Name: retrograde perfusion and filling of mouse coronary vasculature as preparation for micro computed tomography imaging
Uploaded: 2012-02-10T13:00:00
Description: Watch this Scientific Journal Video about Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging at JoVE.com

Ringraziamo il Dott. Kelly Stevens per le prove iniziali del protocollo, il dottor Michael Simons, Hauch Kip Dr., e membri di entrambi i loro laboratori per la discussione generale. Questo lavoro è l&#39;assistenza di sovvenzioni NIH HL087513 e P01 HL094374.

1. Preparativi prima di iniziare <ol><li> Riempire ciascun lato dell&#39;apparecchio perfusione pressione con tampone Vasodilator (4 mg / L papaverina + 1 g / L adenosina in PBS) o 4% paraformaldeide (PFA) in PBS, rispettivamente. </li><li> Preparare una siringa 1/2cc insulina (con una 29G permanentemente attaccato ½ &quot;needle), riempiendolo con 0,1 ml di eparina 1:100 (stock 5000U/ml) e piegando l&#39;ago ~ 120 gradi con il smussata. Do the stesso con una siringa da 1 ml (con 26G ½ &quot;spillo) riempita con 0,3...

<ol>
	<li>Couffinhal, T., Dufourcq, P., Barandon, L., Leroux, L., Duplaa, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mouse+models+to+study+angiogenesis+in+the+context+of+cardiovascular+diseases.">Mouse models to study angiogenesis in the context of cardiovascular diseases.</a> Front. Biosci. 14, 3310-3325 (2009).</li><li>Zagorchev, L., Mulligan-Kehoe, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+imaging+of+vessels+in+mouse+models+of+disease.">Molecular imaging of vessels in mouse models of disease.</a> Eur. J. Radiol. 70, 305-311 (2009).</li><li>Krucker, T., Lang, A., Meyer, E. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=New+polyurethane-based+material+for+vascular+corrosion+casting+with+improved+physical+and+imaging+characteristics.">New polyurethane-based material for vascular corrosion casting with improved physical and imaging characteristics.</a> Microsc. Res. Tech. 69, 138-147 (2006).</li><li>Murakami, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood+flow+patterns+in+the+rat+pancreas:+a+simulative+demonstration+by+injection+replication+and+scanning+electron+microscopy.">Blood flow patterns in the rat pancreas: a simulative demonstration by injection replication and scanning electron microscopy.</a> Microsc. Res. Tech. 37, 497-508 (1997).</li><li>Icardo, J. M., Colvee, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Origin+and+course+of+the+coronary+arteries+in+normal+mice+and+in+iv/iv+mice.">Origin and course of the coronary arteries in normal mice and in iv/iv mice.</a> J. Anat. 199, 473-482 (2001).</li><li>Beighley, P. E., Thomas, P. J., Jorgensen, S. M., Ritman, E. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+architecture+of+myocardial+microcirculation+in+intact+rat+heart:+a+study+with+micro-CT.">3D architecture of myocardial microcirculation in intact rat heart: a study with micro-CT.</a> Adv. Exp. Med. Biol. 430, 165-175 (1997).</li><li>Bentley, M. D., Ortiz, M. C., Ritman, E. L., Romero, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+microcomputed+tomography+to+study+microvasculature+in+small+rodents.">The use of microcomputed tomography to study microvasculature in small rodents.</a> Am. J. Physiol. Regul. Integr. Comp. Physiol. 282, R1267-R1279 (2002).</li><li>Jorgensen, S. M., Demirkaya, O., Ritman, E. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+imaging+of+vasculature+and+parenchyma+in+intact+rodent+organs+with+X-ray+micro-CT.">Three-dimensional imaging of vasculature and parenchyma in intact rodent organs with X-ray micro-CT.</a> Am. J. Physiol. 275, H1103-H1114 (1998).</li><li>Marxen, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MicroCT+scanner+performance+and+considerations+for+vascular+specimen+imaging.">MicroCT scanner performance and considerations for vascular specimen imaging.</a> Med. Phys. 31, 305-313 (2004).</li><li>Zagorchev, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro+computed+tomography+for+vascular+exploration.">Micro computed tomography for vascular exploration.</a> J. Angiogenes. Res. 2, 7-7 (2010).