Name: assessing hepatic metabolic changes during progressive colonization of germ-free mouse by 1h nmr spectroscopy
Uploaded: 2011-12-15T13:00:00
Description: Watch this Scientific Journal Video about Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy at JoVE.com

Alle NMR spektra brukes som illustrerende eksempler er hentet fra en tidligere publisert studie 7 som var økonomisk støttet av Nestlé.

1. Kolonisering av bakterie-fritt dyr og prøvetaking <ol><li> Fjern bakterie-fritt dyr fra isolatorer og huse dem i en konvensjonell reindrift rom i bur er utstyrt med filter foran konvensjonelle dyr som vil tjene som kontroller (Figur 1). </li><li> Bland halvparten av kullet (3 dager gammel) tatt fra kontroll konvensjonelle bur med kullet av bakterie-fritt dyr. Hold alltid 1 / 3 av de skitne konvensjonelle kullet hver gang det er nødvendig å fornye den for å opprettholde et nivå av bakterier (holde det minst i 3...

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	<li>Cani, P. D., Delzenne, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microflora+as+a+target+for+energy+and+metabolic.">Gut microflora as a target for energy and metabolic.</a> Curr. Opin. Clin. Nutr. Metab. Care. 10, 729-734 (2007).</li><li>Ley, R. E., Turnbaugh, P. J., Klein, S., Gordon, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbial+ecology:+human+gut+microbes+associated+with+obesity.">Microbial ecology: human gut microbes associated with obesity.</a> Nature. 444, 1022-1023 (2006).</li><li>Raoult, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Obesity+pandemics+and+the+modification+of+digestive+bacterial+flora.">Obesity pandemics and the modification of digestive bacterial flora.</a> Eur. J. Clin. Microbiol. Infect. Dis. 27, 631-634 (2008).</li><li>Turnbaugh, P. J., Backhed, F., Fulton, L., Gordon, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Diet-induced+obesity+is+linked+to+marked+but+reversible+alterations+in+the+mouse+distal+gut+microbiome.">Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome.</a> Cell. Host. Microbe. 3, 213-223 (2008).</li><li>Balcombe, J. P., Barnard, N. D., Sandusky, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Laboratory+routines+cause+animal+stress.">Laboratory routines cause animal stress.</a> Contemp. Top. Lab. Anim. Sci. 43, 42-51 (2004).</li><li>Muyzer, G., Smalla, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+denaturing+gradient+gel+electrophoresis+(DGGE)+and+temperature+gradient+gel+electrophoresis+(TGGE)+in+microbial+ecology.">Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology.</a> Antonie van Leeuwenhoek. 73, 127-141 (1998).</li><li>Claus, S. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colonization-induced+host-gut+microbial+metabolic+interaction.">Colonization-induced host-gut microbial metabolic interaction.</a> MBio. 2, (2011).</li><li>Waters, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+magic+angle+spinning+1H+NMR+spectroscopy+of+intact+liver+and+kidney:+optimization+of+sample+preparation+procedures+and+biochemical+stability+of+tissue+during+spectral+acquisition.">High-resolution magic angle spinning 1H NMR spectroscopy of intact liver and kidney: optimization of sample preparation procedures and biochemical stability of tissue during spectral acquisition.</a> Anal. Biochem. 282, 16-23 (2000).</li><li>Bollard, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+1H+and+1H-13C+magic+angle+spinning+NMR+spectroscopy+of+rat+liver.">High-resolution 1H and 1H-13C magic angle spinning NMR spectroscopy of rat liver.</a> Magnetic resonance in medicine. 44, 201-207 (2000).</li><li>Lindon, J. C., Holmes, E., Nicholson, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pattern+recognition+methods+and+applications+in+biomedical+magnetic+resonance.">Pattern recognition methods and applications in biomedical magnetic resonance.</a> Progress in Nuclear Magnetic Resonance Spectroscopy. 39, 1-40 (2001).</li><li>Tate, A. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Distinction+between+normal+and+renal+cell+carcinoma+kidney+cortical+biopsy+samples+using+pattern+recognition+of+(1)H+magic+angle+spinning+(MAS)+NMR+spectra.">Distinction between normal and renal cell carcinoma kidney cortical biopsy samples using pattern recognition of (1)H magic angle spinning (MAS) NMR spectra.</a> NMR. Biomed. 13, 64-71 (2000).</li><li>Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Topographical+variation+in+metabolic+signatures+of+human+gastrointestinal+biopsies+revealed+by+high-resolution+magic-angle+spinning+1H+NMR+spectroscopy.">Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning 1H NMR spectroscopy.</a> Journal of Proteome Research. 6, 3944-3951 (2007).</li><li>Meiboom, S., Gill, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Modified+spin-echo+method+for+measuring+nuclear+relaxation+times.">Modified spin-echo method for measuring nuclear relaxation times.</a> The review of scientific instruments. 29, 688-691 (1958).</li><li>Nicholson, J. K., Holmes, E., Wilson, I. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+microorganisms,+mammalian+metabolism+and+personalized+health+care.">Gut microorganisms, mammalian metabolism and personalized health care.</a> Nat. Rev. Microbiol. 3, 431-438 (2005).</li><li>Martin, F. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+probiotic+Lactobacillus+paracasei+treatment+on+the+host+gut+tissue+metabolic+profiles+probed+via+magic-angle-spinning+NMR+spectroscopy.">Effects of probiotic Lactobacillus paracasei treatment on the host gut tissue metabolic profiles probed via magic-angle-spinning NMR spectroscopy.</a> Journal of Proteome Research. 6, 1471-1481 (2007).</li><li>Swann, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variation+in+Antibiotic-Induced+Microbial+Recolonization+Impacts+on+the+Host+Metabolic+Phenotypes+of+Rats.">Variation in Antibiotic-Induced Microbial Recolonization Impacts on the Host Metabolic Phenotypes of Rats.</a> J. Proteome. Res. , (2011).</li><li>Jacobs, D. M., Gaudier, E., van Duynhoven, J., Vaughan, E. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Non-digestible+food+ingredients,+colonic+microbiota+and+the+impact+on+gut+health+and+immunity:+a+role+for+metabolomics.">Non-digestible food ingredients, colonic microbiota and the impact on gut health and immunity: a role for metabolomics.