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DOI :
10.3791/3416-v
February 20th, 2012
Chapters
0:05
Title
1:31
Virus Production
3:53
Viral Transduction
5:47
Reprogramming
7:24
Isolation of Human iPS Cell Colonies
11:14
Representative Results: Examining Transduction Efficiency and Characterizing Reprogrammed Cells
12:58
Conclusion
我们提出了一个高效的人体细胞重编程为人类诱导多能干细胞(hiPSC)用OCT3 / 4,SOX2,KLF4和c-myc(OSKM)的逆转录病毒载体编码和识别正确地重新编程,现场染色hiPSC协议茶1-81抗体。
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