</li><li>Heinzer, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hierarchical+microimaging+for+multiscale+analysis+of+large+vascular+networks.">Hierarchical microimaging for multiscale analysis of large vascular networks.</a> Neuroimage. 32, 626-636 (2006).</li><li>Dedkov, E. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synectin/syndecan-4+regulate+coronary+arteriolar+growth+during+development.">Synectin/syndecan-4 regulate coronary arteriolar growth during development.</a> Dev. Dyn. 236, 2004-2010 (2007).</li><li>Gossl, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+anatomy+and+hemodynamic+characteristics+of+vasa+vasorum+in+the+walls+of+porcine+coronary+arteries.">Functional anatomy and hemodynamic characteristics of vasa vasorum in the walls of porcine coronary arteries.</a> Anat. Rec. A. Discov. Mol. Cell. Evol. Biol. 272, 526-537 (2003).</li><li>Rodriguez-Porcel, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Altered+myocardial+microvascular+3D+architecture+in+experimental+hypercholesterolemia.">Altered myocardial microvascular 3D architecture in experimental hypercholesterolemia.</a> Circulation. 102, 2028-2030 (2000).</li><li>Bell, R. M., Mocanu, M. M., Yellon, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Retrograde+heart+perfusion:+The+Langendorff+technique+of+isolated+heart+perfusion.">Retrograde heart perfusion: The Langendorff technique of isolated heart perfusion.</a> J. Mol. Cell. Cardiol. 50, 940-950 (2011).</li><li>Skrzypiec-Spring, M., Grotthus, B., Szelag, A., Schulz, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolated+heart+perfusion+according+to+Langendorff---still+viable+in+the+new+millennium.">Isolated heart perfusion according to Langendorff---still viable in the new millennium.</a> J. Pharmacol. Toxicol. Methods. 55, 113-126 (2007).</li><li>Toyota, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascular+endothelial+growth+factor+is+required+for+coronary+collateral+growth+in+the+rat.">Vascular endothelial growth factor is required for coronary collateral growth in the rat.</a> Circulation. 112, 2108-2113 (2005).</li><li>Lavine, K. J., Long, F., Choi, K., Smith, C., Ornitz, D. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hedgehog+signaling+to+distinct+cell+types+differentially+regulates+coronary+artery+and+vein+development.">Hedgehog signaling to distinct cell types differentially regulates coronary artery and vein development.</a> Development. 135, 3161-3171 (2008).</li><li>Cheema, A. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adventitial+microvessel+formation+after+coronary+stenting+and+the+effects+of+SU11218,+a+tyrosine+kinase+inhibitor.">Adventitial microvessel formation after coronary stenting and the effects of SU11218, a tyrosine kinase inhibitor.</a> J. Am. Coll. Cardiol. 47, 1067-1075 (2006).</li><li>Lametschwandtner, A., Lametschwandtner, U., Weiger, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scanning+electron+microscopy+of+vascular+corrosion+casts--technique+and+applications:+updated+review.">Scanning electron microscopy of vascular corrosion casts--technique and applications: updated review.</a> Scanning Microsc. 4, 889-941 (1990).</li><li>Schneider, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Simultaneous+3D+visualization+and+quantification+of+murine+bone+and+bone+vasculature+using+micro-computed+tomography+and+vascular+replica.">Simultaneous 3D visualization and quantification of murine bone and bone vasculature using micro-computed tomography and vascular replica.</a> Microsc. Res. Tech. 72, 690-701 (2009).</li><li>Manelli, A., Sangiorgi, S., Binaghi, E., Raspanti, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+analysis+of+SEM+images+of+corrosion+casting+using+adaptive+stereo+matching.">3D analysis of SEM images of corrosion casting using adaptive stereo matching.</a> Microscopy Research and Technique. 70, 350-354 (2007).</li><li>Alanentalo, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tomographic+molecular+imaging+and+3D+quantification+within+adult+mouse+organs.">Tomographic molecular imaging and 3D quantification within adult mouse organs.</a> Nat. Meth. 4, 31-33 (2007).