</a> Curr. Drug. Metab. 10, 41-54 (2009).</li><li>Beckonert, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-resolution+magic-angle-spinning+NMR+spectroscopy+for+metabolic+profiling+of+intact+tissues.">High-resolution magic-angle-spinning NMR spectroscopy for metabolic profiling of intact tissues.</a> Nat. Protoc. 5, 1019-1032 (2010).</li><li>Hooper, L. V., Sansonetti, P., Zychlinsky, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Methods in microbiology. 31, 559-589 (2002).</li><li>Rahija, R. J., Fox, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ch.+7.">Ch. 7.</a> The mouse in biomedical research. , 217-234 (2007).</li><li>Goodwin, B. L., Ruthven, C. R., Sandler, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gut+flora+and+the+origin+of+some+urinary+aromatic+phenolic+compounds.">Gut flora and the origin of some urinary aromatic phenolic compounds.</a> Biochemical Pharmacology. 47, 2294-2297 (1994).</li><li>Koopman, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term='Normalization'+of+germfree+mice+after+direct+and+indirect+contact+with+mice+having+a+'normal'+intestinal+microflora.">'Normalization' of germfree mice after direct and indirect contact with mice having a 'normal' intestinal microflora.</a> Lab Anim. 20, 286-290 (1986).</li><li>Nishikata, N., Shikata, N., Kimura, Y., Noguchi, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dietary+lipid-dependent+regulation+of+de+novo+lipogenesis+and+lipid+partitioning+by+ketogenic+essential+amino+acids+in+mice.">Dietary lipid-dependent regulation of de novo lipogenesis and lipid partitioning by ketogenic essential amino acids in mice.</a> Nutrition and Diabetes. 1, 1-12 (2011).</li><li>Spagou, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+GC-MS+metabolic+profiling+study+of+plasma+samples+from+mice+on+low-+and+high-fat+diets.">A GC-MS metabolic profiling study of plasma samples from mice on low- and high-fat diets.</a> J. Chromatogr. B. Analyt. Technol. Biomed. Life. Sci. 879, 1467-1475 (2011).</li><li>Sanchez-Patan, F., Monagas, M., Moreno-Arribas, M. V., Bartolome, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+microbial+phenolic+acids+in+human+faeces+by+UPLC-ESI-TQ+MS.">Determination of microbial phenolic acids in human faeces by UPLC-ESI-TQ MS.</a> J. Agric. Food. Chem. 59, 2241-2247 (2011).</li><li>Roux, A., Lison, D., Junot, C., Heilier, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Applications+of+liquid+chromatography+coupled+to+mass+spectrometry-based+metabolomics+in+clinical+chemistry+and+toxicology:+A+review.">Applications of liquid chromatography coupled to mass spectrometry-based metabolomics in clinical chemistry and toxicology: A review.</a> Clin. Biochem. 44, 119-135 (2011).</li><li>Ryan, D., Robards, K., Prenzler, P. D., Kendall, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+and+potential+developments+in+the+analysis+of+urine:+a+review.">Recent and potential developments in the analysis of urine: a review.</a> Anal. Chim. Acta. 684, 8-20 (2011).</li><li>Nagayama, K., Wuthrich, K., Bachmann, P., Ernst, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-dimensional+J-resolved+1H+n.m.r.+spectroscopy+for+studies+of+biological+macromolecules.">Two-dimensional J-resolved 1H n.m.r. spectroscopy for studies of biological macromolecules.</a> Biochem. Biophys. Res. Commun. 78, 99-105 (1977).</li><li>Aue, W. P., Bartholdi, E., Ernst, R. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Two-dimensional+spectroscopy.+Application+to+nuclear+magnetic+resonance.">Two-dimensional spectroscopy. Application to nuclear magnetic resonance.</a> J. Chem. Phys. 64, 2229-2246 (1975).</li><li>Bodenhausen, G., Ruben, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Natural+abundance+15N+NMR+by+enhanced+heteronuclear+spectroscopy.">Natural abundance 15N NMR by enhanced heteronuclear spectroscopy.</a> Chemical. Physics. Letters. 69, 185-189 (1980).</li><li>Fan, T. W. -. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolite+profiling+by+one-+and+two-dimensional+NMR+analysis+of+complex+mixtures.">Metabolite profiling by one- and two-dimensional NMR analysis of complex mixtures.</a> Progress in nuclear magnetic resonance spectroscopy. 28, 161-219 (1996).</li><li>Fan, T., Lane, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Structure-based+profiling+of+metabolites+and+isotopomers+by+NMR.">Structure-based profiling of metabolites and isotopomers by NMR.</a> Progress in Nuclear Magnetic Resonance Spectroscopy. 52, 48-48 (2008).</li><li>Fonville, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+evolution+of+partial+least+squares+models+and+related+chemometric+approaches+in+metabonomics+and+metabolic+phenotyping.">The evolution of partial least squares models and related chemometric approaches in metabonomics and metabolic phenotyping.</a> Journal of Chemometrics. 24, 636-649 (2010).</li><li>Merrifield, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+metabolic+system-wide+characterisation+of+the+pig:+a+model+for+human+physiology.">A metabolic system-wide characterisation of the pig: a model for human physiology.</a> Mol. Biosyst. , (2011).</li><li>Tugnoli, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+characterization+of+human+gastric+mucosa+by+HR-MAS+magnetic+resonance+spectroscopy.">Molecular characterization of human gastric mucosa by HR-MAS magnetic resonance spectroscopy.</a> International Journal of Molecular Medicine. 14, 1065-1071 (2004).</li><li>Sitter, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+HR+MAS+MR+spectroscopic+profiles+of+breast+cancer+tissue+with+clinical+parameters.">Comparison of HR MAS MR spectroscopic profiles of breast cancer tissue with clinical parameters.</a> NMR Biomed. 19, 30-40 (2006).</li><li>Beckonert, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Metabolic+profiling,+metabolomic+and+metabonomic+procedures+for+NMR+spectroscopy+of+urine,+plasma,+serum+and+tissue+extracts.">Metabolic profiling, metabolomic and metabonomic procedures for NMR spectroscopy of urine, plasma, serum and tissue extracts.</a> Nat. Protoc. 2, 2692-2703 (2007).</li></ol>

