Name: preparation of parasagittal slices for the investigation of dorsal-ventral organization of the rodent medial entorhinal cortex
Uploaded: 2012-03-28T13:00:00
Description: Watch this Scientific Journal Video about Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex at JoVE.com

我们感谢他们的支持以下内容：英国联邦奖学金委员会资助（惠普），EPSRC的（惠普），BBSRC的（最惠国待遇）和欧盟的玛丽·居里行动（最惠国）。

1。矢状切片制备 1.1解剖大脑半球所有的动物实验应遵循当地的伦理审查和国家法规。在这里描述实验的情况下，这项工作符合英国的动物（科学规程）行动1986年。我们经常使用颈椎脱位，没有麻醉安乐死之前消除大脑的老鼠。或者鼠标可以末期麻醉，但在这种情况下，它可能是必要的，以确定麻醉的选择，影响神经的属性。 从鼠标的?...

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为了方便调查MEC的电路特性，遵循背腹的组织，我们已经描述了在这里详细为生产矢状切片准备保留MEC的背腹的程度的过程。 关键步骤 卸下从动物大脑 。特别小心，以避免对大脑施加压力。这是比迅速清除大脑更重要。 切片。切片应牢固地粘在地板控股室。在vibratome应该有Z轴振动&lt;2微米，并应与高振幅和?...

<table border="1"><tbody><tr><td></td><td> 切割学联（毫米） </td><td> 标准学联（MM） </td><td> 内部解决方案（MM） </td><td> CASNumber </td><td> 供应商目录编号 </td></tr><tr><td>氯化钠</td><td> 86 </td><td> 124 </td><td></td><td> 7647-14-5 </td><td>西格玛S9888 </td></tr><tr><td>的NaH 2 PO 4计 </td><td> 1.2 </td><td> 1.2 </td><td></td><td> 13472-35-0 </td><td>西格玛71505 </td></tr><tr><td>氯化钾</td><td> 2.5 </td><td> 2.5 </td><td> 10 </td><td> 7447-40-7 </td><td>西格玛P3911 </td></tr><tr><td> 碳酸氢钠 </td><td> 25 </td><td> 25 </td><td></td><td> 144-55-8 </td><td>菲舍尔S/4240 </td></tr><tr><td>葡萄糖</td><td> 25 </td><td> 20 </td><td></td><td> 50-99-7 </td><td>西格玛G5767 </td></tr><tr><td>蔗糖</td><td> 75 </td><td></td><td></td><td> 57-50-1 </td><td>西格玛S5016 </td></tr><tr><td>钙CL 2 </td><td> 0.5 </td><td> 2 </td><td></td><td> 10043-52-4 </td><td>厂商VWR 190464K </td></tr><tr><td> MgCl 2的 </td><td> 7 </td><td> 1 </td><td> 2 </td><td> 7786-30-3 </td><td>西格玛63020 </td></tr><tr><td> ķ葡萄糖</td><td></td><td></td><td> 130 </td><td> 299-27-4 </td><td>西格玛G4500 </td></tr><tr><td>肝素钠</td><td></td><td></td><td> 10 </td><td> 7365-45-9 </td><td>西格玛H3375 </td></tr><tr><td> EGTA </td><td></td><td></td><td> 0.1 </td><td> 67-42-5 </td><td>西格玛E4378 </td></tr><tr><td>那2个 ATP </td><td></td><td></td><td> 2 </td><td> 34369-07-8 </td><td>西格玛A7699 </td></tr><tr><td>钠2的GTP </td><td></td><td></td><td> 0.3 </td><td> 36051-31-7 </td><td>西格玛G8877 </td></tr><tr><td> NaPhospho，肌酸</td><td></td><td></td><td> 10 </td><td> 19333-65-4 </td><td>西格玛P7936 </td></tr><tr><td>生物胞素（可选） </td><td></td><td></td><td> 2.7 </td><td> 576-19-2 </td><td&gt;西格玛B4261 </td></tr></tbody></table> 表1。切割学联，学联标准的K-葡萄糖的内部解决方案食谱。

preparation of parasagittal slices for the investigation of dorsal-ventral organization of the rodent medial entorhinal cortex

