Name: normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury
Uploaded: 2011-08-30T12:00:00
Description: Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Açıklanan tüm prosedürler uyarınca, araştırma ve tüm hayvan protokolleri Oregon Sağlık ve Bilim Üniversitesi Kurumsal Hayvan Bakım ve Kullanım Kurulu tarafından kabul edildi hayvanların bakımı ve kullanımı için Sağlık kılavuzların National Institutes yapılmaktadır. 1. Cerrahi Hazırlık <ol><li> Fare tartılır. Açıklanan prosedür g. 20 ile 25 arasında tartım C57BL / 6 fareler üzerinde yapılır Anestezi indüksiyon kutusunda izofluran% 3-4 kullanarak bir ne...

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Fare kardiyak arrest ve kardiyopulmoner resüsitasyon normotermik model AKI, tüm vücut hipoperfüzyon, en sık görülen klinik nedeni fizyopatolojisi ve morfolojisi çoğaltır bir model değerlendirme birden fazla yollar sunar. Hipotez testleri, genetik manipülasyon teknikleri ve iyi anlaşılmalı ve karakterize bir anatomisi ve fizyolojisi laboratuvar faresi bir yelpazesine erişim destekli olabilir. Burada açıklandığı gibi, deneyimli ellerde sağkalım% 80&#39;dir. Cerrahi hazı...

<table border="1"><tbody><tr><th> Reaktifin Adı </th><th> Şirket </th><th> Katalog numarası </th></tr><tr><td> Potasyum Klorür </td><td> Abbott Medikal </td><td> 06653-5 </td></tr><tr><td> İsofluranın </td><td> Abbott Medikal </td><td> 05.260-05 (ve diğerleri) </td></tr><tr><td> Epinefrin HCl </td><td> Çoklu </td><td> Çoklu </td></tr><tr><td> Digi-Sense sıcaklık kontrolörü </td><td> Cole-Palmer </td><td> EW-89000-00 </td></tr><tr><td> Angiocath IV Cath </td><td> VWR </td><td> 381134 </td></tr><tr><td> Frova açılı havayolu introduser </td><td> Aşçı </td><td> G27282 </td></tr><tr><td> Fareler için MicroVent Vantilatör </td><td> Harvard Apparatus </td><td> 733591 </td></tr></tbody></table>

normothermic cardiac arrest and cardiopulmonary resuscitation: a mouse model of ischemia-reperfusion injury

Akut Böbrek Sakatlık (AKI) yüksek mortalite 1-4 ve tüm vücut hipoperfüzyon tarafından en sık neden olduğu ortak bir kritik hastalık, oldukça ölümcül bir komplikasyon. Kemirgen modelleri tüm vücut hipoperfüzyon 5,6 Başarılı üreme zorluklarla dolu olmuştur. fokal iskemi istihdam 7-9,9,10 modeller art arda klinik ortamda ve genetik manipülasyon tam araç seti tüm vücut hipoperfüzyon eksikliği erişim için izin büyük hayvan modellerinde çevirmek olmayan sonuçları göstermiştir fare mümkün. 11,12 Ancak, son yıllarda, kardiyak arrest ve kardiyopulmoner resüsitasyon bir fare modeli modeli AKI adapte edilebileceği ortaya çıkmıştır. 13 Bu model güvenilir klinik AKI görülen fizyolojik, fonksiyonel, anatomik ve histolojik sonuçlar üretir hızla tekrarlanabilir ve genetik manipülatif teknikleri, büyük hayvanlar için düşük maliyetli akraba ve kullanım kolaylığı erişimi de dahil olmak üzere tüm cerrahi bir fare modelinin önemli avantajlar sunuyor. Grubumuz bu modelin kullanımı ile AKI, organ-spesifik sonuçlar bir sayısını değerlendirmek için geniş bir deneyime sahiptir. 14,15

Watch this Scientific Journal Video about Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury at JoVE.com

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

Perioperatif ve yoğun bakım ile ilgili akut böbrek hasarı için güçlü bir model sundu. Neredeyse, klinik AKI, histolojik ve fonksiyonel değişiklikleri çoğaltmak mümkündür kardiyak arrest ile indüklenen tüm vücut hipoperfüzyon kullanma.

