Name: normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury
Uploaded: 2011-08-30T12:00:00
Description: Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

按照国家机构的研究和所有动物协议的俄勒冈卫生科学大学的体制的动物护理和使用委员会批准的护理和使用动物的健康指引所述的所有程序进行。 1。手术准备<ol><li>称量鼠标。描述的过程进行C57BL / 6小鼠体重20至25克使用3-4％的异氟醚诱导麻醉诱导，并随后保持1.5-2.5％异氟醚在空气/氧气混合。 </li><li>润滑眼睛的位置上加热垫的动物仰卧。用胶布固定4四肢。后腿的爪子可...

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常温下在小鼠心脏骤停和心肺复苏模型提供了一个模式，重复的病理生理及临床最常见的急性肾损伤，全身血流灌注不足引起的形态的多种渠道的评价。假设检验，可访问的遗传操作技术和充分理解和特点的解剖和生理学实验小鼠的一整套资助。 这里描述的，对于有经验的生存率是80％。虽然手术的准备和逮捕后的护理很简单，被捕鼠标的实际复苏是一个具有挑战性的手术技...

<table border="1"><tbody><tr><th>试剂名称</th><th>公司</th><th>目录编号</th></tr><tr><td>氯化钾</td><td>雅培医疗</td><td> 06653-5 </td></tr><tr><td>异氟醚</td><td>雅培医疗</td><td> 05260-05（和其他） </td></tr><tr><td>肾上腺素盐酸</td><td>多</td><td>多</td></tr><tr><td>从DIGI -感温度控制器</td><td>科尔 - 帕默</td><td> EW - 89000 - 00 </td></tr><tr><td> Angiocath四蛋白酶</td><td> VWR </td><td> 381134 </td></tr><tr><td> Frova角度呼吸道介绍人</td><td>厨师</td><td> G27282 </td></tr><tr><td>对小鼠的MicroVent换气扇</td><td>哈佛器械</td><td> 733591 </td></tr></tbody></table>

normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury

5,6全身低灌注成功繁殖，在啮齿类动物模型急性肾损伤（AKI）是一个共同的，极具杀伤力，危重病的并发症， 死亡率高 1-4，这是最经常全身血流灌注不足引起的。一直困难重重。7-9,9,10聘请局灶性脑缺血模型反复论证的结果没有转化为临床上，和较大的动物模型，全身血流灌注不足无法获得的遗传操纵的工具在鼠标的可能。11,12然而，心脏骤停和心肺复苏的小鼠模型，近年来又出现了可适应模型 AKI的13这种模式可靠地再现生理功能，解剖和组织学在临床AKI的看到的结果，迅速重复，并提供所有手术小鼠模型的显着优点，包括访问的基因操纵技术，成本相对低的大型动物，和易用性。使用这种模式，集团已开发的丰富经验，以评估在AKI的一些器官的具体成果。 14,15

Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury (Video) | JoVE

一个强大的围手术期和关键护理相关的急性肾损伤模型。使用近复制临床AKI的组织学和功能的变化有可能诱发心脏骤停的全身血流灌注不足。

常温心脏骤停和心肺复苏术：缺血再灌注损伤的小鼠模型

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

Research

JoVE Journal

Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice (Video) | JoVE

急性肾损伤和伤后纤维化小鼠缺血再灌注模型

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

科研

医学

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

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诱导和缺血再灌注损伤评估中的Langendorff灌注鼠心

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

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肠缺血再灌注损伤的小鼠模型

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

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视网膜缺血再灌注损伤的小鼠模型通过眼压升高

Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses (Video) | JoVE

在鼠耳皮肤的免疫反应的活体多光子成像诱导缺血再灌注损伤

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Ultrasonographic Assessment During Cardiopulmonary Resuscitation

Ultrasonographic Assessment During Cardiopulmonary Resuscitation (Video) | JoVE

心肺复苏期间的超分谱评估

The US-CAB (Ultrasound, Circulation/Airway/Breathing) protocol integrates several sonographic techniques into a structured assessment of the circulation, ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery (Video) | JoVE

重建显微外科缺血再灌注损伤研究的临床前大鼠模型

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model (Video) | JoVE

在穆林模型中缺血再灌注损伤后干细胞的心肌内传递延迟

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

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器官缺血-输液损伤模拟大鼠肝脏移植模型的血动力学变化

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion (Video) | JoVE

猪缺血/再灌注模型中脊髓微灌注的实时评估

Spinal cord injury is a devastating complication of aortic repair. Despite developments for the prevention and treatment of spinal cord injury, its ...