</li><li>Quintana, L., Sharpe, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Optical projection tomography of vertebrate embryo development. , 586-594 (2011).</li><li>Walls, J. R., Coultas, L., Rossant, J., Henkelman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-Dimensional+Analysis+of+Vascular+Development+in+the+Mouse+Embryo.">Three-Dimensional Analysis of Vascular Development in the Mouse Embryo.</a> PLoS ONE. 3, e2853-e2853 (2008).</li><li>Chalothorn, D., Clayton, J. A., Zhang, H., Pomp, D., Faber, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Collateral+density,+remodeling,+and+VEGF-A+expression+differ+widely+between+mouse+strains.">Collateral density, remodeling, and VEGF-A expression differ widely between mouse strains.</a> Physiol. Genomics. 30, 179-191 (2007).</li><li>Behm, C. Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+Imaging+of+Endothelial+Vascular+Cell+Adhesion+Molecule-1+Expression+and+Inflammatory+Cell+Recruitment+During+Vasculogenesis+and+Ischemia-Mediated+Arteriogenesis.">Molecular Imaging of Endothelial Vascular Cell Adhesion Molecule-1 Expression and Inflammatory Cell Recruitment During Vasculogenesis and Ischemia-Mediated Arteriogenesis.</a> Circulation. 117, 2902-2911 (2008).</li><li>Carr, C. L., Lindner, J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Myocardial+perfusion+imaging+with+contrast+echocardiography.">Myocardial perfusion imaging with contrast echocardiography.</a> Curr. Cardiol. Rep. 10, 233-239 (2008).</li><li>Leong-Poi, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+Endogenous+and+Therapeutic+Arteriogenesis+by+Contrast+Ultrasound+Molecular+Imaging+of+Integrin+Expression.">Assessment of Endogenous and Therapeutic Arteriogenesis by Contrast Ultrasound Molecular Imaging of Integrin Expression.</a> Circulation. 111, 3248-3254 (2005).</li><li>Villanueva, F. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubbles+Targeted+to+Intercellular+Adhesion+Molecule-1+Bind+to+Activated+Coronary+Artery+Endothelial+Cells.">Microbubbles Targeted to Intercellular Adhesion Molecule-1 Bind to Activated Coronary Artery Endothelial Cells.</a> Circulation. 98, 1-5 (1998).</li><li>Wei, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+of+Myocardial+Blood+Flow+With+Ultrasound-Induced+Destruction+of+Microbubbles+Administered+as+a+Constant+Venous+Infusion.">Quantification of Myocardial Blood Flow With Ultrasound-Induced Destruction of Microbubbles Administered as a Constant Venous Infusion.</a> Circulation. 97, 473-483 (1998).</li><li>Beckmann, N., Stirnimann, R., Bochelen, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High-Resolution+Magnetic+Resonance+Angiography+of+the+Mouse+Brain:+Application+to+Murine+Focal+Cerebral+Ischemia+Models.">High-Resolution Magnetic Resonance Angiography of the Mouse Brain: Application to Murine Focal Cerebral Ischemia Models.</a> Journal of Magnetic Resonance. 140, 442-450 (1999).</li><li>Kobayashi, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+MR+angiography+of+intratumoral+vasculature+using+a+novel+macromolecular+MR+contrast+agent.">3D MR angiography of intratumoral vasculature using a novel macromolecular MR contrast agent.</a> Magnetic Resonance in Medicine. 46, 579-585 (2001).</li><li>Nezafat, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=B1-insensitive+T2+preparation+for+improved+coronary+magnetic+resonance+angiography+at+3+T.">B1-insensitive T2 preparation for improved coronary magnetic resonance angiography at 3 T.</a> Magn. Reson. Med. 55, 858-864 (2006).</li><li>Wagner, S., Helisch, A., Ziegelhoeffer, T., Bachmann, G., Schaper, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+angiography+of+collateral+vessels+in+a+murine+femoral+artery+ligation+model.">Magnetic resonance angiography of collateral vessels in a murine femoral artery ligation model.</a> NMR in Biomedicine. 17, 21-27 (2004).</li><li>Cochet, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+MR+angiography+and+velocity+measurement+in+mice+coronary+arteries+at+9.4+T:+assessment+of+coronary+flow+velocity+reserve.">In vivo MR angiography and velocity measurement in mice coronary arteries at 9.4 T: assessment of coronary flow velocity reserve.</a> Radiology. , 254-441 (2010).</li></ol>

