Name: normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury
Uploaded: 2011-08-30T12:00:00
Description: Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Tutte le procedure descritte sono condotte in conformità con gli Istituti Nazionali di Sanità le linee guida per la cura e l&#39;uso di animali nella ricerca e tutti i protocolli di animali sono stati approvati dalla Oregon Health &amp; Science Animal Care dell&#39;Università e del Comitato Istituzionale Usa. 1. Preparazione chirurgica <ol><li> Pesare il mouse. La procedura descritta viene eseguita su topi C57BL / 6 di peso compreso tra 20 e 25 g. L&#39;anestesia viene indotta in una sc...

<ol>
	<li>Chertow, G. M., Lazarus, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Predictors+of+mortality+and+the+provision+of+dialysis+in+patients+with+acute+tubular+necrosis.+the+auriculin+anaritide+acute+renal+failure+study+group.">Predictors of mortality and the provision of dialysis in patients with acute tubular necrosis. the auriculin anaritide acute renal failure study group.</a> J Am Soc Nephrol. 9, 692-698 (1998).</li><li>Lassnigg, A., Schmidlin, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Minimal+changes+of+serum+creatinine+predict+prognosis+in+patients+after+cardiothoracic+surgery:+A+prospective+cohort+study.">Minimal changes of serum creatinine predict prognosis in patients after cardiothoracic surgery: A prospective cohort study.</a> J Am Soc Nephrol. 15, 1597-1605 (2004).</li><li>Lassnigg, A., Donner, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lack+of+renoprotective+effects+of+dopamine+and+furosemide+during+cardiac+surgery.">Lack of renoprotective effects of dopamine and furosemide during cardiac surgery.</a> J Am Soc Nephrol. 11, 97-104 (2000).</li><li>Metnitz, P. G., Krenn, C. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+acute+renal+failure+requiring+renal+replacement+therapy+on+outcome+in+critically+ill+patients.">Effect of acute renal failure requiring renal replacement therapy on outcome in critically ill patients.</a> Crit Care Med. 30, 2051-2058 (2002).</li><li>Uchino, S., Kellum, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+renal+failure+in+critically+ill+patients:+A+multinational,+multicenter+study.">Acute renal failure in critically ill patients: A multinational, multicenter study.</a> JAMA. 294, 813-818 (2005).</li><li>Mehta, R. L., Pascual, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Spectrum+of+acute+renal+failure+in+the+intensive+care+unit:+The+PICARD+experience.">Spectrum of acute renal failure in the intensive care unit: The PICARD experience.</a> Kidney Int. 66, 1613-1621 (2004).</li><li>Zager, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Partial,+aortic+ligation:+A+hypoperfusion+model+of+ischemic+acute+renal+failure+and+a+comparison+with+renal+artery+occlusion.">Partial, aortic ligation: A hypoperfusion model of ischemic acute renal failure and a comparison with renal artery occlusion.</a> J Lab Clin Med. 110, 396-405 (1987).</li><li>Zager, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adenine,+nucleotide+changes+in+kidney,+liver,+and+small+intestine+during+different+forms+of+ischemic+injury.">Adenine, nucleotide changes in kidney, liver, and small intestine during different forms of ischemic injury.</a> Circ Res. 68, 185-196 (1991).</li><li>Oliver, J., MacDowell, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pathogenesis+of+acute+renal+failure+associated+with+traumatic+and+toxic+injury;+renal+ischemia,+nephrotoxic+damage+and+the+ischemic+episode.">The pathogenesis of acute renal failure associated with traumatic and toxic injury; renal ischemia, nephrotoxic damage and the ischemic episode.</a> J Clin Invest. 30, 1307-1439 (1951).</li><li>Phillips, R. A., Dole, V. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+acute+hemorrhage+and+traumatic+shock+on+renal+function+in+dogs.">Effects of acute hemorrhage and traumatic shock on renal function in dogs.</a> Am J Physiol. 145, 314-336 (1945).</li><li>Klocke, R., Tian, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+animal+models+of+heart+failure+related+to+coronary+heart+disease.">Surgical animal models of heart failure related to coronary heart disease.</a> Cardiovasc Res. 74, 29-38 (2007).</li><li>Traystman, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+focal+and+global+cerebral+ischemia.">Animal models of focal and global cerebral ischemia.</a> ILAR J. 44, 85-95 (2003).</li><li>Burne-Taney, M. J., Kofler, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+renal+failure+after+whole+body+ischemia+is+characterized+by+inflammation+and+T+cell-mediated+injury.">Acute renal failure after whole body ischemia is characterized by inflammation and T cell-mediated injury.</a> Am J Physiol Renal Physiol. 285, 87-94 (2003).</li><li>Hutchens, M. P., Nakano, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen+is+renoprotective+via+a+non-receptor+dependent+mechanism+after+cardiac+arrest+in+vivo.">Estrogen is renoprotective via a non-receptor dependent mechanism after cardiac arrest in vivo.</a> Anesthesiology. 112, 395-405 (2010).</li><li>Hutchens, M. P., Nakano, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Soluble+epoxide+hydrolase+gene+deletion+reduces+survival+after+cardiac+arrest+and+cardiopulmonary+resuscitation.">Soluble epoxide hydrolase gene deletion reduces survival after cardiac arrest and cardiopulmonary resuscitation.</a> Resuscitation. 76, 89-94 (2007).</li><li>Planta, I. v. o. n., Weil, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cardiopulmonary+resuscitation+in+the+rat.">Cardiopulmonary resuscitation in the rat.</a> J Appl Physiol. 65, 2641-2647 (1988).</li><li>Cobb, L. A., Fahrenbruch, C. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+cardiopulmonary+resuscitation+prior+to+defibrillation+in+patients+with+out-of-hospital+ventricular+fibrillation.">Influence of cardiopulmonary resuscitation prior to defibrillation in patients with out-of-hospital ventricular fibrillation.</a> JAMA. 281, 1182-1188 (1999).</li><li>Aufderheide, T. P., Lurie, K. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Death+by+hyperventilation:+A+common+and+life-threatening+problem+during+cardiopulmonary+resuscitation.">Death by hyperventilation: A common and life-threatening problem during cardiopulmonary resuscitation.</a> Crit Care Med. 32, 345-351 (2004).</li><li>Aufderheide, T. P., Sigurdsson, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hyperventilation-induced+hypotension+during+cardiopulmonary+resuscitation.">Hyperventilation-induced hypotension during cardiopulmonary resuscitation.</a> Circulation. 109, 1960-1965 (2004).</li></ol>

