Ikke-Laser Capture Mikroskopi Tilgang til mikrodissektion af diskrete Mouse Brain Regioner for Total RNA Isolation og downstream næste generation sekventering og Gene Expression Profiling

RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.