Name: labeling stem cells with ferumoxytol, an fda-approved iron oxide nanoparticle
Uploaded: 2011-11-04T13:00:00
Description: Watch this Scientific Journal Video about Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle at JoVE.com

3R01AR054458 02S2: इस काम के नेशनल इंस्टीट्यूट ऑफ संधिशोथ और Musculoskeletal और त्वचा रोग से अनुदान द्वारा समर्थित किया गया था.

1. दिवस 1 1) प्लेट कोशिकाओं <ol><li> एक confluency 80 के% T75 फ्लास्क में प्लेट कम से कम 18-24 घंटे लेबलिंग पहले hMSC. वैकल्पिक जहाजों के लिए अनुदेश के लिए तालिका 1 देखें. </li></ol> 2. 2 दिन 2) लेबलिंग समाधान तैय?...

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स्टेम सेल engraftments की प्रभावकारिता में सुधार पुनर्योजी चिकित्सा की उन्नति के लिए महत्वपूर्ण है. Vivo में स्टेम कोशिकाओं के लिए एक गैर इनवेसिव दृश्य तकनीक काफी हमारे तंत्र है कि सफल engraftment के परिणामों के लि?...

<table border="1"><tbody><tr><td> अभिकर्मक के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> डी सदस्य उच्च ग्लूकोज </td><td> सिग्मा </td><td> D5648 </td><td> मध्यम या वांछित स्टेम सेल लाइन के लिए इस्तेमाल किया जा के लिए अन्य आधार </td></tr><tr><td> डी पीबीएस (Ca + +, मिलीग्राम + मुक्त +) </td><td> GIBCO </td><td> 14190-144 </td><td></td></tr><tr><td> 0.05% trypsin - EDTA </td><td> Invitrogen </td><td> 25300-120 </td><td></td></tr><tr><td> भ्रूण गोजातीय सीरम (FBS) </td><td> Hyclone </td><td> SH30071.03 </td><td></td></tr><tr><td> Ferumoxytol (Feraheme) </td><td> AMAG </td><td> 59338-0775-01 </td><td></td></tr><tr><td> Protamine सल्फेट </td><td> एपीपी फार्म. </td><td> 22930 </td><td></td></tr>
</tbody>
</table>

labeling stem cells with ferumoxytol, an fda-approved iron oxide nanoparticle

Stem cell based therapies offer significant potential for the field of regenerative medicine. However, much remains to be understood regarding the in vivo kinetics of transplanted cells. A non-invasive method to repetitively monitor transplanted stem cells in vivo would allow investigators to directly monitor stem cell transplants and identify successful or unsuccessful engraftment outcomes.
A wide range of stem cells continues to be investigated for countless applications. This protocol focuses on 3 different stem cell populations: human embryonic kidney 293 (HEK293) cells, human mesenchymal stem cells (hMSC) and induced pluripotent stem (iPS) cells. HEK 293 cells are derived from human embryonic kidney cells grown in culture with sheared adenovirus 5 DNA. These cells are widely used in research because they are easily cultured, grow quickly and are easily transfected. hMSCs are found in adult marrow. These cells can be replicated as undifferentiated cells while maintaining multipotency or the potential to differentiate into a limited number of cell fates. hMSCs can differentiate to lineages of mesenchymal tissues, including osteoblasts, adipocytes, chondrocytes, tendon, muscle, and marrow stroma. iPS cells are genetically reprogrammed adult cells that have been modified to express genes and factors similar to defining properties of embryonic stem cells. These cells are pluripotent meaning they have the capacity to differentiate into all cell lineages 1. Both hMSCs and iPS cells have demonstrated tissue regenerative capacity in-vivo.
Magnetic resonance (MR) imaging together with the use of superparamagnetic iron oxide (SPIO) nanoparticle cell labels have proven effective for in vivo tracking of stem cells due to the near microscopic anatomical resolution, a longer blood half-life that permits longitudinal imaging and the high sensitivity for cell detection provided by MR imaging of SPIO nanoparticles 2-4. In addition, MR imaging with the use of SPIOs is clinically translatable. SPIOs are composed of an iron oxide core with a dextran, carboxydextran or starch surface coat that serves to contain the bioreactive iron core from plasma components. These agents create local magnetic field inhomogeneities that lead to a decreased signal on T2-weighted MR images 5. Unfortunately, SPIOs are no longer being manufactured. Second generation, ultrasmall SPIOs (USPIO), however, offer a viable alternative. Ferumoxytol (FerahemeTM) is one USPIO composed of a non-stoichiometric magnetite core surrounded by a polyglucose sorbitol carboxymethylether coat. The colloidal, particle size of ferumoxytol is 17-30 nm as determined by light scattering. The molecular weight is 750 kDa, and the relaxivity constant at 2T MRI field is 58.609 mM-1 sec-1 strength4. Ferumoxytol was recently FDA-approved as an iron supplement for treatment of iron deficiency in patients with renal failure 6. Our group has applied this agent in an &ldquo;off label&rdquo; use for cell labeling applications. Our technique demonstrates efficient labeling of stem cells with ferumoxytol that leads to significant MR signal effects of labeled cells on MR images. This technique may be applied for non-invasive monitoring of stem cell therapies in pre-clinical and clinical settings.

Watch this Scientific Journal Video about Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle at JoVE.com

Labeling Stem Cells with Ferumoxytol, an FDA-Approved Iron Oxide Nanoparticle

हम लेबलिंग और एफडीए को मंजूरी दी, superparamagnetic लोहे (SPIO) ऑक्साइड, ferumoxytol (Feraheme) के साथ स्टेम सेल पर नज़र रखने के लिए एक तकनीक का वर्णन. यह सेलुलर इमेजिंग तकनीक है कि दृश्य के लिए चुंबकीय अनुनाद इमेजिंग (एमआर) का इस्तेमाल करता है, दीर्घकालिक और रोगियों में सफल हो या असफल स्टेम सेल engraftments की निगरानी और निदान के लिए आसानी से पहुँचा जा सकता है.

Ferumoxytol, एक एफडीए स्वीकृत आयरन आक्साइड nanoparticle के साथ स्टेम सेल लेबल

Stem cell based therapies offer significant potential for the field of regenerative medicine. However, much remains to be understood regarding the in vivo ...

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Patients with cerebral metastasis of carcinomas have a poor prognosis. However, the process at the metastatic site has barely been investigated, in ...

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अग्नाशय के कैंसर नई उपचार रणनीतियों के विकास के लिए सेल के लक्षण स्टेम का अध्ययन

Pancreatic ductal adenocarcinoma (PDAC) contains a subset of exclusively tumorigenic cancer stem cells (CSCs) which have been shown to drive tumor ...

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सिकल सेल रोग के साथ रोगियों के लिए सतत मैनुअल एक्सचेंज ट्रांसफ्यूजन: से बचने के लिए आयरन अधिभार एक कारगर तरीका

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संश्लेषण, लक्षण वर्णन, और एक्स्ट्रापल्मोनरी तपेदिक का पता लगाने के लिए सुपरपैरामैग्नेटिक आयरन ऑक्साइड नैनोप्रोब्स का आवेदन

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ट्रैकिंग सुपरपैरामैग्नेटिक आयरन ऑक्साइड लेबल मेसेनचिमल स्टेम सेल एक दर्दनाक मस्तिष्क चोट Murine मॉडल में Intranasal वितरण के बाद एमआरआई का उपयोग कर

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