I denne protokollen, beskrev vi en progressiv kolonisering prosedyre i et åpent miljø for å ytterligere undersøke effekten av tarmen microbiota på levermetabolisme vurderes av en H HR MAS NMR profilering av intakt biopsi. Ulike metoder for kolonisering har blitt beskrevet i litteraturen. De vanligste metodene for å kolonisere dyr med en definert microbiota er oral sonde eller forurenset drikkevann 19,20. Fecal inokulasjon kan også brukes som tidligere beskrevet 21. Koloniseringen ...

Tabell over spesifikke reagenser og utstyr: <table border="1"><tbody><tr><th> Navn på reagens </th><th> Firma </th><th> Katalognummer </th><th> Kommentarer </th></tr><tr><td> 2,5 mm microtube </td><td> New Era </td><td> NE-H5/2.5-V-Br </td><td></td></tr><tr><td> 1,7 mm kapillarrør </td><td> Sigma-Aldrich </td><td> NORS175001 </td><td></td></tr><tr><td> Capillary adapter </td><td> New Era </td><td> NE-325-5/1.7 </td><td></td></tr><tr><td> Utvinning stang </td><td> New Era </td><td> NE-341-5 </td><td></td></tr><tr><td> HR-MAS rotor BL4 med 50 mL sfæriske Teflon spacer kit </td><td> Bruker </td><td> HZ07213 </td><td></td></tr><tr><td> Verktøysett for 50 mL innstikk </td><td> Bruker </td><td> B2950 </td><td></td></tr><tr><td> Advance III 600MHz NMR </td><td> Bruker </td><td></td><td></td></tr><tr><td> 1 H HR MAS NMR solid sonde </td><td> Bruker </td><td></td><td></td></tr><tr><td> Deuterium oksid 99,9% </td><td> Sigma-Aldrich </td><td> 530867-1L </td><td></td></tr><tr><td> 3 - (trimethylsilyl) propionsyre syre-d 4 (TSP) </td><td> Sigma-Aldrich </td><td> 269913 </td><td></td></tr></tbody></table>

