Name: labeling stem cells with ferumoxytol, an fda-approved iron oxide nanoparticle
Uploaded: 2011-11-04T13:00:00
Description: Watch this Scientific Journal Video about Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle at JoVE.com

这项工作是支持由国家关节炎，肌肉骨骼及皮肤疾病研究所授予：​​3R01AR054458 - 02S2。

1。第1天 1）板细胞<ol><li>板HMSC T75烧瓶在一个汇合的80％至少18-24小时前标签。备用船只的指令，请参考表1。 </li></ol> 2。第2天 2）准备标签的解决方案。这种准备将标签一（1）T75烧瓶中铁400微克/毫升的浓度在80％汇合。备用船只的指令，请参考表1。 <ol><li>创建一个解决方案（解决方案1），1毫升的无血清培养基和93.1μLferumoxytol（股票：30?...

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提高疗效的干细胞engraftments是再生医学的进步是至关重要的。一个在体内的干细胞的非侵入性可视化技术，大大增强了我们的理解能力成功植入成果的机制，导致。磁性标签的议员，如我们已经证明过程的可视化，可在体内的干细胞与磁共振成像跟踪。以前的磁标记干细胞被移植到靶组织，已可视化与磁共振成像 7周。长期的标签和纵向成像是可能的， 因为 8的氧化?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td> D - MEM高糖</td><td>西格玛</td><td> D5648 </td><td>或其他所需的干细胞系用于基础培养基</td></tr><tr><td> D - PBS（ 钙离子，镁+免费） </td><td> GIBCO公司</td><td> 14190-144 </td><td></td></tr><tr><td>胰蛋白酶EDTA 0.05％ </td><td> Invitrogen公司</td><td> 25300-120 </td><td></td></tr><tr><td>胎牛血清（FBS） </td><td> Hyclone公司</td><td> SH30071.03 </td><td></td></tr><tr><td> Ferumoxytol （Feraheme） </td><td> AMAG </td><td> 59338-0775-​​01 </td><td></td></tr><tr><td>硫酸鱼精蛋白</td><td> APP的医药。 </td><td> 22930 </td><td></td></tr>
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labeling stem cells with ferumoxytol, an fda-approved iron oxide nanoparticle

干细胞为基础的疗法提供了显著的再生医学领域潜力。然而，仍有许多工作需要了解有关在体内移植的细胞动力学。一种非侵入性的方法来重复监视体内移植的干细胞，将允许调查人员直接监控干细胞移植，并确定成功或不成功的植入结果。 广泛的干细胞仍然是无数的应用研究。此协议侧重于3种不同的干细胞群：人胚肾293（HEK293）细胞，人骨髓间充质干细胞（HMSC）和诱导多能干细胞（iPS）细胞。 HEK 293细胞来自人类胚胎肾细​​胞在培养与剪切的腺病毒5 DNA。这些细胞被广泛用于研究，因为它们很容易培养，生长迅速，而且很容易转。成人骨髓中发现的hMSCs。这些细胞的CAn是未分化的细胞复制，同时保持多能或潜在的分化成的细胞命运的数量有限。的hMSCs可以分化为间充质组织，包括成骨细胞，脂肪细胞，软骨，肌腱，肌肉，骨髓基质谱系。 iPS细胞的基因重新编​​程成年细胞已被修改，以表达基因和类似胚胎干细胞的定义属性的因素。这些细胞是多能干细胞的意义，他们有能力分化成所有细胞谱系1。双方的hMSCs和iPS细胞已经证明体内组织的再生能力。 磁共振（MR）成像与超顺磁性氧化铁（SPIO）纳米颗粒细胞标签的使用已被证明有效的，在体内的干细胞，由于附近的显微解剖决议跟踪，更长的血液半衰期，允许纵向成像和高灵敏度Ÿ纳米粒子的超顺磁性氧化铁MR成像提供细胞检测2-4。此外，SPIOs使用MR成像是临床上的翻译。与右旋糖酐，carboxydextran或淀粉表面涂层服务包含从血浆成分的生物活性铁芯的氧化铁核心组成SPIOs。这些代理商创造局部磁场的不均匀性，导致一个下降T2加权MR图像5信号。不幸的是，SPIOs不再生产。第二代超小型SPIOs（USPIO），但是，提供一个可行的选择。 Ferumoxytol（FerahemeTM）是一种非化学计量的磁铁矿由缩合葡萄糖山梨醇carboxymethylether大衣包围的核心组成之一USPIO。 ferumoxytol胶体，粒径17-30纳米光散射确定。分子量为750 kDa的，2T磁共振成像领域的弛豫常数为58.609毫米1秒1的实力 4 。 Ferumoxytol最近被FDA批准一个是一个在与肾功能衰竭6患者的补铁治疗缺铁。本集团已应用在“关闭标签”细胞标记应用程序的使用此代理。我们的技术演示ferumoxytol显著MR MR图像标记细胞信号的影响，导致干细胞的有效标签。这种技术可应用于非侵入性的干细胞疗法在临床前和临床设置监测。

Watch this Scientific Journal Video about Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle at JoVE.com

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

我们描述一个标签和跟踪与FDA批准的，超顺磁性氧化铁（SPIO），ferumoxytol（Feraheme）的干细胞的技术。这种细胞成像技术，利用磁共振（MR）成像的可视化，易于成功或不成功的患者干细胞engraftments的长期监测和诊断。

Ferumoxytol，一个FDA批准的氧化铁纳米粒子标记干细胞

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