Name: a mouse model of the cornea pocket assay for angiogenesis study
Uploaded: 2011-08-18T12:00:00
Description: Watch this Scientific Journal Video about A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study at JoVE.com

我们的研究是由美国国立卫生研究院，国家眼科研究所院内研究计划的支持。

所有的设备和试剂使用无菌。批准该协议是由动物护理和使用聂/ NIH委员会（ACUC）（动物研究协议NEI - 553），并根据美国国立卫生研究院的准则和法规执行。 1。产生血管生成因子含有颗粒在这一部分中，包含利益的血管生成因子的颗粒准备。 <ol><li>设为10％（W / V），硫糖铝，用PBS液。贮存在室温下。 </li><li>设为12％（W ​​/ V）聚甲基丙烯酸羟乙酯用...

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	<li>Cao, Y., Linden, P., Shima, D., Browne, F., Folkman, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+angiogenic+activity+and+hypoxia+induction+of+heterodimers+of+placenta+growth+factor/vascular+endothelial+growth+factor.">In vivo angiogenic activity and hypoxia induction of heterodimers of placenta growth factor/vascular endothelial growth factor.</a> The Journal of clinical investigation. 98, 2507-2511 (1996).</li><li>Rogers, M. S., Birsner, A. E., D'Amato, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mouse+cornea+micropocket+angiogenesis+assay.">The mouse cornea micropocket angiogenesis assay.</a> Nature. 2, 2545-2550 (2007).</li><li>Poche, R. A., Saik, J. E., West, J. L., Dickinson, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+mouse+cornea+as+a+transplantation+site+for+live+imaging+of+engineered+tissue+constructs.">The mouse cornea as a transplantation site for live imaging of engineered tissue constructs.</a> Cold Spring Harbor protocols. , 5416-54 (2010).</li><li>Zhang, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VEGF-B+is+dispensable+for+blood+vessel+growth+but+critical+for+their+survival,+and+VEGF-B+targeting+inhibits+pathological+angiogenesis.">VEGF-B is dispensable for blood vessel growth but critical for their survival, and VEGF-B targeting inhibits pathological angiogenesis.</a> Proceedings of the National Academy of Sciences of the United States of America. , 106-6152 (2009).</li><li>Tong, S., Yuan, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dose+response+of+angiogenesis+to+basic+fibroblast+growth+factor+in+rat+corneal+pocket+assay:+I.+Experimental+characterizations.">Dose response of angiogenesis to basic fibroblast growth factor in rat corneal pocket assay: I. Experimental characterizations.</a> Microvascular research. 75, 10-15 (2008).</li><li>Muthukkaruppan, V., Auerbach, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Angiogenesis+in+the+mouse+cornea.">Angiogenesis in the mouse cornea.</a> Science. 205, 1416-1418 (1979).</li><li>Ziche, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Corneal+assay+for+angiogenesis.">Corneal assay for angiogenesis.</a> Methods in molecular medicine. 46, 131-142 (2001).</li><li>Ziche, M., Morbidelli, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+corneal+pocket+assay.">The corneal pocket assay.</a> Methods in molecular biology. 467, 319-329 (2009).</li></ol>

鼠标角膜口袋检测模型是在1979年首次描述Muthukkaruppan VR和奥尔巴赫R 6。 2,3,7,8不同的实验室已经公布了详细的协议。我们的实验室已修改和 4多年来，在我们的研究使用这种方法。鼠标角膜口袋里的分析是一个很大的重复性相对简单的模型。这种模式的另一个优点是，由于角膜通常缺乏血管在这个实验中，有背景的最低金额。因此，这种模式通常用于研究不同的因素和分子的血管?...

<table border="1"><tbody><tr><th>设备</th><th>公司</th><th>目录编号</th></tr><tr><td>解剖显微镜</td><td>世界精密仪器</td><td> PZMIV - BS </td></tr><tr><td>冯格雷夫白内障刀（1.5x25mm刀片，平） </td><td>安布勒手术</td><td> 3401E </td></tr><tr><td>杜蒙＃5镊子</td><td>精细科学的工具</td><td> 11252-50 </td></tr><tr><td>裂隙灯</td><td>蔡司</td><td> SL - M 30 </td></tr></tbody></table> 表1设备<table border="1"><tbody><tr><th>试剂</th><th>公司</th><th>目录＃ </th><th>评论</th></tr><tr><td>碱性成纤维细胞生长因子（碱性成纤维细胞生长因子） </td><td> PEPROTECH INC。 </td><td> 100 - 18B </td><td></td></tr><tr><td>硫糖铝（蔗糖octasulfate铝复合） </td><td>西格玛</td><td> S0652 </td><td> 10％的PBS </td></tr><tr><td>聚甲基丙烯酸羟乙酯（聚（甲基丙烯酸2 - 羟乙基）） </td><td>西格玛</td><td> P3932 </td><td> 12％乙醇</td></tr></tbody></table> 表2。试剂和材料

a mouse model of the cornea pocket assay for angiogenesis study

一个正常的角膜是明确的血管组织。然而，血管可诱发，在强有力的血管生成因子1的角膜成长和生存。这种独特的角膜口袋检测最常用的血管生成研究模型之一。角膜细胞组成的多层次。因此，可以嵌入颗粒含有的血管生成因子在角膜上，探讨其血管生成作用 2,3利益。在这里，我们提供了一步一步示范如何（一）产生血管生成因子含颗粒（二）嵌入到角膜颗粒（三）分析利益的血管生成因子诱导的血管生成。由于碱性成纤维细胞生长因子（bFGF）是已知的最有力的血管生成因子之一，它是用来诱发角膜新生血管形成。

Watch this Scientific Journal Video about A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study at JoVE.com

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study

角膜是独特的，因为它没有血管组织。然而，强大的血管的生长和生存，可以在角膜上，通过强有力的血管生成因子诱导。因此，角膜可以与我们提供一个有价值的工具，对血管生成的研究。此协议演示了如何执行角膜口袋检测，以及如何评估使用这个模型的血管生成因子诱导的血管生成的小鼠模型。

一个小鼠模型角膜血管生成研究掌上含量

A normal cornea is clear of vascular tissues. However, blood vessels can be induced to grow and survive in the cornea when potent angiogenic factors are ...

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