Tessuto cardiaco ha una forte domanda metabolica, e richiede pertanto un costante rifornimento di sostanze nutritive e ossigeno dal sangue erogata dal sistema vascolare coronarico. Malattie dei vasi coronarici, che riducono la funzione a causa di stenosi coronarica nave e blocco, possono portare a ipossia tissutale e di ischemia, e mettere i pazienti affetti a rischio di infarto miocardico e danni irreparabili al muscolo cardiaco. Una migliore comprensione dello stato di malattia di questi vasi è necessario, e critico ...

<html><table border="1"><tbody><tr><td> Nome del reattivo </td><td> Azienda </td><td> Numero di catalogo </td><td> Comments </td></tr><tr><td> 1 ml siringhe </td><td> <a href="http://www.bd.com/" target="_blank">Becton Dickinson</a> </td><td> BD-309602 </td><td></td></tr><tr><td> Siringhe da insulina 1/2cc con aghi fissi ½ 29G &#39; </td><td> <a href="http://www.bd.com/" target="_blank">Becton Dickinson</a> </td><td> BD-309306 </td><td></td></tr><tr><td> Garza 2 &quot;x 2&quot; pastiglie </td><td> Med101store.com </td><td> SKU 2208 </td><td></td></tr><tr><td> 24G ¾ &quot;Angiocath IV catetere </td><td> <a href="http://www.bd.com/" target="_blank">Becton Dickinson</a> </td><td> BD-381112 </td><td></td></tr><tr><td> 26G ½ &quot;aghi calibro </td><td>lank &quot;&gt; Becton Dickinson </td><td> BD-305111 </td><td></td></tr><tr><td> L&#39;adenosina </td><td> <a href="http://www.sigmaaldrich.com/" target="_blank">Sigma</a> </td><td> A9251 </td><td> 1 g / L in PBS per Buffer Vasodilatazione (con papaverina) </td></tr><tr><td> Pinze angolari Graefe </td><td> <a href="http://www.finescience.com/" target="_blank">Strumenti di Scienza Fine</a> </td><td> 11052-10 </td><td></td></tr><tr><td> Cotton-punte: 6 applicatori &quot;non sterile </td><td> <a href="http://www.cardinal.com/" target="_blank">Cardinal Health</a> </td><td> C15055-006 </td><td></td></tr><tr><td> Forbici chirurgiche curve </td><td> <a href="http://www.finescience.com/" target="_blank">Strumenti di Scienza Fine</a> </td><td> 14085-09 </td><td></td></tr><tr><td> Stereoscopio dissezione e la sorgente di luce </td><td> <a href="http://www.nikon.com/" target="_blank">Nikon</a> </td><td> NA </td><td> NA </td></tr><tr><td> Vassoio dissezione, 11,5 x 7,5 pollici </td><td> <a href="http://www.coleparmer.com/" target="_blank">Cole-Parmer</a> </td><td> YO-10.915-12 </td><td> Riempito con tar per appuntare premuto il mouse </td></tr><tr><td> Fine pinze curve </td><td> <a href="http://www.aesculapusa.com/" target="_blank">Aesculap</a> </td><td> FD281R </td><td> Hai bisogno di due </td></tr><tr><td> L&#39;eparina, 5000 U / ml magazzino </td><td> <a href="http://www.apppharma.com/" target="_blank">APP Pharmaceuticals LLC</a> </td><td> NDC 63323-047-10 </td><td> Diluizione 1:100 in acqua </td></tr><tr><td> KCl </td><td> <a href="http://www.fishersci.com/" target="_blank">Pescatore</a> </td><td> P217 </td><td> Soluzione satura di H2O </td></tr><tr><td> Ketamina (Ketaset), 100 mg / ml magazzino </td><td> Fort Dodge, Overland Park, KS, USA </td><td> NDC 0856-2013-01 </td><td> Misto di 130 mg / kg di peso corporeo, con Xylazina allo 0,9% sAline </td></tr><tr><td> Microfil </td><td> <a href="http://www.flowtech-inc.com/" target="_blank">Flusso Tech</a> </td><td> MV-122 (giallo). Altre opzioni di colore sono inoltre disponibili. </td><td> Mescolare 01:01 in peso, con il 10% in volume di catalizzatore. Preparare immediatamente prima dell&#39;iniezione, e vortex per assicurarsi che sia ben amalgamato </td></tr><tr><td> Non sterile di sutura: 6-0, seta intrecciata </td><td> <a href="http://www.harvardapparatus.com/" target="_blank">Harvard Apparatus</a> </td><td> 723287 </td><td></td></tr><tr><td> Papaverina </td><td> <a href="http://www.americanregent.com/" target="_blank">Americano Regent Inc.</a> </td><td> NDC 0517-4010-01 </td><td> 4 mg / L in PBS per Buffer Vasodilatazione (con adenosina) </td></tr><tr><td> Paraformaldeide </td><td> <a href="http://www.sigmaaldrich.com/" target="_blank">Sigma</a> </td><td> P6148 </td><td> Preparato come soluzione al 4% </td></tr><tr><td> Perfusione Apparatus </td><td></td><td></td><td> Vedere la figura 2 </td></tr><tr><td> Primavera Forbici </td><td> <a href="http://www.finescience.com/" target="_blank">Strumenti di Scienza Fine</a> </td><td> 15018-10 </td><td></td></tr><tr><td> Xilazina (Anased), 20 mg / gl magazzino </td><td> <a href="http://www.lloydlab.com/" target="_blank">Lloyd Labs</a> </td><td> NADA # 139-236 </td><td> Misto di 8,8 mg / kg di peso corporeo, con ketamina in soluzione salina 0,9% </td></tr></tbody></table>

retrograde perfusion and filling of mouse coronary vasculature as preparation for micro computed tomography imaging

Visualizzazione della vascolarizzazione sta diventando sempre più importante per capire molti stati patologici diversi. Mentre esistono diverse tecniche per l&#39;imaging vascolare, pochi sono in grado di visualizzare la rete vascolare nel suo insieme, mentre si estende a una risoluzione che include il 1,2 imbarcazioni più piccole. Inoltre, molte tecniche di fusione vascolari distruggere il tessuto circostante, impedendo ulteriori analisi del campione 3-5. Un metodo che elude questi temi è micro-Tomografia Computerizzata (μCT). μCT possibile eseguire la scansione di immagini con risoluzione inferiore a 10 micron, è capace di produrre ricostruzioni 3D della rete vascolare, e lascia intatto il tessuto per l&#39;analisi successiva (ad esempio, l&#39;istologia e la morfometria) 6-11. Tuttavia, navi imaging di ex vivo metodi μCT richiede che i vasi da riempire con un composto radiopaco. Come tale, la rappresentazione accurata di vasi prodotta da μCT immagini è subordinatoriempimento affidabile e completa dei vasi. In questo protocollo, si descrive una tecnica per il riempimento di vasi coronarici del mouse in preparazione per l&#39;imaging μCT. Due tecniche predominano esistono per il riempimento del sistema vascolare coronarico: in vivo via cannulazione e perfusione retrograda dell&#39;aorta (o di un ramo dell&#39;arco aortico) 12-14, o ex vivo mediante un sistema di perfusione Langendorff 15-17. Qui si descrive un metodo in cannulazione aortica vivo che è stato specificamente progettato per garantire il riempimento di tutte le navi. Usiamo un composto a bassa viscosità radiopaco chiamato Microfil che può perfondere attraverso i piccoli vasi per riempire tutti i capillari, nonché entrambi i lati arterioso e venoso della rete vascolare. Le navi sono perfusi con tampone utilizzando un sistema di pressione di perfusione, e poi riempito con Microfil. Per garantire che Microfil riempie i piccoli vasi di resistenza superiori, abbiamo legare i rami di grandi dimensioni emanating dall&#39;aorta, che devia il Microfil nelle coronarie. Una volta che il riempimento è completata, per impedire la natura elastica del tessuto cardiaco da spremere fuori Microfil di alcuni vasi, si legare accessibili i punti importanti uscita vascolari immediatamente dopo il riempimento. Pertanto, la nostra tecnica è ottimizzato per il riempimento completo e la ritenzione massimo dell&#39;agente di riempimento, permettendo la visualizzazione della completa rete vascolare coronarico -, capillari, arterie e vene simili.

