Name: title cell encapsulation by droplets
Uploaded: 2007-10-01T13:09:54
Description: Watch this Scientific Journal Video about Title Cell Encapsulation by Droplets at JoVE.com

<p class="jove_title"> NIH3T3 preparação células: </p><p class="jove_step"> Células de A. de ejeção </p><ol><li> Trypsinize células, então diluir 1:1 com media de células, e transferência de um frasco T75 para um tubo Falcon 15 mL </li><li> Spin down células em um pellet por centrifugação, aspirar o sobrenadante e lavar as células com DPBS </li><li> Spin down células em uma pelota de novo, e aspirar o sobrenadante </li><li> Ressuspender as células em meios de comunicação </li><li> Determinar a densidade de células com hemocit...

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		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Fluorescent Microscope</td>
<td>&nbsp;</td>
<td>Nikon</td>
<td>Eclipse TE-2000 U</td>
<td>&nbsp;</td>
<tr>
<td>Solenoid valve cell ejector</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Operation frequency: 1 KHz, 30psi, 50nl~0.5ul. 12V Valve Driver: 2.5 Amp drive current
</td>
</tr>
<tr>
<td>5-Gallon Portable air tank</td>
<td>&nbsp;</td>
<td>Coleman</td>
<td>Powermate CT5</td>
<td>Pressurized air: 30 PSI </td>
</tr>
<tr>
<td>Pressure regulators</td>
<td>&nbsp;</td>
<td>Marsh Bellofram</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Pulse Generator</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>HP8112A</td>
<td>Actuation frequency generation: 50 MHz
</td>
</tr>
<tr>
<td>XYZ-stage</td>
<td>&nbsp;</td>
<td>Newmark Systems</td>
<td>&nbsp;</td>
<td>With Stepping motor: 6inch travel xy-stage(NLS4-6-25), 2.5inch travel z-stage(NLS4-2.5-25), 3axis controller(NSC-G3), 0.1um resolution</td>
</tr>
<tr>
<td>NIH 3T3</td>
<td>Cell-line</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>fibroblasts</td>
</tr>
<tr>
<td>Trypsin</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>0.05% solution</td>
</tr>
<tr>
<td>NIH 3T3 cell medium</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>DPBS</td>
<td>Buffer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>T75</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>Tissue culture flasks</td>
</tr>
<tr>
<td>Plastic conical tubes</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>15 ml, for tissue culture</td>
</tr>		</tbody>
		</table>
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title cell encapsulation by droplets

Watch this Scientific Journal Video about Title Cell Encapsulation by Droplets at JoVE.com

Title Cell Encapsulation by Droplets

Encapsulation título celular por Droplets

Research

JoVE Journal

Biology

Derivation of Human Embryonic Stem Cells by Immunosurgery

Derivação de células estaminais embrionárias humanas por Immunosurgery

The ability of human embryonic stem cells to self-renew and differentiate into all cell types of 
the body suggests that they hold great promise for both ...

Biologia

Measuring Plant Cell Wall Extension (Creep) Induced by Acidic pH and by Alpha-Expansin

Measuring Plant Cell Wall Extension Creep Induced by Acidic pH and by Alpha-Expansin

Medição Usina Extensão parede celular (Creep) induzida pelo pH ácido e por Alpha-Expansin

Growing plant cell walls characteristically exhibit a property known as 'acid growth', by which we mean they are more extensible at low pH (&lt; 5) 1. The ...

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering 

O encapsulamento celular em Hidrogéis 3D para Engenharia de Tecidos

The 3D encapsulation of cells within hydrogels represents an increasingly important and popular technique for culturing cells and towards the development ...

PuraMatrix Encapsulation of Cancer Cells

Encapsulation PuraMatrix de células de câncer

Increasing evidence suggests that culturing cancer cells in three dimensions more accurately recapitulates the complexity of tumor biology. Many of these ...

Fate Mapping of Human Embryonic Stem Cells by Teratoma Formation

Mapeamento de destino de células estaminais embrionárias humanas por formação de teratomas

Human embryonic stem cells (hESCs) have an unlimited capacity for self-renewal, and the ability to differentiate into cells derived from all three ...

Detection of Post-translational Modifications on Native Intact Nucleosomes by ELISA

Detecção de modificações pós-translacionais sobre Native Nucleossomos Intact por ELISA

The genome of eukaryotes exists as chromatin which contains both DNA and proteins. The fundamental unit of chromatin is the nucleosome, which contains 146 ...

<em>In Vivo</em> 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

<em>In Vivo</em> Rastreamento 4-Dimensional de tronco hematopoéticas e células progenitoras em adultos mouse na calota craniana de Medula Óssea

Through a delicate balance between quiescence and proliferation, self renewal and production of differentiated progeny, hematopoietic stem cells (HSCs) ...

Generation of Transgenic <em>Hydra</em> by Embryo Microinjection

Generation of Transgenic Hydra by Embryo Microinjection

Geração de Transgênicos<em&gt; Hydra</em&gt; Por microinjeção de embriões

As a member of the phylum Cnidaria, the sister group to all bilaterians, Hydra can shed light on fundamental biological processes shared among ...

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

Fecal Análise de glicocorticóides: Non-invasive Monitoring Adrenal em eqüídeos

Adrenal activity can be assessed in the equine species by analysis of feces for corticosterone metabolites. During a potentially aversive situation, ...

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Reconstituição do Basic mitótico Fusos no esféricas Emulsão Gotas

Mitotic spindle assembly, positioning and orientation depend on the combined forces generated by microtubule dynamics, microtubule motor proteins and ...