Name: retrograde perfusion and filling of mouse coronary vasculature as preparation for micro computed tomography imaging
Uploaded: 2012-02-10T13:00:00
Description: Watch this Scientific Journal Video about Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging at JoVE.com

我们感谢凯利·史蒂文斯博士的协议，迈克尔·西蒙斯博士，博士硖Hauch，一般性讨论实验室成员的初步试验。 这项工作是由NIH资助HL087513和P01号HL094374支持。

1。开始前的准备<ol><li>填写与血管舒张缓冲区（4mg / L的罂粟碱+ 1g / L的腺苷在PBS）或4％多聚甲醛（PFA）在PBS压力灌注装置两侧分别。 </li><li>准备填补1:100肝素0.1毫升（5000U/ml股票）和弯曲针〜120度角的斜面向上1 1/2cc胰岛素注射器（带一个永久连接的29G半“针）。做用1毫升注射器装满0.3毫升饱和氯化钾溶液（1 26G半“针）相同。 </li></ol> 2。暴露心脏插管主动脉<ol><li>使用您选?...

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心脏组织有一个非常高的代谢需求，因此需要不断交付的冠状动脉血管的血液供应养分和氧气。疾病的冠状血管，降低因血管狭窄和阻塞的冠状动脉功能，可导致组织缺血缺氧，并把受影响的病人，心肌梗死和心脏肌肉造成不可弥补的损害的风险。一个更好地了解这些船只的疾病状态是必要的，关键我们的学习能力冠状动脉血管的可视化。在这里，我们提出了一个准备前体内成像小鼠冠状?...

<html><table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> 1毫升注射器</td><td> <a href="http://www.bd.com/" target="_blank">碧迪</a> </td><td> BD-309602 </td><td></td></tr><tr><td> 1/2cc 29G半永久连接的针胰岛素注射器</td><td> <a href="http://www.bd.com/" target="_blank">碧迪</a> </td><td> BD-309306 </td><td></td></tr><tr><td> 2“×2”的纱布垫</td><td> Med101store.com </td><td> SKU的2208 </td><td></td></tr><tr><td> 24G¾“Angiocath静脉导管</td><td> <a href="http://www.bd.com/" target="_blank">碧迪</a> </td><td> BD-381112 </td><td></td></tr><tr><td> 26G半“表针</td><td>又长又“碧迪</td><td> BD-305111 </td><td></td></tr><tr><td>腺苷</td><td> <a href="http://www.sigmaaldrich.com/" target="_blank">西格玛</a> </td><td> A9251 </td><td>舒张缓冲PBS 1G /升（与罂粟碱） </td></tr><tr><td>直角格雷夫钳</td><td> <a href="http://www.finescience.com/" target="_blank">罚款科学工具</a> </td><td> 11052-10 </td><td></td></tr><tr><td>棉签涂药：“非无菌</td><td> <a href="http://www.cardinal.com/" target="_blank">卡地纳健康</a> </td><td> C15055-006 </td><td></td></tr><tr><td>弯曲的手术剪刀</td><td> <a href="http://www.finescience.com/" target="_blank">罚款科学工具</a> </td><td> 14085-09 </td><td></td></tr><tr><td>解剖立体光源</td><td> <a href="http://www.nikon.com/" target="_blank">尼康</a> </td><td>不适用</td><td>不适用</td></tr><tr><td>解剖托盘，11.5 x 7.5英寸</td><td> <a href="http://www.coleparmer.com/" target="_blank">科尔-帕默</a> </td><td> YO-10915-12 </td><td>焦油充满牵制鼠标</td></tr><tr><td>精细弯钳</td><td> <a href="http://www.aesculapusa.com/" target="_blank">蛇牌</a> </td><td> FD281R </td><td>需要两个</td></tr><tr><td>肝素5000 U / ml的股票</td><td> <a href="http://www.apppharma.com/" target="_blank">APP的制药有限责任公司</a> </td><td> NDC的63323-047-10 </td><td> 1:100稀释水</td></tr><tr><td>氯化钾</td><td> <a href="http://www.fishersci.com/" target="_blank">费舍尔</a> </td><td> P217 </td><td>在水中的饱和溶液</td></tr><tr><td>氯胺酮（Ketaset），100毫克/毫升股票</td><td>道奇堡，欧弗兰帕克，堪萨斯州，美国</td><td> NDC 0856-2013-01 </td><td>为130毫克/千克体重，混合二甲苯胺噻嗪在0.9％s艾琳</td></tr><tr><td> microfil </td><td> <a href="http://www.flowtech-inc.com/" target="_blank">流技术</a> </td><td> MV-122（黄色）。其他颜色的选择也可以。 </td><td>重量1:1混合，固化剂量的10％。只是前准备，以确保它是很好的混合，注射和涡</td></tr><tr><td>非无菌缝合：6-0，编织丝绸</td><td> <a href="http://www.harvardapparatus.com/" target="_blank">哈佛仪器</a> </td><td> 723287 </td><td></td></tr><tr><td>罂粟碱</td><td> <a href="http://www.americanregent.com/" target="_blank">美国丽晶公司</a> </td><td> NDC 0517-4010-01 </td><td>在舒张缓冲PBS 4毫克/升（与腺苷） </td></tr><tr><td>多聚甲醛</td><td> <a href="http://www.sigmaaldrich.com/" target="_blank">西格玛</a> </td><td> P6148 </td><td>准备为4％的解决方案</td></tr><tr><td>灌流装置</td><td></td><td></td><td>见图2 </td></tr><tr><td>弹簧剪刀</td><td> <a href="http://www.finescience.com/" target="_blank">罚款科学工具</a> </td><td> 15018-10 </td><td></td></tr><tr><td>甲苯噻嗪（Anased），20毫克/ GL股票</td><td> <a href="http://www.lloydlab.com/" target="_blank">劳埃德实验室</a> </td><td> NADA的＃139-236 </td><td>为8.8毫克/公斤体重，氯胺酮，0.9％生理盐水混合</td></tr></tbody></table>

