Name: normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury
Uploaded: 2011-08-30T12:00:00
Description: Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Alle beschriebenen Verfahren sind in Übereinstimmung mit den National Institutes of Health Richtlinien für die Pflege und Nutzung von Tieren in der Forschung und allen tierischen Protokolle wurden von der Oregon Health &amp; Science University Institutional Animal Care und Verwenden Ausschusses zugelassen wurden. 1. OP-Vorbereitung <ol><li> Wiegen Sie die Maus. Das beschriebene Verfahren basiert auf C57BL / 6 Mäuse mit einem Gewicht zwischen 20 und 25 g. durchgeführt Anästhesie ist in...

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Die normothermen Modell Herzstillstand und Reanimation in der Maus bietet eine Vielzahl von Möglichkeiten der Auswertung in einem Modell, welches die Pathophysiologie und Morphologie der häufigste klinische Ursache AKI, Ganzkörper-Hypoperfusion repliziert. Das Testen von Hypothesen kann durch den Zugang zu einer breiten Palette von genetischen Manipulationstechniken und gut verstanden und charakterisiert Anatomie und Physiologie der Labormaus unterstützt werden. Wie hier beschrieben, ist...

<table border="1"><tbody><tr><th> Name des Reagenzes </th><th> Firma </th><th> Katalog-Nummer </th></tr><tr><td> Kaliumchlorid </td><td> Abbott Medical </td><td> 06653-5 </td></tr><tr><td> Isofluran </td><td> Abbott Medical </td><td> 05260-05 (und andere) </td></tr><tr><td> Adrenalin HCl </td><td> Mehrere </td><td> Mehrere </td></tr><tr><td> Digi-Sense-Temperaturregler </td><td> Cole-Palmer </td><td> EW-89000-00 </td></tr><tr><td> Angiocath IV Cath </td><td> VWR </td><td> 381134 </td></tr><tr><td> Frova abgewinkelt Atemwege Einführschleuse </td><td> Kochen </td><td> G27282 </td></tr><tr><td> MicroVent Ventilator für Mäuse </td><td> Harvard Apparatus </td><td> 733591 </td></tr></tbody></table>

normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury

Akute Nierenschädigung (AKI) ist ein verbreitetes, hoch tödliche Komplikation der kritischen Erkrankung, die eine hohe Mortalität 1-4 und die am häufigsten von Ganzkörper-Hypoperfusion verursacht hat. 5,6 Erfolgreiche Reproduktion des ganzen Körpers Hypoperfusion in Nagermodellen wurde mit Schwierigkeiten verbunden. 7-9,9,10 Models, die fokale Ischämie beschäftigen immer wieder Ergebnisse, die nicht dem klinischen Umfeld, und größere Tiermodelle, die für den ganzen Körper Hypoperfusion keinen Zugang zu den vollständigen Toolset der genetischen Manipulation erlauben übersetzen gezeigt möglich in der Maus. 11,12 Doch in den letzten Jahren einem Mausmodell der Herzstillstand und Reanimation entstanden die zu Modell AKI angepasst werden. 13 Dieses Modell zuverlässig reproduziert physiologische, funktionale, anatomische und histologische Ergebnisse in klinischen AKI gesehen , wird schnell wiederholbar, und bietet alle wichtigen Vorteile einer murinen chirurgischen Modells, einschließlich des Zugangs zu genetischen manipulativen Techniken, niedrige Kosten im Vergleich zu großen Tieren, und Benutzerfreundlichkeit. Unsere Gruppe verfügt über umfangreiche Erfahrungen mit der Anwendung dieses Modells entwickelt, um eine Reihe von Organ-spezifische Ergebnisse in AKI beurteilen. 14,15

Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

Ein leistungsfähiges Modell für perioperative und Intensivmedizin akute Nierenversagen wird vorgestellt. Mit ganzen Körper Hypoperfusion durch Herzstillstand ist es möglich, fast replizieren die histologische und funktionelle Veränderungen der klinischen AKI induziert.

Normothermen Herzstillstand und Herz-Lungen: Ein Maus-Modell der Ischämie-Reperfusionsschaden

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

Research

JoVE Journal

Medicine

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Ischämie-Reperfusion-Modell des akuten Nierenschädigung und nach der Verletzung Fibrose bei Mäusen

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

Forschung

Medizin

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Induktion und Beurteilung der Ischämie-Reperfusion Injury in Langendorff-perfundierten Rattenherzen

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

Murine Modell der intestinalen Ischämie-Reperfusionsschaden

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Ein Maus-Modell der Netzhautischämie-Reperfusionsschaden Durch Erhöhung des intraokularen Druck

Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Induzierende Ischemia-Reperfusionsschaden in der Maus-Ohr-Haut für intravital Multiphotonenionisation Imaging von Immunantworten

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Ultrasonographic Assessment During Cardiopulmonary Resuscitation

Ultrasonografische Beurteilung bei kardiopulmonaler Reanimation

The US-CAB (Ultrasound, Circulation/Airway/Breathing) protocol integrates several sonographic techniques into a structured assessment of the circulation, ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Ein präklinisches Rattenmodell zur Untersuchung von Ischämie-Reperfusionsverletzungen in der rekonstruktiven Mikrochirurgie

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Verzögerte intramyokardiale Lieferung von Stammzellen nach Ischämie-Reperfusionsverletzung in einem Murine-Modell

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

OrganIschemia-Reperfusion Verletzung durch Simulation hämodynamischer Veränderungen im Lebertransplantationsmodell der Ratte

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion

Echtzeit-Beurteilung der Rückenmarksmikroperfusion in einem Schweinemodell der Ischämie/Reperfusion

Spinal cord injury is a devastating complication of aortic repair. Despite developments for the prevention and treatment of spinal cord injury, its ...