Name: protocols for vaginal inoculation and sample collection in the experimental mouse model of candida vaginitis
Uploaded: 2011-12-08T12:00:00
Description: Watch this Scientific Journal Video about Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis at JoVE.com

이 작품은 R01 AI32556 (NIAID, 건강의 국립 연구소)에 의해 지원되었다. 이 작품은 또한 루이지애나 백신 센터와 Regents의 루이지애나위원회 주최 감염증 연구 남부 루이지애나 연구소에 의해 부분적으로 지원되었다.

1. 칸디다 albicans와 질 접종 <ol><li> 이전 접종에 사흘은 복부를 노출하기 위해 동물을 억제하면서, 낮은 복부에 subcutaneously β - estradiol의 0.1-0.5 MG를 포함 참기름 100 μl를 주입. 사출 사이트에서 누출을 최소화하기 위해 피부에 측면 5-10mm에 대한 바늘을 전진. 아래 복부에있는 에스 트로겐의 피하 행정부는 성기 요로에 근접으로 인해이 모델에 최적입니다. 효과적인 복용이 마우스 변종,...

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	<li>Abraham, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inducible+immunity+to+Trichomonas+vaginalis+in+a+mouse+model+of+vaginal+infection.">Inducible immunity to Trichomonas vaginalis in a mouse model of vaginal infection.</a> Infect. Immun. 64, 3571-3571 (1996).</li><li>Black, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Major+histocompatibility+haplotype+does+not+impact+the+course+of+experimentally+induced+murine+vaginal+candidiasis.">Major histocompatibility haplotype does not impact the course of experimentally induced murine vaginal candidiasis.</a> Lab. Anim. Sci. 49 (6), 668-668 (1999).</li><li>Black, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+neutropenia+decreases+inflammation+associated+with+murine+vaginal+candidiasis+but+has+no+effect+on+the+course+of+infection.">Acute neutropenia decreases inflammation associated with murine vaginal candidiasis but has no effect on the course of infection.</a> Inf. Immun. 66, 1273-1273 (1998).</li><li>Black, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Increased+severity+of+Candida+vaginitis+in+BALB/c+nu/nu+mice+versus+the+parent+strain+is+not+abrogated+by+adoptive+transfer+of+T+cell+enriched+lymphocytes.">Increased severity of Candida vaginitis in BALB/c nu/nu mice versus the parent strain is not abrogated by adoptive transfer of T cell enriched lymphocytes.</a> J. Reprod. Immunol. 45, 1-1 (1999).</li><li>Buchannan, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+stromal+and+epithelial+estrogen+receptors+in+vaginal+epithelial+proliferation,+stratification,+and+cornification.">Role of stromal and epithelial estrogen receptors in vaginal epithelial proliferation, stratification, and cornification.</a> Endocrinology. 139 (10), 4345-4345 (1998).</li><li>Clemons, K. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genetic+susceptibility+of+mice+to+Candida+albicans+vaginitis+correlates+with+host+estrogen+sensitivity.">Genetic susceptibility of mice to Candida albicans vaginitis correlates with host estrogen sensitivity.</a> Infect. Immun. 72, 4878-4878 (2004).</li><li>Conrady, C. D., Halford, W. P., Carr, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Loss+of+the+type+I+interferon+pathway+increases+vulnerability+of+mice+to+genital+Herpes+simplex+virus+2+infection.">Loss of the type I interferon pathway increases vulnerability of mice to genital Herpes simplex virus 2 infection.</a> J. Virol. 85 (4), 1625-1625 (2011).</li><li>Cunha, G. R., Cooke, P. S., Kurita, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+estromal-epithelial+interaction+in+hormonal+responses.">Role of estromal-epithelial interaction in hormonal responses.</a> Arch Histol Cytol. 67 (5), 417-417 (2004).</li><li>Enjalbert, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+multifunctional,+synthetic+Caussia+princeps+luciferase+reporter+for+live+imaging+of+Candida+albicans+infections.">A multifunctional, synthetic Caussia princeps luciferase reporter for live imaging of Candida albicans infections.</a> 77 (11), 4847-4847 (2009).</li><li>Feinen, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Critical+role+of+Th17+responses+in+a+murine+model+of+Neisseria+gonorrhoeae+genital+infection.">Critical role of Th17 responses in a murine model of Neisseria gonorrhoeae genital infection.</a> Mucosal Immunol. 3 (3), 312-312 (2010).</li><li>Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinct+protective+host+defenses+against+oral+and+vaginal+candidiasis.">Distinct protective host defenses against oral and vaginal candidiasis.</a> Med. Mycol. 40, 359-359 (2002).</li><li>Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+intravaginal+live+Candida+challenge+in+humans+leads+to+new+hypotheses+for+the+immunopathogenesis+of+vulvovaginal+candidiasis.">An intravaginal live Candida challenge in humans leads to new hypotheses for the immunopathogenesis of vulvovaginal candidiasis.