Name: brain slice stimulation using a microfluidic network and standard perfusion chamber
Uploaded: 2007-10-01T00:00:00
Description: Watch this Scientific Journal Video about Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber at JoVE.com

<p>经费是由美国国立卫生研究院MH - 64611和NARSAD青年研究者奖。作者还想Beagley，马克Dikopf，亚当史密斯和Ben承认他们的技术援助。 </p>

<p class="jove_title"> SU - 8模具制造</p><p class="jove_step">硕士准备</p><ol><li>硅片衬底上的SU - 8的主人是准备使用两个步骤的标准消极抵制光刻工艺。 </li><li>被删除的硅片上的对准标记（位于外周边沿晶圆），使用这些结构的高度刀片超过实际设备结构。 </li><li>硅片，然后用异丙醇清洗和干燥后<sub>的</sub> N 2流。与胶带厚度小于最高的器件结构的支柱取代四边晶圆对准标记。 <br /> <strong><br />注：</strong>如果支柱的高度超?...

<ol>
	<li>Blake, A. J., Pearce, T. M., Rao, N. S., Johnson, S. M., Williams, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multilayer+PDMS+microfluidic+chamber+for+controlling+brain+slice+microenvironment.">Multilayer PDMS microfluidic chamber for controlling brain slice microenvironment.</a> <em style='font-style: italic'>Lab on a Chip</em>. <b>7</b>, 842-849 (2007).</li><li>Walker, G. M., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+passive+pumping+method+for+microfluidic+devices.">A passive pumping method for microfluidic devices.</a> <em style='font-style: italic'>Lab on a Chip</em>. <b>2</b>, 131-134 (2002).</li></ol>

<p>现有的宏观或微观的脑片灌注商会在他们提供公开与神经递质的大脑切片的空间分辨率是有限的。这里展示的微流体装置的技术克服了这个限制，使用简单的微机电技术。据预计，在微流体设备制造和易于集成与现有的电设置简单，使所显示的设备技术的广泛应用。有趣的实验是不可能的前面，可以与目前的微流体装置。脑片不同的微环境，可以接触到不同的神经递质，在不同的时间尺度。目前的原型设备包括?...

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		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>RC-26GPL</td>
<td>Tool</td>
<td>Warner Instruments</td>
<td>W2-64-0236</td>
<td>Low Profile Large Bath RC-26GLP Recording Chamber</td>
</tr>
<tr>
<td>SHD-26GH/10</td>
<td>Tool</td>
<td>Warner Instruments</td>
<td>W2-64-0253</td>
<td>Stainless steel slice hold-down for RC-26G, 1.0 mm thread spacing </td>
</tr>
<tr>
<td>PDMS (polydimethylsiloxane)</td>
<td>Reagent</td>
<td>Dow Corning</td>
<td>Sylgard 184</td>
<td>Silicone Elastomer Kit</td>
</tr>
<tr>
<td>Plasma Preen-II 862</td>
<td>Tool</td>
<td>Plasmatic Systems, Inc.</td>
<td>&nbsp;</td>
<td>Microwave plasma system</td>
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<td>Model P-1</td>
<td>Tool</td>
<td>Warner Instruments</td>
<td>W2-64-0277</td>
<td>Series 20 Plain Platform, Model P-1</td>
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<tr>
<td>SA-NIK</td>
<td>Tool</td>
<td>Warner Instruments</td>
<td>W2-64-0291</td>
<td>Adapter for Nikon Diaphot/TE200/TE2000, SA-NIK</td>
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<tr>
<td>Oxygenated, heated ACSF (Artificial cerebro-spinal fluid)</td>
<td>Reagent</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Exact composition will vary with application</td>
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brain slice stimulation using a microfluidic network and standard perfusion chamber

<p class="jove_content">我们已经证明了两个级别的微流体装置，可以很容易地与现有的电设置集成制造。两个级别的微流体装置是采用两个步骤的标准，消极<sup>抵制</sup>光刻工艺1。第一层次包含在每年年底与入口和出口微。第二个层次包含位于通道长度的中间，与通道宽度为中心沿微型圆孔。被动抽水的方法是用泵的液体，从进气口， <sup>出气</sup>口2。集成微流体装置与现成的灌注商会，允许无缝集成与电设置。在进气道流通过对出口口岸的微流体介绍，并通​​过位于顶部的微灌注洗澡的圆形开口也难逃。因此可以接触不同的神经递质的大脑切片置于灌注室浴及以上的微流体装置的底部表面。微流体装置的微型厚度和材料的透明性玻璃盖玻片和PDMS（聚二甲基硅氧烷）]用于制造微流体装置允许的大脑切片的显微镜。微流体装置，可以介绍到大脑切片的微环境的化学刺激（包括空间和时间）调制。 </p>

Watch this Scientific Journal Video about Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber at JoVE.com

Brain Slice Stimulation Using a Microfluidic Network and Standard Perfusion Chamber

<p class="jove_content">我们证明一个简单的微流体装置，可与标准的电设置综合揭露一个很好的控制方式不同的神经递质的大脑切片的微观表面加工。</p>

脑片的刺激，利用微流控网络和标准灌注腔

We have demonstrated the fabrication of a two-level microfluidic device that can be easily integrated with existing electrophysiology setups. The ...

Research

JoVE Journal

Biology

Fabrication of a Microfluidic Device for the Compartmentalization of Neuron Soma and Axons

条块索玛的神经元和轴突的一个微流体设备制造

In this video, we demonstrate the technique of soft lithography with polydimethyl siloxane (PDMS) which we use to fabricate a microfluidic device for ...

科研

生物学

Preparing E18 Cortical Rat Neurons for Compartmentalization in a Microfluidic Device

准备在微流体装置E18条块皮质大鼠神经元

In this video, we demonstrate the preparation of E18 cortical rat neurons. E18 cortical rat neurons are obtained from E18 fetal rat cortex previously ...

CD4+ T-Lymphocyte Capture Using a Disposable Microfluidic Chip for HIV

CD4 + T淋巴细胞艾滋病毒的捕获使用一次性微流体芯片

Cell Capture Using a Microfluidic Device

细胞捕获使用一个微流体装置

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

使用琼脂糖凝胶为基础的微流体装置的评估神经干细胞的运动

While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is ...

Trypsinizing and Subculturing Mammalian Cells

Trypsinizing和传代的哺乳动物细胞

As cells reach confluency, they must be subcultured or passaged. Failure to subculture confluent cells results in reduced mitotic index and eventually in ...

The Microfluidic Probe: Operation and Use for Localized Surface Processing

微探针：操作和使用的局部表面处理

Microfluidic devices allow assays to be performed using minute amounts of sample and have recently been used to control the microenvironment of cells. ...

Platelet Adhesion and Aggregation Under Flow using Microfluidic Flow Cells

根据流量的血小板粘附和聚集，使用微流控流细胞

Platelet aggregation occurs in response to vascular injury where the extracellular matrix below the endothelium has been exposed. The platelet adhesion ...

Mapping the After-effects of Theta Burst Stimulation on the Human Auditory Cortex with Functional Imaging

映射后的Theta突发刺激对人类听觉皮层功能成像

Auditory cortex pertains to the processing of sound, which is at the basis of speech or music-related processing1. However, despite considerable recent ...

Investigating Protein-protein Interactions in Live Cells Using Bioluminescence Resonance Energy Transfer

研究蛋白质 - 蛋白质相互作用活细胞使用生物发光共振能量转移

Assays based on Bioluminescence Resonance Energy Transfer (BRET) provide a sensitive and reliable means to monitor protein-protein interactions in live ...