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Sin láser y de captura de enfoque de Microscopía de la microdisección de regiones discretas del cerebro del ratón para el aislamiento de ARN total y Downstream secuenciación de próxima generación y de perfiles de expresión génica

DOI :

10.3791/3125-v

November 13th, 2011

November 13th, 2011

14,439 Views

1Center for Sleep and Circadian Biology, Northwestern University

RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method.

Tags

Non laser Capture Microscopy

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