan användas för att identifiera radikalerna som genereras och kan ge en mängd information om natur och kinetik av radikal produktions 9. De cykliska nitroner, 5,5-dimetyl-pyrrolin-N-oxid, DMPO 10, den fosforyl-substituerade DEPMPO 11, och den ester-substituerad, EMPO 12 och BMPO 13, har använts i stor utsträckning som spin traps - sistnämnda spinn fällor uppvisar längre halveringstider för O 2 • - addukt. Järn (II)-N-metyl-D-glukamin ditiokarbamat, Fe (MGD) 2 &lt;/ Sub&gt; används ofta för att fånga NO på grund av höga adduktbildning och den höga stabiliteten i spinn addukten 14.

Watch this Scientific Journal Video about Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps at JoVE.com

Detection of Nitric Oxide and Superoxide Radical Anion by Electron Paramagnetic Resonance Spectroscopy from Cells using Spin Traps

Elektronparamagnetisk resonans (EPR)-spektroskopi användes för att detektera kväveoxid från bovina aortaendotelceller och superoxidradikalanjonen från humana neutrofiler med användning av järn (II)-N-metyl-D-glukamin ditiokarbamat, Fe (MGD)<sub&gt; 2</sub&gt; Och 5,5-dimetyl-1-pyroroline-N-oxid, DMPO, respektive.

Detektering av kväveoxid och superoxidradikalanjonen genom elektronparamagnetisk resonans-spektroskopi från celler med användning av spin traps

Reactive nitrogen/oxygen species (ROS/RNS) at low concentrations play an important role in regulating cell function, signaling, and immune response but in ...

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