</a> Infect. Immun. 72, 2939-2939 (2004).</li><li>Fidel, P. L., Cutright, J. L., Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficacy+of+D0870+treatment+of+experimental+Candida+vaginitis.">Efficacy of D0870 treatment of experimental Candida vaginitis.</a> Antimicrob. Agents. Chemother. 41, 1455-1455 (1997).</li><li>Fidel, P. L., Cutright, J. L., Steele, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+Reproductive+hormones+on+experimental+vaginal+candidiasis.">Effects of Reproductive hormones on experimental vaginal candidiasis.</a> Infect. Immun. 68, 651-651 (2000).</li><li>Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+murine+model+of+Candida+glabrata+vaginitis.">A murine model of Candida glabrata vaginitis.</a> J. Inf. Dis. 173, 425-425 (1996).</li><li>Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+vaginal+cell+populations+during+experimental+vaginal+candidiasis.">Analysis of vaginal cell populations during experimental vaginal candidiasis.</a> Inf. Immun. 67, 3135-3135 (1999).</li><li>Fidel, P. L., Lynch, M. E., Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Candida-specific+cell-mediated+immunity+is+demonstrable+in+mice+with+experimental+vaginal+candidiasis.">Candida-specific cell-mediated immunity is demonstrable in mice with experimental vaginal candidiasis.</a> Infect. Immun. 61, 1990-1990 (1993).</li><li>Fidel, P. L., Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunopathogenesis+of+recurrent+vulvovaginal+candidiasis.">Immunopathogenesis of recurrent vulvovaginal candidiasis.</a> Clin. Microbiol. Rev. 9. 9, 335-335 (1996).</li><li>Fidel, P. L., Sobel, J. D., Zak, O., Sande, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Murine Models of Candida Vaginal Infections, In Experimental models in antimicrobial chemotherapy. , 741-748 (1999).</li><li>Fidel, P. L., Vazquez, J. A., Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Candida+glabrata:+Review+of+epidemiology,+pathogenesis,+and+clinical+disease+with+comparison+to+C.+albicans.">Candida glabrata: Review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans.</a> Clin. Microbiol. Rev. 12, 80-80 (1999).</li><li>Fulurija, A., Ashman, R. B., Papadimitriou, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutrophil+depletion+increases+susceptibility+to+systemic+and+vaginal+candidiasis+in+mice,+and+reveals+differences+between+brain+and+kidney+in+mechanisms+of+host+resistance.">Neutrophil depletion increases susceptibility to systemic and vaginal candidiasis in mice, and reveals differences between brain and kidney in mechanisms of host resistance.</a> Microbiology. 142, 3487-3487 (1996).</li><li>Gill, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=NK+cells+require+type+I+IFN+receptor+for+antiviral+responses+during+genital+HSV-2+infection.">NK cells require type I IFN receptor for antiviral responses during genital HSV-2 infection.</a> Cell Immunol. 269 (1), 29-29 (2011).</li><li>Hamad, M., Abu-Elteen, K. H., Ghaleb, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Estrogen-dependent+induction+of+persistent+vaginal+candidosis+in+naive+mice.">Estrogen-dependent induction of persistent vaginal candidosis in naive mice.</a> Cell. Immunol. 47 (7), 304-304 (2004).</li><li>Hamad, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Utility+of+the+oestrogen-dependent+vaginal+candidosis+murine+model+in+evaluating+the+efficacy+of+various+therapies+against+vaginal+Candida+albicans+infection.">Utility of the oestrogen-dependent vaginal candidosis murine model in evaluating the efficacy of various therapies against vaginal Candida albicans infection.</a> Mycoses. 49 (2), 104-104 (2006).</li><li>LeBlanc, D. M., Barousse, M. M., Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+role+for+dendritic+cells+in+immunoregulation+during+experimental+vaginal+candidiasis.">A role for dendritic cells in immunoregulation during experimental vaginal candidiasis.</a> Infect. Immun. 74, 3213-3213 (2006).</li><li>McGrory, T., Garber, G. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+intravaginal+infection+with+Trichomonas+vaginalis+and+role+of+Lactobacillus+acidophilus+in+sustaining+infection.">Mouse intravaginal infection with Trichomonas vaginalis and role of Lactobacillus acidophilus in sustaining infection.</a> Infect. Immun. 60, 2375-2379 (1992).</li><li>Naglik, J. R., Fidel, P. L., Odds, F. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+mucosal+Candida+infection.">Animal models of mucosal Candida infection.