retrograde perfusion and filling of mouse coronary vasculature as preparation for micro computed tomography imaging

了解许多不同的疾病状态的可视化的血管变得越来越重要。虽然一些技术成像血管存在，很少有能够可视化作为一个整体的血管网，同时延伸到了一项决议，包括较小的船只1,2。此外，许多血管铸型技术破坏周围组织，防止进一步分析样品3-5。规避这些问题的方法之一是微型计算机断层扫描（μCT）。 μCT成像扫描分辨率&lt;10微米，是能够产生三维重建血管网，并留下完整的后续分析组织（例如，组织学和形态学）6-11。然而，通过体外 μCT方法成像船只需要，船只可与不透X线的化合物填充。 μCT成像产生血管的准确表示是因为如此，然后船只的可靠和完整的灌装。在这个协议中，我们描述了μCT成像小鼠冠状血管，准备灌装技术。 两个占主导地位的技术填补了冠状动脉血管存在： 在体内通过主动脉插管和逆行灌注（或关闭主动脉弓分支）12-14日 ，或通过Langendorff灌流系统15-17 体外 。在这里，我们描述了在体内主动脉插管的方法已被专门设计，以确保填写所有船只。我们使用低粘度不透X线的化合物称为Microfil填补所有的毛细血管，以及动脉和静脉血管网双方可以通过最小血管灌注。血管灌注使用加压灌注系统的缓冲，然后填写Microfil。为了确保Microfil填补了小的阻力血管，结扎大枝emanating从主动脉，转移到冠状动脉Microfil。一旦填写完成后，挤压Microfil了一些船只，以防止心脏组织的弹性性质，我们结扎灌装后立即访问的主要血管的出口点。因此，我们的技术进行了优化，完整的充填和充填剂的最大保留，使完整的冠状动脉血管网的可视化 - 动脉，毛细血管和静脉的一致好评。

Watch this Scientific Journal Video about Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging at JoVE.com

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

冠状动脉血管的可视化来推进我们的心血管疾病的认识是至关重要的。在这里，我们描述了在微型计算机断层扫描成像（μCT）准备，与不透X线的硅橡胶（Microfil）灌注小鼠冠状动脉血管的方法。

微型计算机断层扫描成像制备小鼠冠状动脉血管逆行灌注和灌装

Visualization of the vasculature is becoming increasingly important for understanding many different disease states. While several techniques exist for ...

Research

JoVE Journal

Medicine

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

利用X射线计算机断层扫描在小鼠肥胖模型的FAT卷的分割和测量

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

科研

医学

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

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Preclinical research in animal models of peripheral arterial disease plays a vital role in testing the efficacy of therapeutic agents designed to ...

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

评估人体脂肪注射生存能力的裸鼠与微型计算机断层扫描

Lipotransfer is a vital tool in the surgeon&#8217;s armamentarium for the treatment of soft tissue deficits of throughout the body. Fat is the ideal soft ...

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer

采用微计算机断层扫描对肿瘤发展的评估和后续反应来治疗肺癌的小鼠模型

Lung cancer is the most lethal cancer in the world. Intensive research is ongoing worldwide to identify new therapies for lung cancer. Several mouse ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

结合近红外荧光成像和微计算机断层扫描直接可视化脑血栓

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans

非门控断层扫描的冠状动脉钙化识别

Coronary artery calcification (CAC) provides an objective measure of coronary artery disease and can readily be identified on non-gated computed ...

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

小型动物正电子发射断层扫描/计算机断层扫描中的连续血液采样支持动脉输入函数的测量

For quantitative analysis and bio-kinetic modeling of positron emission tomography/computed tomography (PET/CT) data, the determination of the temporal ...

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

用于慢性病大型哺乳动物心脏模型对比增强显影显微计算机断层扫描的组织制备技术

Structural remodeling is a common consequence of chronic pathological stresses imposed on the heart. Understanding the architectural and compositional ...

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

DUCT：双树脂铸造，然后进行微型计算机断层扫描，用于3D肝脏分析

The liver is the biggest internal organ in humans and mice, and high auto-fluorescence presents a significant challenge for assessing the ...

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

基于计算机断层扫描的小鼠后肢血管三维可视化的样品制备

Blood vessels are complex networks with tree-like structures, and vascular networks are essential for maintaining both circulation and maintaining organ ...