Normotermik Kardiyak Arrest ve Kardiyopulmoner Resusitasyon: İskemi-Reperfüzyon Hasarı Fare Modeli

Acute Kidney Injury (AKI) is a common, highly lethal, complication of critical illness which has a high mortality1-4 and which is most 
frequently caused ...

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Ischemia-reperfusion Model of Acute Kidney Injury and Post Injury Fibrosis in Mice

Farelerde Akut Böbrek Hasarı ve Post Yaralanma Fibrozis iskemi-reperfüzyon Model

Ischemia-reperfusion induced acute kidney injury (IR-AKI) is widely used as a model of AKI in mice, but results are often quite variable with high, often ...

Induction and Assessment of Ischemia-reperfusion Injury in Langendorff-perfused Rat Hearts

Langendorff-perfüze sıçan Hearts indüksiyon ve İskemi-reperfüzyon Hasarı Değerlendirilmesi

The biochemical events surrounding ischemia reperfusion injury in the acute setting are of great importance to furthering novel treatment options for ...

Murine Model of Intestinal Ischemia-reperfusion Injury

Bağırsak İskemi-reperfüzyon Hasarı Murin Modeli

Intestinal ischemia is a life-threatening condition associated with a broad range of clinical conditions including atherosclerosis, thrombosis, ...

A Mouse Model of Retinal Ischemia-Reperfusion Injury Through Elevation of Intraocular Pressure

Göz İçi Basıncı Yükselmesi ile Retina İskemi-Reperfüzyon Hasarı Fare Modeli

Retinal ischemia-reperfusion (I/R) is a pathophysiological process contributing to cellular damage in multiple ocular conditions, including glaucoma, ...

Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses

Bağışıklık Yanıtları intravital multiphoton Görüntüleme Fare Kulak Skin uyaran İskemi-reperfüzyon yaralanması

Ischemia-reperfusion injury (IRI) occurs when there is transient hypoxia due to the obstruction of blood flow (ischemia) followed by a subsequent ...

Ultrasonographic Assessment During Cardiopulmonary Resuscitation

Kardiyopulmoner Resüsitasyon Sırasında Ultrasonografik Değerlendirme

The US-CAB (Ultrasound, Circulation/Airway/Breathing) protocol integrates several sonographic techniques into a structured assessment of the circulation, ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Rekonstrüktif Mikrocerrahide İskemi-Reperfüzyon Hasarı Nın İnceleştirilmesinde Klinik Öncesi Sıçan Modeli

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Delayed Intramyocardial Delivery of Stem Cells after Ischemia Reperfusion Injury in a Murine Model

Bir Murine Modelinde İskemi Reperfüzyon Yaralanması Sonrası Kök Hücrelerin Gecikmiş İntramiyokardiyal Teslimi

There is significant interest in the use of stem cells (SCs) for the recovery of cardiac function in individuals with myocardial injuries. Most commonly, ...

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model

Sıçan Karaciğer Nakli Modelinde Hemodinamik Değişiklikleri Simüle Ederek Organ İskemi-Reperfüzyon Yaralanması

Orthotopic liver transplantation (OLT) in rats is a tried and proven animal model used for preoperative, intraoperative, and postoperative studies, ...

Real-Time Assessment of Spinal Cord Microperfusion in a Porcine Model of Ischemia/Reperfusion

İskemi/Reperfüzyon Porsin Modelinde Omurilik Mikroperfüzyonunun Gerçek Zamanlı Değerlendirilmesi

Spinal cord injury is a devastating complication of aortic repair. Despite developments for the prevention and treatment of spinal cord injury, its ...