</a> FEMS. Microbiol. Lett. 283 (2), 129-129 (2008).</li><li>Nomanbhoy, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vaginal+and+oral+epithelial+cell+anti-Candida+activity.">Vaginal and oral epithelial cell anti-Candida activity.</a> Inf. Immun. 70, 7081-7081 (2002).</li><li>Pietrella, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+beta-glucan-conjugate+vaccine+and+anti-beta-glucan+antibodies+are+effective+against+murine+vaginal+candidiasis+as+assessed+by+a+novel+in+vivo+imaging+technique.">A beta-glucan-conjugate vaccine and anti-beta-glucan antibodies are effective against murine vaginal candidiasis as assessed by a novel in vivo imaging technique.</a> Vaccine. 28 (7), 1717-1717 (2010).</li><li>Redondo-Lopez, V., Cook, R. N., Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emerging+role+of+Lactobacilli+in+the+control+and+maintenance+of+the+vaginal+bacterial+microflora.">Emerging role of Lactobacilli in the control and maintenance of the vaginal bacterial microflora.</a> Rev Infect Dis. 12 (5), 856-856 (1990).</li><li>Saavedra, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+production+of+chemokines+during+experimental+vaginal+candidiasis.">Local production of chemokines during experimental vaginal candidiasis.</a> Inf. Immun. 67, 5820-5820 (1999).</li><li>Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogenesis+and+epidemiology+of+vulvovaginal+candidiasis.">Pathogenesis and epidemiology of vulvovaginal candidiasis.</a> Ann. N. Y. Acad. Sci. 544, 547-547 (1988).</li><li>Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathogenesis+and+treatment+of+recurrent+vulvovaginal+candidiasis.">Pathogenesis and treatment of recurrent vulvovaginal candidiasis.</a> Clin. Infect. Dis. 14, S148-S153 (1992).</li><li>Sobel, J. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vulvovaginal+candidiasis:+Epidemiologic,+diagnostic,+and+therapeutic+considerations.">Vulvovaginal candidiasis: Epidemiologic, diagnostic, and therapeutic considerations.</a> Am. J. Obstet. Gynecol. 178 (2), 203-203 (1998).</li><li>Song, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+and+humoral+immune+responses+against+primary+and+repeat+Neisseria+gonorrhoeae+genital+tract+infections+of+17&#946;-estradiol-treated+mice.">Local and humoral immune responses against primary and repeat Neisseria gonorrhoeae genital tract infections of 17&#946;-estradiol-treated mice.</a> Vaccine. 26, 5741-5741 (2008).</li><li>Taylor, B. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+virulence+of+Candida+albicans+isolates+causing+mucosal+infections+in+people+infected+with+the+human+immunodeficiency+virus.">In vivo virulence of Candida albicans isolates causing mucosal infections in people infected with the human immunodeficiency virus.</a> J. Infect. Dis. 182, 955-955 (2000).</li><li>Taylor, B. N., Saavedra, M., Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Local+Th1/Th2+cytokine+production+during+experimental+vaginal+candidiasis.">Local Th1/Th2 cytokine production during experimental vaginal candidiasis.</a> Med. Mycol. 38, 419-419 (2000).</li><li>Tirabassi, R. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+mucosal+vaccination+approach+for+herpes+simplex+virus+type+2.">A mucosal vaccination approach for herpes simplex virus type 2.</a> Vaccine. 29 (5), 1090-1090 (2011).</li><li>Broeck, W. V. a. n. d. e. n., Derore, A., Simoens, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anatomy+and+nomenclature+of+murine+lymph+nodes:+descriptive+and+nomenclatory+standardization+in+BALB/cAnNCrl+mice.">Anatomy and nomenclature of murine lymph nodes: descriptive and nomenclatory standardization in BALB/cAnNCrl mice.</a> J. Immunol. Methods. 312 (1-2), 12-12 (2006).</li><li>Wormley, F. L., Chaiban, J., Fidel, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+adhesion+molecule+and+lymphocyte+activation+marker+expression+during+experimental+vaginal+candidiasis.">Cell adhesion molecule and lymphocyte activation marker expression during experimental vaginal candidiasis.</a> J. Immunol. Methods. 69, 5072-5072 (2001).</li><li>Yano, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epithelial+cell-derived+S100+calcium-binding+proteins+as+key+mediators+in+the+hallmark+acute+neutrophil+response+during+Candida+vaginitis.">Epithelial cell-derived S100 calcium-binding proteins as key mediators in the hallmark acute neutrophil response during Candida vaginitis.</a> Infect. Immun. 78 (12), 5126-5126 (2010).</li></ol>

칸디다 질염의 실험 마우스 모델, 설립 역사적 3,4,11,13,16,17,19,21,24 antifungal 요법을 테스트뿐만 아니라 칸디다에 점막 호스트 응답을 연구하기 위해 지난 몇 십년에 사용되고 있습니다 25,37. 프로토콜은 여기 효율적이고 덜 노동 집약적인 방법 통합, 그리고 칸디다 질염의 가장 최적화된 모델 시스템 중 하나가 날짜 설명한 것으로 나타날 제시. 이 기술은...

<table border="1"><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td><td> 댓글 </td></tr><tr><td> 여자 CBA / J 마우스 </td><td> 찰스 리버 연구소 </td><td> 01C38 </td><td> 나이 5-6주 </td></tr><tr><td> 칸디다 albicans (3153A) </td><td> 병원성 곰팡이 국립 수집, 영국 </td><td> NCPF3153 </td><td></td></tr><tr><td> 향유 </td><td> 시그마 - 올드 리치 </td><td> S3547 </td><td> 사용하기 전에 미리 소독을 할 필요가 없다 </td></tr><tr><td> Β - estradiol 17 - valerate </td><td> 시그마 - 올드 리치 </td><td> E1631 </td><td> 참깨 기름에 0.1 - 0.5mg </td></tr><tr><td> Phytone 펩톤 </td><td> Becton 디킨슨 </td><td> 211,906 </td><td> 0.1 % 포도당과 보충 </td></tr><tr><td> Trypan 푸른 솔루션 </td><td> 시그마 - 올드 리치 </td><td> T8154 </td><td></td></tr><tr><td> 덱스 트로 오스 Sabouraud 한천 </td><td> Becton 딕inson </td><td> 211,584 </td><td></td></tr><tr><td> Collagenase 유형 IV </td><td> 시그마 - 올드 리치 </td><td> C5138 </td><td> 0.25 % </td></tr><tr><td> Dispase </td><td> Invitrogen </td><td> 17105-041 </td><td> 1.7 U / ML </td></tr><tr><td> 와이어 메쉬 스크린 </td><td> TWP </td><td> 060X060S0065W36T </td><td> 번호 60 메쉬, 스테인레스 </td></tr><tr><td> 행크스 &#39;균형 소금 솔루션 </td><td> Invitrogen </td><td> 24020-117 </td><td></td></tr><tr><td> CytoPrep 정착액 </td><td> 피셔 과학 </td><td> 12-570-10 </td><td> 얼룩 슬라이드를 보존 </td></tr><tr><td> Papanicolaou는 EA - 65 얼룩 </td><td> EMD 화학 </td><td> 7054X 85 </td><td></td></tr><tr><td> Papanicolaou는 OG - 6 얼룩 </td><td> EMD 화학 </td><td> 7052X 85 </td><td></td></tr><tr><td> 해리스 &#39;명반의 hematoxylin </td><td> EMD 화학 </td><td> 638A - 85 </td><td></td></tr><tr><td> Isoflurane </td><td> 박스터 헬스케어 </td><td> NDC 10019-773-60 </td><td> isoflurane 기화기 또는 드롭 시스템에서 사용되는 마취 챔버 폐쇄 </td></tr></table>

protocols for vaginal inoculation and sample collection in the experimental mouse model of candida vaginitis

칸디다 종에 의한 Vulvovaginal 칸디다 증 (VVC)은, 그들의 생식 년 18,32-34 동안 달리 건강한 여성의 약 75 %에 영향을 미치는 낮은 여성의 성기 요로의 곰팡이 감염입니다. Predisposing 요인 항생제 사용, 통제 당뇨병 방해 생식 호르몬 수준에 임신으로 인해, 경구 피임약이나 호르몬 대체 요법 33,34을 포함합니다. 연간 세 개 이상의 에피소드로 정의된 재발성 VVC (RVVC)는없고 predisposing 요인 33 여성의 8 %를 별도의 다섯 영향을 미칩니다. VVC의 실험 마우스 모델은 설립 pathogenesis과 칸디다 3,4,11,16,17,19,21,25,37에 점막 호스트 응답을 연구하는 데 사용되었습니다. 이 모델은 또한 생체내 13,24 잠재적인 antifungal 치료를 테스트하기 위해 고용되었습니다. 모델 동물에 대한 선택 pseudoestrus의 상태로 유지합니다imal 칸디다 식민 / 감염 6,14,23. 이러한 조건에서, 주사 동물 개월 주 동안 질 곰팡이 부담을 감지합니다. 과거 연구는 면역 및 생리 특성 3,16,21에 상대적인 동물 모델과 인간 사이의 감염이 매우 높은 병렬을 보여줍니다. 차이점은, 그러나, 정상적인 질 식물로 칸디다의 부족과 생쥐의 중립 질 산도를 포함합니다. 여기에서 우리는 질 접종, 질 표본의 신속한 수집, 질 곰팡이 부담 평가 및 세포 추출 / 격리에 대한 조직 준비를 포함하는 마우스 질염 모델의 주요 방법의 일련 보여줍니다. 이것은 질 세척 유체, 곰팡이 부담, 그리고 배수 림프절의 백혈구의 수율의 성분에 대한 대표적인 결과는 다음입니다. anesthetics의 사용으로 세척 샘플의 길이 평가를 위해 같은 생쥐에 여러 시간 지점에서 수령 가능감염 / 식민지. 또한,이 모델은 정의된 호스트 조건 하에서 면역 연구를 허용, 감염을 시작하는 데 아무런 immunosuppressive 요원을 필요하지 않습니다. 마지막으로, 모델 및 각 기술은 여기에 잠재적으로 낮은 여성의 성기 요로 (바이러스성, 기생, 세균)과 각 지역 또는 체계 호스트 방어의 다른 감염증을 조사하는 방법론의 사용에 상승을 줄 수 소개했다.

Watch this Scientific Journal Video about Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis at JoVE.com

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

평가에 사용되는 주요 기술<em&gt; 칸디다</em실험 동물 모델에서&gt; 질염이 설명되어 있습니다. 방법은 허리 림프절을 배수의 질 표본 및 lymphocytes의 급속한 컬렉션을하실 수 있습니다. 이 기술은 여성의 낮은 성기 요로에있는 다른 질병의 마우스 모델에 상승을 줄 수 있습니다.

의 실험 마우스 모델에서 질 접종 및 샘플 수집을위한 프로토콜 칸디다 질염

Vulvovaginal candidiasis (VVC), caused by Candida species, is a fungal infection of the lower female genital tract that affects approximately 75% of ...

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Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

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