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Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

DOI :

10.3791/3713-v

7:06 min

December 12th, 2011

December 12th, 2011

38,697 Views

1Genetically Engineered Models and Services, Charles River , 2Research Models and Services, Charles River

Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.

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Mouse Sperm Cryopreservation

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