Name: large-scale recording of neurons by movable silicon probes in behaving rodents
Uploaded: 2012-03-04T14:00:00
Description: Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Marie Curie International Outgoing Fellowship (Europæiske Unions FP/2007-2013 tilskudsaftalerne # 221834 og 254780), JD McDonnell Foundation, NSF Grant SBE 0542013, National Institutes of Health Grant NS034994, National Institute of Mental Health Grant MH5467 og Howard Hughes Medical Institute (Janelia Farm Research Campus tilskud).

1. Byggeriet af Microdrive Alle drev er fremstillet af de samme elementer: en bevægelig del, som bærer elektrode og en fast del, som er forankret til kraniet. En ideel mikrodrev tillader glat, men længe nok vandring elektroden i flere små trin, er robust nok til at forhindre utilsigtet bevægelse af elektroden, let at manipulere af eksperimentatoren uden at interferere med dyrets adfærd, lille i størrelse og let i vægt. Som et resultat af disse konkurrerende krav. Forskellige drev passe...

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Denne film illustrerer implantation proceduren for silicium sonder til kroniske store optagelser i opfører rotten. Kritiske skridt til at sikre kvalitet optagelser af neuronal aktivitet skyldes skrøbeligheden af ​​både biologisk (hjernevæv) og tekniske (silicium sonde) materialer. Særlig omhu bør tages under håndtering af proben for at undgå kontakt af halsene med nogen afstand &quot;hård&quot; overflade (for eksempel ville skafterne bryde, hvis man forsøgte at implantere dem i hjernen uden at fjerne dura)...

<table border="1"><tbody><tr><td> Navn </td><td> Type </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer </td></tr><tr><td> Silicon sonde Buzsaki32, 4 kalveskanker x 8 sider. Emballage: fleksibel polyamid kabel </td><td> Materiale </td><td> NeuroNexus </td><td> Probe: buzsaki32 Emballage: HC32 </td><td> Optagelse sonde </td></tr><tr><td> Runde messingskrue, 00-90 x 1/2 Runde Messing Skruer </td><td> Materiale </td><td> JIMorris </td><td> R0090B500 </td><td> Drivanordning </td></tr><tr><td> Messing Hex Nut, 00-90 </td><td> Materiale </td><td> JIMorris </td><td> N0090B </td><td> Drivanordning </td></tr><tr><td> Messing C260 Strip, ASTM-B36 Tykkelse: 0,025 &quot;, Længde: 12&quot;, Bredde: 1/2 &quot; </td><td> Materiale </td><td> Små dele </td><td> B000FMYU72 </td><td> Drivanordning </td></tr><tr><td> Connector Header, pitch 2mm, male, enkelt række, straigt, 36 stillinger </td><td> Materiale </td><td> Digikey </td><td> 2163S-36-ND </td><td> Drivanordning </td></tr><tr><td> 2-del Sylgard silicium Elastomer </td><td> Materiale </td><td> World Precision Instruments </td><td> SYLG184 </td><td> Til ekstra-isolere sonden </td></tr><tr><td> Decon Contrad 70 Flydende vaskemiddel </td><td> Reagens </td><td> Fisher Scientific </td><td> 04-355 Decon Laboratories Nr.: 1002 </td><td> For at rengøre optagelse steder </td></tr><tr><td> Impedans Conditioning Modul </td><td> Udstyr </td><td> FHC Inc. </td><td> 55-70-0 </td><td> Impedans meter </td></tr><tr><td> niPOD - 32 kanaler </td><td> Udstyr </td><td> Neuronexus </td><td> niPOD -32 </td><td> Impedans meter </td></tr><tr><td> Grip Cement Industrial Grade </td><td> Materiale </td><td> Caulk Dentsply </td><td> 675.571 (pulver) 675.572 (opløsningsmiddel) </td><td> Grip celing </td></tr><tr><td> 1,1 &#39;-dioctadecyl-3, 3,3&#39;, 3&#39;-tetramethylindocarbocyanine perchlorat (&quot;Dil; DiIC18 (3)) </td><td> Reagens </td><td> Invitrogen </td><td> D282 </td><td> At farve proben spor i hjernen </td></tr><tr><td> Rustfrit stål Maskinskrue, Binding Head, Slotted Drive, # 00-90, 1/8 &quot; </td><td> Materiale </td><td> Små dele </td><td> MX-0090-02B </td><td> Ground og reference skruer </td></tr><tr><td> Magnettråd, 20G, nylon-polyurethan, MW80 </td><td> Materiale </td><td> Små dele </td><td> B000IJYRP2 </td><td> Ground og reference wire </td></tr><tr><td> Rustfrit stål Maskinskrue, Binding leder Slotted Drive, # 000-120, 1/16 &quot; </td><td> Materiale </td><td> Små dele </td><td> MX-000.120-01B </td><td> Anchor skruer </td></tr><tr><td> N-3 Alle formål Flux Liquid </td><td> Reagens </td><td> La-Co (MARKAL) </td><td> 23512 </td><td> Gør det muligt at lodde rustfrit stål </td></tr><tr><td> MicroGrid Precision Udvidet Kobber </td><td> Materiale </td><td> Dexmet </td><td> 3 CU6-050 FA </td><td> Kobbernet for on-head Faradays bur </td></tr><tr><td> C &amp; B-METABOND Quick! Cement System - Dentin Activator </td><td> Materiale </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> C &amp; B-METABOND Quick! Cement System - Dental cement </td><td> Materiale </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> Sharp punkt wolfram nål og holder </td><td> Værktøj </td><td> Roboz Kirurgiske instrumenter </td><td> RS-6064 og RS-6061 </td><td> At krogen til at løfte dura </td></tr><tr><td> Carbide Bur HP 1/4 </td><td> Værktøj </td><td> Henry Schein </td><td> 9990013 </td><td></td></tr><tr><td> Paraffin (Granulat) </td><td> Materiale </td><td> Fisher Scientific </td><td> P31-500 </td><td></td></tr><tr><td> Mineral Oil, Light (NF / FCC) </td><td> Materiale </td><td> Fisher Scientific </td><td> O121-1 </td><td></td></tr><tr><td> GC ELECTRONICS 10-114 2-Part epoxylim </td><td> Materiale </td><td> Newark </td><td> 00Z416 </td><td></td></tr><tr><td> Type 1 Litz 21 AWG 40/36 Red Single polyurethan-Nylon (MW80-C) til 0,041 &quot;+ / -0,002&quot; OD </td><td> Materiale </td><td> New England Wire Technologies Corporation </td><td> N28-36E-400 til 2 </td><td> At kablet mellem hovedtrin og forstærkeren </td></tr><tr><td> 32-kanals Very Large Scale Integration hovedtrin, 20x gain </td><td> Udstyr </td><td> Plexon </td><td> HST/32V-G20 </td><td> Hovedtrin </td></tr></tbody></table>