Il modello normotermico di arresto cardiaco e rianimazione cardiopolmonare nel topo offre molteplici vie di valutazione in un modello che riproduce la fisiopatologia e la morfologia della causa clinica più comune di AKI, tutto il corpo ipoperfusione. La verifica di ipotesi può essere aiutata da accesso a tutta una serie di tecniche di manipolazione genetica e l&#39;anatomia ben capito e caratterizzata e fisiologia del topo di laboratorio. Come descritto qui, la sopravvivenza in mani espert...

<table border="1"><tbody><tr><th> Nome del reagente </th><th> Azienda </th><th> Numero di catalogo </th></tr><tr><td> Cloruro di potassio </td><td> Abbott Medical </td><td> 06653-5 </td></tr><tr><td> Isoflurano </td><td> Abbott Medical </td><td> 05260-05 (e altri) </td></tr><tr><td> Epinefrina HCl </td><td> Multiplo </td><td> Multiplo </td></tr><tr><td> Digi-Sense Regolatore di temperatura </td><td> Cole-Palmer </td><td> EW-89000-00 </td></tr><tr><td> Angiocath IV Cath </td><td> VWR </td><td> 381134 </td></tr><tr><td> Frova introduttore vie ad angolo </td><td> Cucinare </td><td> G27282 </td></tr><tr><td> Ventilatore MicroVent per il Mice </td><td> Harvard Apparatus </td><td> 733591 </td></tr></tbody></table>

normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury

Danno renale acuto (AKI) è un comune, altamente letale, complicazione di malattia critica che ha un alto tasso di mortalità 1-4 e che è più frequentemente causata da tutto il corpo ipoperfusione. 5,6 riproduzione di successo di tutto il corpo ipoperfusione in modelli di roditori è stato irto di difficoltà. 7-9,9,10 modelle che impiegano ischemia focale hanno ripetutamente dimostrato che i risultati non si traducono in ambito clinico, e in modelli animali più grandi che permettono di accedere a tutta la mancanza ipoperfusione corpo al set di strumenti completo di manipolazione genetica possibile nel topo. 11,12 Tuttavia, negli ultimi anni un modello murino di arresto cardiaco e rianimazione cardiopolmonare è emerso che possono essere adattati a modello AKI. 13 Questo modello riproduce affidabile fisiologico, risultati funzionali, anatomiche e istologiche visto in clinica AKI , si sta rapidamente ripetibile, e offre tutti i vantaggi di un modello murino chirurgico, compreso l&#39;accesso alle tecniche di manipolazione genetica, relativamente a basso costo per grandi animali, e facilità d&#39;uso. Il nostro gruppo ha sviluppato una vasta esperienza con l&#39;utilizzo di questo modello per valutare una serie di organo-specifici risultati di AKI. 14,15

Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

Un modello potente per perioperatoria e critica lesioni legate ai reni per acuti è presentato. Utilizzando tutto il corpo ipoperfusione indotta da arresto cardiaco è possibile replicare quasi alterazioni istologiche e funzionali del clinico AKI.

Normotermico arresto cardiaco e rianimazione cardiopolmonare: un modello murino di ischemia-riperfusione

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

Research

JoVE Journal

Medicine

Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Ischemia-riperfusione Modello di danno renale acuta e post Infortunio fibrosi nei topi

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

Ricerca

Medicina

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Induzione e valutazione di ischemia-riperfusione Injury in Langendorff-perfusi Hearts Rat

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

Modello murino di ischemia-riperfusione intestinale Injury

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Un modello murino di retina ischemia-riperfusione lesioni a causa di aumento della pressione intraoculare

Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Inducendo ischemia-riperfusione lesioni in Ear pelle mouse per intravitale Multiphoton Imaging delle risposte immunitarie

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Ultrasonographic Assessment During Cardiopulmonary Resuscitation

Valutazione ultrasonografica durante la rianimazione cardiopolmonare

The US-CAB (Ultrasound, Circulation/Airway/Breathing) protocol integrates several sonographic techniques into a structured assessment of the circulation, ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Un modello di ratto preclinico per lo studio delle lesioni ischemia-reperfusione in microchirurgia ricostruttiva

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Consegna intramiocardica ritardata di cellule staminali dopo l'infortunio di ricottamento ischemia in un modello Murine

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Lesione da ischemia-riperfusione dell'organo simulando cambiamenti emodinamici nel modello di trapianto di fegato di ratto

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion

Valutazione in tempo reale della microperfusione del midollo spinale in un modello suino di ischemia/riperfusione

Spinal cord injury is a devastating complication of aortic repair. Despite developments for the prevention and treatment of spinal cord injury, its ...