Watch this Scientific Journal Video about Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging at JoVE.com

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Visualizzazione dei vasi coronarici è fondamentale per avanzare la nostra comprensione delle malattie cardiovascolari. Qui si descrive un metodo per la perfusione vascolare coronarica murino con una gomma di silicone radiopaco (Microfil), in preparazione per le micro-Tomografia Computerizzata (μCT) imaging.

Perfusione retrograda e riempimento del sistema vascolare coronarico mouse come preparazione per la Micro Computed Tomography Imaging

Visualization of the vasculature is becoming increasingly important for understanding many different disease states. While several techniques exist for ...

Research

JoVE Journal

Medicine

Segmentation and Measurement of Fat Volumes in Murine Obesity Models Using X-ray Computed Tomography

Segmentazione e misurazione dei volumi FAT nei modelli murini di obesità Utilizzo di X-ray Tomografia Computerizzata

Obesity is associated with increased morbidity and mortality as well as reduced metrics in quality of life.1 Both environmental and genetic factors are ...

Ricerca

Medicina

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Osmotica Drug Delivery per arti posteriori ischemico e la perfusione dei vasi con Microfil per Micro-Tomografia Computerizzata Imaging

Preclinical research in animal models of peripheral arterial disease plays a vital role in testing the efficacy of therapeutic agents designed to ...

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Valutazione della solidità del grasso umano iniezione in topi nudi con Micro-Tomografia Computerizzata

Lipotransfer is a vital tool in the surgeon&#8217;s armamentarium for the treatment of soft tissue deficits of throughout the body. Fat is the ideal soft ...

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer

Utilizzando la tomografia micro-computerizzata per la valutazione di sviluppo del tumore e follow-up di risposta al trattamento in un modello murino di cancro ai polmoni

Lung cancer is the most lethal cancer in the world. Intensive research is ongoing worldwide to identify new therapies for lung cancer. Several mouse ...

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Imaging fluorescente combinata vicino infrarosso e la tomografia micro-computerizzata per Direttamente Visualizzazione cerebrale tromboembolie

Direct thrombus imaging visualizes the root cause of thromboembolic infarction. Being able to image thrombus directly allows far better investigation of ...

Identifying Coronary Artery Calcification on Non-gated Computed Tomography Scans

Identificazione di calcificazione dell'arteria coronaria su Non-gated scansioni con tomografia computerizzata

Coronary artery calcification (CAC) provides an objective measure of coronary artery disease and can readily be identified on non-gated computed ...

Continuous Blood Sampling in Small Animal Positron Emission Tomography/Computed Tomography Enables the Measurement of the Arterial Input Function

Il campionamento continuo del sangue nella tomografia a emissione di positroni di piccole dimensioni/tomografia computerizzata consente la misurazione della funzione di input arteriosa

For quantitative analysis and bio-kinetic modeling of positron emission tomography/computed tomography (PET/CT) data, the determination of the temporal ...

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Tecniche di preparazione dei tessuti per l'imaging micromografico computerizzato con contrasto di modelli cardiaci di mammiferi di grandi dimensioni con malattia cronica

Structural remodeling is a common consequence of chronic pathological stresses imposed on the heart. Understanding the architectural and compositional ...

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

DUCT: Double Resin Casting seguito da Tomografia Micro-Computerizzata per Analisi 3D del Fegato

The liver is the biggest internal organ in humans and mice, and high auto-fluorescence presents a significant challenge for assessing the ...

Sample Preparation for Computed Tomography-based Three-dimensional Visualization of Murine Hind-limb Vessels

Preparazione del campione per la visualizzazione tridimensionale basata sulla tomografia computerizzata dei vasi dell'arto posteriore murino

Blood vessels are complex networks with tree-like structures, and vascular networks are essential for maintaining both circulation and maintaining organ ...