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12.9K Views
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14:56 min
February 8th, 2012
DOI :
10.3791/3807-v
Chapters
0:05
Title
1:39
Titration of Mutagen
5:59
Enrichment of Egress Mutants
8:48
Validation of Mutant Phenotypes by Egress Assays
12:41
Representative Data
10:21
Cloning of Egress Mutants by Limiting Dilution
14:14
Conclusion
Summary
フォワード遺伝学はどのように分子レベルで解明するための強力な方法ですトキソプラズマその宿主細胞から出力する。プロトコルは化学的に寄生虫を突然変異を起こさせるために提供され、誘導される出口の欠陥を持つ変異体を濃縮し、クローニングした変異体の表現型を検証します。
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