Name: rnai-mediated gene knockdown and in vivo diuresis assay in adult female aedes aegypti mosquitoes
Uploaded: 2012-07-14T14:00:00
Description: Watch this Scientific Journal Video about RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes at JoVE.com

作者感谢她的这个协议的批评维多利亚木匠。

第一部分- RNAi介导的基因敲除成年埃及伊蚊蚊。实验概述， 见图1。 1。双链RNA的合成<ol><li>对利益和控制的dsRNA基因合成的双链RNA。注：我们建议发展中国家引物的PCR片段范围在300至500个碱基对位于特定基因2月底3&#39;和T7引物序列在5&#39;端（5&#39;-TAA TAC GAC中TCA的CTA重视。豪GG-3&#39;）。片段的唯一性，应确认BLASTN程序分析3。 </li><li>使用的Ambio...

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RNAi的协议，用于已开发在Raikhel亚历山大实验室在加州河滨大学6,7和类似由加弗和Dimopoulos 4发表了一份协议。这个视频协议中的实验方法可以用来研究有关的基因在昆虫体内设置利尿。作为一个简单的“利尿模型系统，吸引昆虫，马氏管，排泄器官几代科研人员的利益。这个器官是涉及通关8和果蝇的工作已确定在马氏管生理9,10，现在可以在其他昆虫...

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rnai-mediated gene knockdown and in vivo diuresis assay in adult female aedes aegypti mosquitoes

这个视频协议演示了一个有效的技术击倒在昆虫特定基因，并进行一种新的生物活性来衡量排泄率。这种方法可以用于获取更好地了解昆虫的利尿过程，是在利尿吸血节肢动物，能够占用大量的液体，在一个单一的血粉的研究，特别是有用的。 这RNAi介导的基因敲除结合在体内利尿法是由汉森实验室研究RNAi介导水通道蛋白基因埃及伊蚊利尿1击倒的影响。 该协议是在设置两部分：第一个示范说明如何构建一个简单的蚊子喷射装置及如何准备和注入的蚊子胸部RNAi介导的基因敲除的dsRNA。第二个示范说明如何确定在使用延命蚊子的排泄率。

Watch this Scientific Journal Video about RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes at JoVE.com

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

在这个协议中，我们结合RNAi介导的基因沉默<em&gt;在体内</em&gt;利尿法研究感兴趣的基因的影响击倒蚊虫液的排泄。

RNAi介导的基因敲除和<em&gt;在体内</em在成年女性利尿含量<em&gt;埃及伊蚊</em&gt;蚊子

This video protocol demonstrates an effective technique to knockdown a particular gene in an insect and conduct a novel bioassay to measure excretion ...

Research

JoVE Journal

Biology

RNAi-mediated Gene Knockdown and In Vivo Diuresis Assay in Adult Female Aedes aegypti Mosquitoes

Injection of dsRNA into Female A. aegypti Mosquitos

女答：埃及伊蚊蚊的dsRNA注入

Reverse genetic approaches have proven extremely useful for determining  which genes underly resistance to vector pathogens in mosquitoes.  This video ...

科研

生物学

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes

埃及斑蚊胚胎显微注射获得转基因蚊子

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue ...

Protocol for Dengue Infections in Mosquitoes (A. aegypti) and Infection Phenotype Determination

Protocol for Dengue Infections in Mosquitoes A. aegypti and Infection Phenotype Determination

在蚊子（埃及斑蚊）感染引起的表型测定为登革热感染病例的议定书“

The purpose of this procedure is to infect the Aedes mosquito with dengue virus in a laboratory condition and examine the infection level and dynamic of ...

Dissection of Midgut and Salivary Glands from Ae. aegypti Mosquitoes

从AE肠和唾液腺的解剖。埃及伊蚊蚊

The mosquito midgut and salivary glands are key entry and exit points for pathogens such as Plasmodium parasites and Dengue viruses. This video protocol ...

RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus

RNAi Mediated Gene Knockdown and Transgenesis by Microinjection in the Necromenic Nematode Pristionchus pacificus

通过显微注射介导的RNAi Necromenic线虫基因敲除和转基因 Pristionchus pacificus</em

Although it is increasingly affordable for emerging model organisms to obtain completely sequenced genomes, further in-depth gene function and expression ...

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

作为一种工具来隔离核糖体的束缚多肽SECM逮捕序列

Extensive research has provided ample evidences suggesting that protein folding in the cell is a co-translational process1-5. However, the exact pathway ...

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

In Vivo SiRNA Transfection and Gene Knockdown in Spinal Cord via Rapid Noninvasive Lumbar Intrathecal Injections in Mice

体内转染和基因敲除在脊髓通过快速无创腰椎鞘内注射小鼠

This report describes a step-by-step guide to the technique of acute intrathecal needle injections in a noninvasive manner, i.e. independent of catheter ...

Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

肥胖的身体器官培养体系中蚊，一个向量 Zika 病毒

The insect fat body plays a central role in insect metabolism and nutrient storage, mirroring functions of the liver and fat tissue in vertebrates. Insect ...

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

Maintaining Aedes aegypti Mosquitoes Infected with Wolbachia

维护伊蚊蚊蚊子感染沃尔巴克

Aedes aegypti mosquitoes experimentally infected with Wolbachia are being utilized in programs to control the spread of arboviruses such as dengue, ...

Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates (EAgaL Plates)

Improved Fecundity and Fertility Assay for Aedes aegypti using 24 Well Tissue Culture Plates EAgaL Plates

使用 24 个井组织培养板 （EAgaL 板） 改进 了伊蚊 的丰度和生育率测定

Mosquitoes represent a significant public health problem as vectors of various pathogens. For those studies that require an assessment of mosquito fitness ...