Name: selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation
Uploaded: 2011-12-28T12:00:00
Description: Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

“ERA - NET神经元”的框架，这项工作是由寿险公司“诺瓦利斯Taitbout”，巴黎高等DES神经科学（ENP），法新社国立德拉Recherche“的ANR - 09 - NEUR - 004”的支持由欧洲委员会第七框架计划，“基金会争取RECHERCHE Medicale”，Letten基金会和巴斯德基金会。

1。立体定向注射<ol><li>在这些实验中使用的病毒是一个艾滋病毒的复制缺陷型慢病毒载体来表达Channelrhodopsine2 - YFP（黄色荧光蛋白）融合构建一个突触子 6驱动。质粒是慷慨提供K. Deisseroth的研究组（ <a href="http://www.stanford.edu/group/dlab/optogenetics/index.html">http://www.stanford.edu/group/dlab/optogenetics/index.html</a> ）。慢病毒可以从商业渠道获得，或在实验室中根据既定协议 7 。平均载体滴度?...

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Optogenetic工具最近增加了我们对选择性的神经元的人群，包括出生于成人的嗅觉系统神经元的控制。在这里，我们描述了如何执行的慢病毒载体表达ChR2 - YFP的成人出生的神经元的具体人口的立体注射。通过仔细监测期间注射后，我们能够分析和处理出生于一个相对同质化时代的成人神经元群活动。 由于他们的低扩散蔓延，其高转导效率，其相对良好的RMS神经母细胞向性，慢?...

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selective viral transduction of adult-born olfactory neurons for chronic in vivo optogenetic stimulation

当地interneurons不断再生1-3成年啮齿动物的嗅球。在这个过程中，被称为成年神经发生，神经干细胞在侧脑室壁引起神经母细胞迁移沿喙迁徙流（RMS）达到并纳入到嗅球几毫米。为了研究不同的步骤和成人出生的神经元整合到预先存在的嗅觉电路的影响，这是必要的选择性标签和操纵的这种神经元的具体人口的活动。 optogenetic技术最近的发展提供了机遇，利用光，正是这个特定的神经元激活，而不会影响周围神经元 4,5队列。在这里，我们目前在神经母细胞的时间限制在RMS队列一个维拉利表达Channelrhodopsin2系列程序（ChR2）YFP的，在他们到达嗅球之前，成为成人出生NEurons。此外，我们将展示如何植入和校准为慢性LED 在体内刺激的ChR2表达神经元的一个缩影。

Watch this Scientific Journal Video about Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation at JoVE.com

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

成人出生在嗅球的神经元可optogenetically控制使用Channelrhodopsin2表达慢病毒注射在延髓的迁徙流和植入微型慢性photostimulation的LED。

慢性成人出生的嗅觉神经元的选择性病毒转导在体内 Optogenetic刺激

Local interneurons are continuously regenerated in the olfactory bulb of adult rodents1-3. In this process, called adult neurogenesis, neural stem cells ...

Research

JoVE Journal

Neuroscience

Selective Viral Transduction of Adult-born Olfactory Neurons for Chronic in vivo Optogenetic Stimulation

Studying the Integration of Adult-born Neurons

成人出生的神经元的整合研究

Neurogenesis occurs in adult mammalian brains in the sub-ventricular zone (SVZ) of the lateral ventricle and in the sub-granular zone (SGZ) of the ...

科研

神经科学

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

Lentivirus-mediated Genetic Manipulation and Visualization of Olfactory Sensory Neurons in vivo

嗅觉感官神经元的慢病毒介导的遗传操作和可视化在体内</em

Development of a precise olfactory circuit relies on accurate projection of olfactory sensory neuron (OSN) axons to their synaptic 
targets in the ...

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory  Neurons

闪光光解笼状化合物在嗅感觉神经元的纤毛

Photolysis of caged compounds allows the production of rapid and localized increases in the concentration of various physiologically active compounds1. ...

Fiber-optic Implantation for Chronic Optogenetic Stimulation of Brain Tissue

光纤植入慢性Optogenetic刺激脑组织

Elucidating patterns of neuronal connectivity has been a challenge for both clinical and basic neuroscience. Electrophysiology has been the gold standard ...

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Optogenetic刺激的逃避行为<em&gt;果蝇</em

A  growing number of genetically encoded tools are becoming available that allow non-invasive  manipulation of the neural activity of specific neurons in ...

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

单个锥体神经元的高保真光遗传学控制方法<em&gt;在体内</em

Optogenetic methods  have emerged as a powerful tool for elucidating neural circuit activity  underlying a diverse set of behaviors across a broad range ...

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

Whole Mount Immunolabeling of Olfactory Receptor Neurons in the Drosophila Antenna

嗅觉受体神经元在全山免疫标记<em&gt;果蝇</em&gt;天线

Odorant molecules bind to their target receptors in a precise and coordinated manner. Each receptor recognizes a specific signal and relays this ...

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

In vivo Optogenetic Stimulation of the Rodent Central Nervous System

在啮齿动物中枢神经系统的体内光遗传学刺激

The ability to probe defined neural circuits in awake, freely-moving animals with cell-type specificity, spatial precision, and high temporal resolution ...

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

新生小鼠脑侧脑室注射病毒的持续和广泛的神经元传导

With the pace of scientific advancement accelerating rapidly, new methods are needed for experimental neuroscience to quickly and easily manipulate gene ...

Optogenetic Stimulation of the Auditory Nerve

听觉神经的光遗传学刺激

Direct electrical stimulation of spiral ganglion neurons (SGNs) by cochlear implants (CIs) enables open speech comprehension in the majority of implanted ...