Name: single drosophila ommatidium dissection and imaging
Uploaded: 2011-08-19T12:00:00
Description: Watch this Scientific Journal Video about Single Drosophila Ommatidium Dissection and Imaging at JoVE.com

我们感谢伯纳德沙鲁讨论。这项工作是由KCL的生物医学学院。

1。 果蝇视网膜的解剖<ol><li>填写一个3井载玻片，用磷酸盐缓冲液（PBS 1X），然后麻醉苍蝇的CO 2 。男性和女性都可以用于本次实验。 </li><li>苍蝇是麻醉后，拿起一个单一的飞行，使用镊子放入小碟的PBS。 </li><li>根据解剖范围，捏钳的长鼻，然后从身体分离的头撞断了脖子，使用产钳的第二套。 </li><li>控股的长鼻苍蝇头，沿左/右中线microscissors揭示大脑的飞头减少了一半。 </li><l...

     

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	<li>Marsh, J. L., Thompson, L. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drosophila+in+the+study+of+neurodegenerative+disease.">Drosophila in the study of neurodegenerative disease.</a> Neuron. 52, 169-178 (2006).</li><li>Mizushima, N., Levine, B., Cuervo, A. M., Klionsky, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophagy+fights+disease+through+cellular+self-digestion.">Autophagy fights disease through cellular self-digestion.</a> Nature. 451, 1069-1075 (2008).</li><li>Nisoli, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neurodegeneration+by+polyglutamine+Atrophin+is+not+rescued+by+induction+of+autophagy.">Neurodegeneration by polyglutamine Atrophin is not rescued by induction of autophagy.</a> Cell Death Differ. 17, 1577-1587 (2010).</li><li>Charroux, B., Fanto, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+fine+line+between+waste+disposal+and+recycling:+DRPLA+fly+models+illustrate+the+importance+of+completing+the+autophagy+cycle+for+rescuing+neurodegeneration.">The fine line between waste disposal and recycling: DRPLA fly models illustrate the importance of completing the autophagy cycle for rescuing neurodegeneration.</a> Autophagy. 6, 667-669 (2010).</li><li>Hardie, R. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voltage-sensitive+potassium+channels+in+Drosophila+photoreceptors.">Voltage-sensitive potassium channels in Drosophila photoreceptors.</a> J Neurosci. 11, 3079-3095 (1991).</li><li>Lee, T., Luo, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mosaic+analysis+with+a+repressible+cell+marker+(MARCM)+for+Drosophila+neural+development.">Mosaic analysis with a repressible cell marker (MARCM) for Drosophila neural development.</a> Trends in neurosciences. 24, 251-254 (2001).</li><li>Xu, T., Rubin, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+genetic+mosaics+in+developing+and+adult+Drosophila+tissues.">Analysis of genetic mosaics in developing and adult Drosophila tissues.</a> Development. 117, 1223-1237 (1993).</li><li>Gambis, A., Dourlen, P., Steller, H., Mollereau, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-color+in+vivo+imaging+of+photoreceptor+apoptosis+and+development+in+Drosophila.">Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila.</a> Dev Biol. 351, 128-1234 (2011).</li><li>Napoletano, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Polyglutamine+Atrophin+provokes+neurodegeneration+in+Drosophila+by+repressing+fat.">Polyglutamine Atrophin provokes neurodegeneration in Drosophila by repressing fat.</a> The EMBO journal. 30, 945-958 (2011).</li><li>Ravikumar, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inhibition+of+mTOR+induces+autophagy+and+reduces+toxicity+of+polyglutamine+expansions+in+fly+and+mouse+models+of+Huntington+disease.">Inhibition of mTOR induces autophagy and reduces toxicity of polyglutamine expansions in fly and mouse models of Huntington disease.</a> Nature. 36, 585-595 (2004).</li><li>Zou, S., Meadows, S., Sharp, L., Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genome-wide+study+of+aging+and+oxidative+stress+response+in+Drosophila+melanogaster.">Genome-wide study of aging and oxidative stress response in Drosophila melanogaster.</a> Proceedings of the National Academy of Sciences of the United States of America. 97, 13726-13731 (2000).</li></ol>

这里介绍的单ommatidium夹层使更深细胞像神经退行性疾病的进程， 在果蝇的眼睛为蓝本时的生物信息的收集。感光神经细胞更容易比其他神经元的访问和他们细胞质是非常相同的细胞在许多模型， 熟练使用量化体内的神经退行性疾病，特别大，因而适用于研究囊泡动力学。剥离的一个重要方面是主要的能力来执行它不固定的组织，绝不能暴露在空气中或干。这一基本的技术扩张的可?...

<table border="1"><tbody><tr><th>试剂名称</th><th>公司</th><th>目录编号</th><th>评论（可选） </th></tr><tr><td>便携式二氧化碳饮水机</td><td> Flystuff </td><td> 59-150 </td><td>笔芯也可用</td></tr><tr><td> DUMONT镊子5号</td><td>琼脂科学</td><td> T5034 </td><td></td></tr><tr><td> 3井载玻片</td><td>环境管理体系</td><td> 71561-01 </td><td></td></tr><tr><td>微型剪刀</td><td> VWR </td><td> HAMMHSB516 - 09 </td><td></td></tr><tr><td>施耐德公司的培养基</td><td> VWR </td><td> 733-1663 </td><td></td></tr><tr><td> LysoTracker红DND - 99 </td><td> Invitrogen公司</td><td> L7528 </td><td></td></tr><tr><td> Superfrost幻灯片</td><td> VWR </td><td> 631-0108 </td><td></td></tr><tr><td> Vectashield用DAPI </td><td>媒介实验室</td><td> H - 1200 </td><td></td></tr><tr><td>盖玻片</td><td> VWR </td><td> 631-1336 </td><td></td></tr></tbody></table>

single drosophila ommatidium dissection and imaging

果蝇神经科学的研究取得了宝贵的贡献，并已被广泛用作一种神经退行性疾病，因为其强大的遗传学1的模型。尤其是飞眼睛已选择的器官，神经退行性疾病的研究果蝇的神经系统是最方便和生活可有可无的部分。但是完整的眼睛主要需要注意的是难度，因为强烈的自体荧光的色素，在成像的细胞内事件，如自体吞噬动态 2，这是最重要的的神经退行性疾病的认识。 我们最近使用过的3单小眼的解剖和文化已经为我们的自噬功能障碍的认识中最重要的一个飞行模型Dentatorubro - Pallidoluysian萎缩 （ DRPLA）3，4。 现在，我们的报告全面描述了这种技术（图1），改编自电生理研究，这是有可能大幅扩大，神经退行性疾病的飞行模型的可能性。这种方法可适应图像活细胞内的事件，并监测到感光细胞的有效药物管理局（图2）。如果使用与镶嵌技法6-8相结合，可检测基因不同细胞的反应（图2）并行。

Watch this Scientific Journal Video about Single Drosophila Ommatidium Dissection and Imaging at JoVE.com

Single Drosophila Ommatidium Dissection and Imaging

在成人使用的限制因素<em&gt;果蝇</em&gt;眼来研究神经退行性疾病和细胞生物学，细胞内过程的困难成像。我们描述了单小眼的解剖，产生一个真正的初级神经细胞培养，可药物治疗和先进的成像。

单果蝇 Ommatidium解剖和影像

The fruit fly Drosophila melanogaster has made invaluable contributions to neuroscience research and has been used widely as a model for neurodegenerative ...

Research

JoVE Journal

Neuroscience

Single Drosophila Ommatidium Dissection and Imaging

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

在昆虫单感器记录果蝇按蚊</em

The sense of smell is essential for insects to find foods, mates, predators, and oviposition sites3. Insect olfactory sensory neurons (OSNs) are enclosed ...

科研

神经科学

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

Dissection and Imaging of Active Zones in the Drosophila Neuromuscular Junction

在活动区域​​的解剖和影像果蝇神经肌肉交界处

The Drosophila  larvae neuromuscular junction (NMJ) is an excellent model for the study of synaptic structure and function. Drosophila  is well known for ...

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

Dissection and Immunohistochemistry of Larval, Pupal and Adult Drosophila Retinas

幼虫，蛹和成人的解剖和免疫组化<em&gt;果蝇</em&gt;视网膜

The compound eye of Drosophila melanogaster consists of about 750 ommatidia (unit eyes). Each ommatidium is composed of about 20 cells, including ...

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

标记的单细胞在中枢神经系统<em&gt;果蝇</em

In this  article we describe how to individually label neurons in the embryonic CNS of Drosophila melanogaster by juxtacellular injection of the ...

A Single-fly Assay for Foraging Behavior in Drosophila

A Single-fly Assay for Foraging Behavior in Drosophila

单苍蝇检测方法的觅食行为的<em&gt;果蝇</em

For many animals, hunger promotes changes in the olfactory system in a manner that facilitates the search for appropriate food sources. In this video ...

Live Imaging of Drosophila Larval Neuroblasts

Live Imaging of Drosophila Larval Neuroblasts

的实时成像<em&gt;果蝇</em&gt;幼虫神经母细胞

Stem cells divide asymmetrically to generate two progeny cells with unequal fate potential: a self-renewing stem cell and a differentiating cell. Given ...

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

A Simple One-step Dissection Protocol for Whole-mount Preparation of Adult Drosophila Brains

一个简单的一步解剖协议成人全安装准备<em&gt;果蝇</em&gt;脑

There is an increasing interest in using Drosophila to model human brain degenerative diseases, map neuronal circuitries in adult brains, and study the ...

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

在体内果蝇前运动神经末端的单分子跟踪

An increasing number of super-resolution microscopy techniques are helping to uncover the mechanisms that govern the nanoscale cellular world. ...

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

Single-cell Resolution Fluorescence Live Imaging of Drosophila Circadian Clocks in Larval Brain Culture

单细胞分辨荧光活成像在幼虫脑培养中的果蝇昼夜节律时钟

The circadian pacemaker circuit orchestrates rhythmic behavioral and physiological outputs coordinated with environmental cues, such as day/night cycles. ...

Dissection of Single Skeletal Muscle Fibers for Immunofluorescent and Morphometric Analyses of Whole-Mount Neuromuscular Junctions

单骨骼肌肉纤维解剖，用于全山神经肌肉结的免疫荧光和形态分析

The neuromuscular junction (NMJ) is a specialized point of contact between the motor nerve and the skeletal muscle. This peripheral synapse exhibits high ...