large-scale recording of neurons by movable silicon probes in behaving rodents

En stor udfordring i neurovidenskab linker adfærd til fælles aktivitet af neurale forsamlinger. Forståelse af input-output relationer af neuroner og kredsløb kræver metoder med den rumlige selektivitet og tidsmæssige opløsning, der passer til mekanistisk analyse af neurale ensembler i at opføre sig dyret, dvs optagelse af repræsentativt store prøver af isolerede enkelte neuroner. Ensemble overvågning af neuronal aktivitet har udviklet sig markant i det seneste årti i både små og store brained dyr, herunder forsøgspersoner 1-11. Multiple-site-optagelse med silicium-baserede enheder er særligt effektive på grund af deres skalerbarhed, lille volumen og geometriske udformning. Her beskriver vi metoder til registrering af flere enkelte neuroner og lokale felt potentiale i at opføre gnavere, ved hjælp af kommercielt tilgængelige mikrobearbejdet silicium sonder med skræddersyede tilbehørskomponenter. Der er to grundlæggende muligheder feller er koblet silicium sonder til forforstærkere: printkort og fleksible kabler. Probe forsyningsselskaber ( <a href="http://www.neuronexustech.com/" >http://www.neuronexustech.com/</a> , <a href="http://www.sbmicrosystems.com/">http://www.sbmicrosystems.com/</a> , <a href="http://www.acreo.se/">http://www.acreo.se/</a> ) normalt giver det sammenbindende service og levere prober bundet til printplader eller fleksible ledninger. Her beskriver vi implantation af en 4-skaft, 32-site probe bundet til en fleksibel polyimid kabel, og monteret på en bevægelig mikrodrev. Hvert trin af proben præparatet er mikrodrev konstruktion og operation er illustreret, så at slutbrugeren kan let replikere processen.

Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Vi beskriver metoder til storstilet registrering af flere enkelte enheder og lokale felt potentiale i at opføre gnavere med silicium sonder. Drive fabrikation, probe tilknytning til drevet og sonde implanterings processer er illustreret i tilstrækkelige oplysninger til let replikation.

Storstilet Registrering af neuroner ved Flytbare Silicon Probes at opføre sig Gnavere

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

Research

JoVE Journal

Neuroscience

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of ...

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograd fluorescensmærkning Tillader Målrettet Extracellular Single-unit Optagelse fra Identificerede Neuroner<em&gt; In vivo</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Optagelse Gamma Band Svingninger i Pedunculopontine Nucleus neuroner

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the ...

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Storstilet rekonstruktioner og Independent, Uvildige Clustering Baseret på Morfologiske Metrics at klassificere Neuroner i Selektive populationer

This protocol outlines large-scale reconstructions of neurons combined with the use of independent and unbiased clustering analyses to create a ...

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Kvantificering af endosom og lysosommotiliteter i dyrkede neuroner ved anvendelse af fluorescerende prober

In the brain, membrane trafficking systems play important roles in regulating neuronal functions, such as neuronal morphology, synaptic plasticity, ...

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Implantation af kronisk silicium sonder og optagelse af hippocampus sted celler i en beriget løbebånd apparater

An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced ...

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium billeddannelse af enkelte neuroner i opfører sig Caenorhabditis Elegans

It has become increasingly clear that neural circuit activity in behaving animals differs substantially from that seen in anesthetized or immobilized ...

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

Konstruktion af en forbedret multi triode Hyperdrive for storstilet neurale optagelse i opfører sig rotter

Monitoring the activity patterns of a large population of neurons over many days in awake animals is a valuable technique in the field of systems ...

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Præparater og protokoller for hele cellen Patch klemme optagelsen af Xenopus laevis Tectal neuroner

The Xenopus tadpole retinotectal circuit, comprised of the retinal ganglion cells (RGCs) in the eye which form synapses directly onto neurons in the optic ...