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17.1K Views
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09:50 min
October 6th, 2011
DOI :
10.3791/3109-v
Chapters
0:05
Title
1:32
Brain Slice Preparation
2:17
Equipment Startup
3:24
Whole Cell Patch-clamp Recording
5:52
Laser Scanning Photostimulation
7:44
Representative Results for Electrophysiological Recording and Photostimulation Mapping
Summary
本稿では、限られた抑制性ニューロン集団でGFPを発現するトランスジェニックマウスにおける細胞全体の録音でレーザ走査光刺激を組み合わせたアプローチを紹介します。手法は特異的な阻害皮質ニューロンの局所シナプス回路の広範なマッピングと定量分析が可能になります。
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