Name: enrichment of nk cells from human blood with the rosettesep kit from stemcell technologies
Uploaded: 2007-10-01T00:00:00
Description: Watch this Scientific Journal Video about Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies at JoVE.com

一个详细的协议，包括与每个玫瑰花九月隔离鸡尾酒，是对干细胞技术的网站 1可在网上。

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	<li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Procedures and instruction manuals. , .</li></ol>

使用这种技术孤立的细胞可进一步分为不同的人群，使用其他细胞分离试剂盒。

PBS与2％FCS和Histopaque解决方案的补充，必须到室温的过程的开始前。

enrichment of nk cells from human blood with the rosettesep kit from stemcell technologies

自然杀伤（NK）细胞的大颗粒属于先天免疫系统发挥重要的角色，在对抗癌症和感染的细胞毒性淋巴细胞，但也牵连在怀孕和移植排斥反应的早期阶段。这些细胞是外周血中，从它们可以被隔离。细胞可以分离出积极或消极的选择。我们正选择使用表面标记的抗体只对目前的利益，而细胞阴性选择感兴趣的所有细胞，但细胞中存在，我们使用有针对性的表面标记抗体鸡尾酒。后者的技术，提出了离开的抗体的利息细胞的优势，从而减少了不需要的细胞的活化或differenciation的风险。在这个视频协议，我们将演示如何通过阴性选择分离从人体血液中的NK细胞，利用干细胞技术的RosetteSep套件。该过程包括获取人外周血（下一个机构审查委员会批准的协议，以保护人类受试者），将缺席对NK细胞标记结合的抗体，鸡尾酒混合，但目前所有其他周边目前的单核细胞血液（例如，T淋巴细胞，单核细胞...).在鸡尾酒中的抗体结合向血型糖蛋白A对红细胞的抗体。因此，将所有不需要的细胞和红细胞被困联合体。然后稀释血和抗体鸡尾酒混合，是上Histopaque梯度重叠，离心。 NK细胞（&gt; 80％的纯），可以收集Histopaque和稀释后的血浆之间的接口。类似的鸡尾酒为其他的细胞群，如人类T淋巴细胞，浓缩。

Watch this Scientific Journal Video about Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies at JoVE.com

Enrichment of NK Cells from Human Blood with the RosetteSep Kit from StemCell Technologies

自然杀伤细胞是淋巴细胞的人口少。在这里，我们展示如何隔离这些细胞从人体血液中阴性选择，使用干细胞技术的试剂盒。获得的细胞是可行的和不变的抗体，因此准备了一些程序使用。

NK细胞与干细胞技术的RosetteSep套件的人体血液的富集

Natural killer (NK) cells are large granular cytotoxic lymphocytes that belong to the innate immune system and play major roles in fighting against cancer ...

Research

JoVE Journal

Biology

Purifying Plasmid DNA from Bacterial Colonies Using the Qiagen Miniprep Kit

使用Qiagen公司的小量制备试剂盒的细菌菌落纯化质粒DNA

Plasmid DNA purification from E. coli is a core technique for molecular cloning. Small scale purification (miniprep) from less than 5 ml of bacterial ...

科研

生物学

Immunohistochemistry: Paraffin Sections Using the Vectastain ABC Kit from Vector Labs

免疫组化：石蜡切片从Vector实验室使用Vectastain ABC试剂盒

Immunohistochemistry (IHC) is a valuable technique utilized to localize/visualize protein expression in a mounted tissue section using specific ...

RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit

使用Bio - Rad公司的总RNA试剂盒从Neuroprecursor细胞的RNA提取

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

从新生小鼠的肺血管内皮细胞的分离和培养

Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

代人类诱导多能干细胞从外周血中使用STEMCCA慢病毒载体

Through the ectopic expression of four transcription factors, Oct4, Klf4, Sox2 and cMyc, human somatic cells can be converted to a pluripotent state, ...

Collection, Isolation and Enrichment of Naturally Occurring Magnetotactic Bacteria from the Environment

从环境中自然产生的趋磁细菌的采集，分离和富集

Magnetotactic bacteria (MTB) are aquatic microorganisms that were first notably described in 19751 from sediment samples collected in salt marshes of ...

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

从人骨骼肌隔离血管源性多能前体

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization&#160;of MSCs have been obscured by their retrospective ...

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

A few years ago, the establishment of human induced pluripotent stem cells (iPSCs) ushered in a new era in biomedicine. Potential uses of human iPSCs ...

Isolation of Small Noncoding RNAs from Human Serum

小非编码RNA从人血清中分离

The analysis of RNA and its expression is a common feature in many laboratories. Of significance is the emergence of small RNAs like microRNAs, which are ...

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

人外周血代文化血液的产物内皮细胞

Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular ...