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22.9K Views
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10:25 min
January 26th, 2012
DOI :
10.3791/3485-v
Chapters
0:05
Title
1:42
Tissue Dissection, Post-fixation, and Sectioning
5:37
Immunostaining: Floating Section Staining for Plantar Punch Tissue
7:17
Immunostaining: On-slide Staining for Limb Bone Sections
8:38
Epifluorescence and Confocal Imaging of Immunostained Plantar Punch and Bone Tissue
Summary
末梢感觉神经纤维亚型的免疫细胞化学鉴定(和蛋白表达其中的检测)底层外围感觉的分子机制的理解是关键。在这里,我们描述外设/内脏组织样本,特定的免疫外周感觉神经纤维,如皮肤和四肢骨骼的制备方法。
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