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DOI :
10.3791/3190-v
September 30th, 2011
Chapters
0:05
Title
1:07
Cell Harvest and Lysis
1:55
Ni-affinity Purification of Hsp104
3:16
FPLC Purification of Hsp104
5:14
Representative Results for Hsp104 Purification and Assessment of Disaggregation Activity
7:04
Conclusion
在这里,我们描述一个高度活跃Hsp104,一个六聚AAA +从酵母中的蛋白质,其中夫妇ATP水解蛋白分解净化的协议。该计划利用从亲和纯化His6标签的构造 E。大肠杆菌其次,His6标签去除TEV蛋白酶,体积排阻色谱阴离子交换层析。
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