在大脑的计算依赖于适当的回应突触输入的神经元。神经元的不同，在他们的补充和细胞膜离子通道的分布，确定他们如何应对突触输入。然而，这些细胞的特性和动物行为的神经元功能之间的关系还不是很清楚。这个问题的办法之一，是研究地形组织的神经回路在单个神经元映射到它们编码的信息或计算的位置，他们携带1。使用这种方法的实验表明调节突触反应，感觉和认知电路2,3基本信息编码的原则。 沿背腹轴内侧嗅皮层（MEC）的空间表示地形的组织提供了一个机会，以建立细胞的机制和计算之间的关系，我空间认知mportant。啮齿类动物的MEC的第二层中的神经元编码的位置，使用网格状发射领域4-6。为MEC的背仓发现的神经元，个别的射击领域，形成了一个网之间的距离是30厘米的顺序，而这个距离在逐步位置更腹侧神经元增加至大于1米。一些研究表明MEC的，像网格射击领域之间的间距，也根据自己的背腹位置不同的第二层的神经元细胞的特性，这表明这些细胞的特性是重要的空间计算2,7-10。 在这里，我们描述程序编制和维持MEC的有利地形组织MEC的神经元的生物物理和解剖特性的调查背腹程度的脑切片的电生理记录。背腹的位置确定列印eurons相对解剖标志是难以建立与使用水平的MEC 7,8,11,12片的协议，准确，因为它是难以确定确切的背腹片的位置参考点。我们所描述的程序，使沿背腹轴的迈克，以及可视化的2,10分子梯度准确和一致的测量记录细胞的位置。成年小鼠（28天）使用的程序已经开发，并已成功受聘小鼠1.5岁。的调整，他们可以用年轻的老鼠或其他啮齿类动物。标准化体系的编制及测量系统的调查将有助于这方面的细胞和微电路的特性。

Watch this Scientific Journal Video about Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex at JoVE.com

Preparation of Parasagittal Slices for the Investigation of Dorsal-ventral Organization of the Rodent Medial Entorhinal Cortex

我们描述了大脑切片，保持背腹轴的内侧嗅皮层（MEC）的编制和电生理记录的程序。由于定位的神经编码如下内MEC的背腹侧组织，这些程序协助调查重要的导航和记忆的细胞机制。

背腹鼠​​类内侧嗅皮层组织的调查矢状窦片的制备

Computation in the brain relies on neurons responding appropriately to their synaptic inputs. Neurons differ in their complement and distribution of ...

Research

JoVE Journal

Neuroscience

Brain Imaging Investigation of the Memory-Enhancing Effect of Emotion

脑成像的情感记忆的促进作用调查

Emotional events tend to be better remembered than non-emotional events1,2. One goal of cognitive and affective neuroscientists is 
to understand the ...

科研

神经科学

Preparation of Acute Subventricular Zone Slices for Calcium Imaging

钙成像的急性下区片的制备

The subventricular zone (SVZ) is one of the two neurogenic zones in the postnatal brain. The SVZ contains densely packed cells, including neural ...

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

同时脑电图，实时测量的啮齿动物的大脑皮质中的神经元活动的乳酸浓度与Optogenetic的操作

Although the brain represents less than 5% of the body  by mass, it utilizes approximately one quarter of the glucose used by the body  at rest1. The ...

A Comprehensive Protocol for Manual Segmentation of the Medial Temporal Lobe Structures

一个全面协议的内侧颞叶结构的手动分割

The present paper describes a comprehensive protocol for manual tracing of the set of brain regions comprising the medial temporal lobe (MTL): amygdala, ...

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

用优化的n-甲基 d-葡乙胺保护恢复法制备急性脑切片

This protocol is a practical guide to the N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. Numerous recent studies have ...

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斜脊髓切片用于神经前根刺激的制备

Electrophysiological recordings from spinal cord slices have proven to be a valuable technique to investigate a wide range of questions, from cellular to ...

Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation

经颅磁刺激在线联合方法对经颅交流电刺激对原发性运动皮层的影响

Transcranial Alternating Current Stimulation (tACS) is a neuromodulatory technique able to act through sinusoidal electrical waveforms in a specific ...

Combined Transcranial Magnetic Stimulation and Electroencephalography of the Dorsolateral Prefrontal Cortex

侧前额叶皮质联合经颅磁刺激与脑电图的研究

Transcranial magnetic stimulation (TMS) is a non-invasive method that produces neural excitation in the cortex by means of brief, time-varying magnetic ...

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice

头脊髓和薄片体外制备心律失常活性

Mammalian inspiratory rhythm is generated from a neuronal network in a region of the medulla called the preB&#246;tzinger complex (pBC), which produces a ...

Stochastic Noise Application for the Assessment of Medial Vestibular Nucleus Neuron Sensitivity In Vitro

随机噪声在Vitro内评估中庭前核神经元敏感性的随机噪声应用

Galvanic vestibular stimulation (GVS) has been shown to improve balance measures in individuals with balance or vestibular impairments. This is proposed ...

背腹鼠​​类内侧嗅皮层组织的调查矢状窦片的制备

背腹鼠类内侧嗅皮层组织的调查矢状窦片的制备