assessing hepatic metabolic changes during progressive colonization of germ-free mouse by 1h nmr spectroscopy

Det er velkjent at gut bakterier bidra betydelig til verten homeostase, og gir en rekke fordeler som immune beskyttelse og vitamin syntese. De leverer også verten med en betydelig mengde næringsstoffer, noe som gjør dette økosystemet et vesentlig metabolsk organ. I forbindelse med økende bevis for koblingen mellom tarmflora og metabolsk syndrom, forstå metabolske samspillet mellom verten og dens gut microbiota blir en viktig utfordring i moderne biologi. 1-4 Colonization (også referert til som normalisering prosessen) betegner etablering av mikro-organismer i et tidligere bakterie-fritt dyr. Mens det er en naturlig prosess som skjer ved fødselen, er det også brukt i voksen bakterie-fritt dyr for å kontrollere tarmen floral økosystemet og videre bestemme dens innvirkning på verten metabolismen. En vanlig prosedyre for å kontrollere koloniseringen prosessen er å bruke sonde metoden med en single eller en blanding av mikro-organismer. Denne metoden resulterer i en meget rask kolonisering og presenterer den ulempen ved å være ekstremt stressende 5. Det er derfor nyttig å minimere stress og å få en tregere kolonisering prosess for å observere gradvis virkningen av bakteriell etablering på verten metabolismen. I dette manuskriptet, beskriver vi en prosedyre for å vurdere endring av hepatisk metabolisme under en gradvis kolonisering prosess med et ikke-destruktiv metabolske profilering teknikk. Vi foreslår å overvåke gut mikrobiell kolonisering ved å vurdere gut mikrobiell metabolsk aktivitet reflekteres av urinutskillelse av mikrobiell co-metabolitter av 1 H NMR-baserte metabolske profilering. Dette gir en styrking av stabilitet i tarmen mikrobiell aktivitet utover stabil etablering av tarmen mikrobielle økosystem vanligvis vurdert ved å overvåke fecal bakterier ved DGGE (denaturering gradient gel elektroforese). 6kolonisering foregår i en konvensjonell åpne omgivelser og er initiert av en skitten kull tilgriset av konvensjonelle dyr, som vil fungere som kontroller. Gnagere blir coprophagous dyr, sikrer dette en homogen kolonisering som tidligere beskrevet. 7 Nedsatt metabolske profilering måles direkte fra et intakt leverbiopsi bruker 1 H High Resolution Magisk Angle Spinning NMR-spektroskopi. Dette semi-kvantitative teknikken tilbyr en rask måte å vurdere, uten å skade cellestrukturen, de viktigste metabolittene som triglyserider, glukose og glykogen for å ytterligere estimere komplekse samspillet mellom koloniseringen prosessen og hepatisk metabolisme 7-10. Denne metoden kan også brukes på alle vev biopsi 11,12.

Watch this Scientific Journal Video about Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy at JoVE.com

Assessing Hepatic Metabolic Changes During Progressive Colonization of Germ-free Mouse by 1H NMR Spectroscopy

En progressiv kolonisering prosedyren er beskrevet å vurdere nærmere dens innvirkning på verten hepatisk metabolisme. Colonization er overvåket non invasiv ved å evaluere urinutskillelse av mikrobiell co-metabolitter av NMR-baserte metabolske profilering mens hepatisk metabolisme er vurdert av High Resolution Magisk Angle Spinning (HR MAS) NMR profilering av intakt biopsi.

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En Murine modell av gruppe B<em&gt; Streptococcus</em&gt; Vaginal Colonization

Streptococcus agalactiae (group B Streptococcus, GBS), is a Gram-positive, asymptomatic colonizer of the human gastrointestinal tract and vaginal tract of ...

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molekylær diffusjon i plasmamembraner Primære lymfocytter målt ved fluorescens korrelasjon spektroskopi

Fluorescence correlation spectroscopy (FCS) is a powerful technique for studying the diffusion of molecules within biological membranes with high spatial ...