Non-Laser Capture Mikroskopi Approach for mikrodisseksjon av Discrete Mouse Brain Regions for Total RNA Isolation og nedstrøms Next-Generation Sekvensering og Gene Expression Profiling

RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.