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21.4K Views
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12:18 min
July 22nd, 2011
DOI :
10.3791/3130-v
Chapters
0:05
Title
2:00
Preparation of Whole Cell Template
3:29
PCR Set-up
6:45
Gel Electrophoresis
9:20
Multiplexing PCR Results: Identifying Specific O and H Alleles Associated with S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
11:51
Conclusion
Summary
我们描述了一个多重PCR快速检测沙门氏菌血清型肠炎,哈达尔,海德堡和鼠伤寒沙门氏菌。具体的沙门氏菌血清型,可确定的目标是多重PCR技术,以独特的O -抗原生物合成集群和一个给定的血清型的鞭毛的基因和序列。血清型,然后分配到一个隔离基础上的具体化,规模扩增产物(PCR产物)对应的目标等位基因外观的沙门氏菌。
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