Name: murine model of allergen induced asthma
Uploaded: 2012-05-14T12:00:00
Description: Watch this Scientific Journal Video about Murine Model of Allergen Induced Asthma at JoVE.com

Questo lavoro è stato supportato dal NIH di Grant HL093196 (RCR) e la ricerca di Atlanta e Education Foundation (AREF).

I. sensibilizzazione agli allergeni e Challenge (vedi Figura 1) A. Per la sfida intratracheale <ol><li> Per la sensibilizzazione iniziale, iniettare maschio o femmina C57BL / 6 o topi BALB / c (6-8 settimane) via intraperitoneale al giorno 0 e anche il giorno 7 con 20 mcg di ovoalbumina (OVA, Sigma-Aldrich, St. Louis, MO) emulsionato in 0,2 ml di soluzione salina tamponata fosfato sterile (PBS) contenente 2 mg di idrossido di alluminio (Sigma-Aldrich) oppure idrossido di allu...

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I modelli animali di malattie allergiche delle vie aeree forniscono importanti strumenti per gli studi relativi ad asma clinico. Un certo numero di modelli differenti, impiegando specie variabili e antigeni, sono stati sviluppati. Il mouse, una specie di laboratorio attraenti e di uso frequente, offre anche una serie di vantaggi per i modelli di malattie allergiche delle vie aeree. 9,10 Sebbene tali modelli non imitare l&#39;asma sotto ogni aspetto, 11 con gli aspetti della malattia cronica che son...

<table border="1"><tbody><tr><td> Nome del materiale </td><td> Azienda </td><td> Numero di catalogo </td><td> Comments </td></tr><tr><td> Ovalbumina </td><td> Sigma-Aldrich St. Louis, MO </td><td> A5503 </td><td></td></tr><tr><td> Idrossido di alluminio </td><td> Sigma-Aldrich </td><td> 239186 </td><td></td></tr><tr><td> Acetil-β-methylcholine cloruro </td><td> Sigma-Aldrich </td><td> A2251 </td><td></td></tr><tr><td> Sale di sodio pentobarbital </td><td> Sigma-Aldrich </td><td> P3761 </td><td></td></tr><tr><td> Pletismografia corpo intero (WBP) del sistema </td><td> Buxco Research Systems Wilmington, NC </td><td></td><td> <a href="http://www.buxco.com" target="_blank">http://www.buxco.com</a> </td></tr><tr><td> FlexiVent </td><td>SCIREQ, Inc. Montreal, Canada </td><td></td><td> <a href="http://www.scireq.com" target="_blank">http://www.scireq.com</a> </td></tr><tr><td> Microscopio ottico </td><td> Leica Microsystems, Inc. Buffalo Grove, IL </td><td></td><td></td></tr><tr><td> Cytospin 4 </td><td> Thermo Scientific Asheville, Carolina del Nord </td><td></td><td></td></tr><tr><td> Diff-Quick macchia </td><td> Siemens Newark, DE </td><td> B4132-1A </td><td></td></tr><tr><td> Pipetta ripetitiva </td><td> Tridak Torrington, CT </td><td> STP4001-0025 </td><td></td></tr></tbody></table>

murine model of allergen induced asthma

L&#39;asma è una delle principali cause di morbilità e mortalità, che colpisce circa 300 milioni di persone in tutto il mondo. 1 oltre l&#39;8% della popolazione degli Stati Uniti ha l&#39;asma, con la prevalenza in aumento. 2 Come per altre malattie, modelli animali di malattie allergiche delle vie aeree facilitare notevolmente comprensione della fisiopatologia sottostante, aiutare ad identificare potenziali bersagli terapeutici, e permettere sperimentazioni precliniche di possibili nuove terapie. I modelli di malattia delle vie aeree allergica sono stati sviluppati in diverse specie animali, ma i modelli murini sono particolarmente attraente a causa del basso costo, pronta disponibilità, e ben caratterizzati sistema immunitario di questi animali. 3 disponibilità di una varietà di ceppi transgenici aumenta ulteriormente l&#39;attrattiva di questi modelli. 4 Qui si descrivono due modelli murini di malattie delle vie aeree allergica, sia ovoalbumina utilizzando come antigene. A seguito di sensibilizzazione iniziale mediante iniezione intraperitoneale, un modello Delivtori la sfida antigene per nebulizzazione, l&#39;altro con consegna intratracheale. Questi due modelli offrono vantaggi complementari, con ogni imitando le caratteristiche principali di asma umana. 5 Le caratteristiche principali di asma acuto sono una risposta esagerata delle vie aeree a stimoli come la metacolina (iperresponsività delle vie aeree; AHR) e ricco di infiammazione eosinofila delle vie aeree. Questi sono anche gli effetti di spicco del challenge allergenico nei nostri modelli murini, 5,6 e descrivere le tecniche per la misurazione di loro e quindi valutare gli effetti della manipolazione sperimentale. In particolare, si descrive sia invasive 7 e 8 non invasivo tecniche per la misurazione iperreattività delle vie aeree come pure metodi per valutare l&#39;infiltrazione di cellule infiammatorie nelle vie aeree e del polmone. Cellule infiammatorie delle vie aeree vengono raccolti da lavaggio broncoalveolare istopatologia polmonare mentre viene utilizzato per valutare i marcatori di infiammazione in tutto l&#39;organo. Questetecniche forniscono potenti strumenti per studiare l&#39;asma in modi che non sarebbero possibili in esseri umani.

Watch this Scientific Journal Video about Murine Model of Allergen Induced Asthma at JoVE.com

Murine Model of Allergen Induced Asthma

Modelli murini sperimentali di asma allergico offrono nuove possibilità per studiare la patogenesi della malattia e lo sviluppo di nuove terapie. Questi modelli sono adatti a fattori di misura che regolano la risposta immunitaria allergica, infiammazione delle vie aeree, e fisiopatologia polmonare.

Modello murino di asma indotta da allergene

Asthma is a major cause of morbidity and mortality, affecting some 300 million people throughout the world.1 More than 8% of the US population has asthma, ...

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Murine colitis models are tools that are extensively employed in studies focused on understanding the pathobiology of inflammatory intestinal disorders. ...