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89.6K Views
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09:38 min
June 21st, 2012
DOI :
10.3791/3854-v
Chapters
0:05
Title
1:55
Isolation of Mouse Embryonic Fibroblasts
4:05
Inactivation and Plating MEFs (Feeder Cells Preparation)
5:14
Conditioned Medium Preparation and Measurement of Activin A using ELISA
7:41
Representative Conditioned Medium Preparation Results
9:08
Conclusion
Summary
マウス胚性線維芽細胞(MEF)の品質は、例えば、CF-1のようなマウスの右歪みによって決定されます。これらから得られた多能性·支持のMEFと馴化培地(CM)が共同で動作可能な自己再生および多能性を維持するために、アクチビン/ノードおよびFGF経路に必要なアクチビンA、グレムリンとTGFβ1の最適な濃度を含める必要があります。
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