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DOI :
10.3791/3255-v
•
7:18 min
October 27th, 2011
Chapters
0:05
Title
2:04
Cell Lysis and DNA Spreading
1:21
In vivo Labeling DT40 Cells
2:53
Immunofluorescence Staining
4:31
Image Acquisition and Data Analysis
5:24
Replication Structures
6:54
Conclusion
DT40,模型脊椎动物的遗传系统,提供了强大的工具来分析蛋白质的功能。在这里,我们描述了一个简单的方法,允许在DT40细胞在单分子水平的S期DNA合成的影响参数的定性分析。
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