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89.6K Views
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09:38 min
June 21st, 2012
DOI :
10.3791/3854-v
Chapters
0:05
Title
1:55
Isolation of Mouse Embryonic Fibroblasts
4:05
Inactivation and Plating MEFs (Feeder Cells Preparation)
5:14
Conditioned Medium Preparation and Measurement of Activin A using ELISA
7:41
Representative Conditioned Medium Preparation Results
9:08
Conclusion
Summary
小鼠胚胎成纤维细胞(MEFs)的质量取决于鼠标CF-1如正确的应变。全能性支持MEFs和条件培养基(厘米),从这些应该包含激活素A,小鬼和TGFβ1的最佳浓度为激活素/ Nodal和FGF通路所需的合作保持自我更新和全能性。
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