Name: culturing and applications of rotating wall vessel bioreactor derived 3d epithelial cell models
Uploaded: 2012-04-03T13:00:00
Description: Watch this Scientific Journal Video about Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models at JoVE.com

فإن الكتاب أود أن أشكر بروك هيلم لخبرتها التقنية وأندرو لارسن لتحليل البروتين له. وقد تم تمويل هذا العمل في جزء من مؤسسة أبحاث التنمية البدائل (MMHK) غرانت والمعاهد الوطنية للصحة المعدية الأمراض المنقولة جنسيا ومضادات الجراثيم الموضعية التعاونية مركز البحوث IU19 AI062150-01 (MMHK). نحن الامتنان بيولوجيا التكاثر لإعادة استخدامها من الشخصيات.

يجب أن يتم تنفيذ كل الخطوات تحت BSL-2 الأوضاع في تدفق رقائقي غطاء محرك السيارة. 1. إعداد تفاعلات الاحيائية STLV <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تجميع مفاعل حيوي STLV وفقا لبروتوكول الشركة المصنعة وتنفيذ بروتوكول لضمان ...

<ol>
	<li>Herbst-Kralovetz, M. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+and+comparison+of+toll-like+receptor+expression+and+responsiveness+in+primary+and+immortalized+human+female+lower+genital+tract+epithelia.">Quantification and comparison of toll-like receptor expression and responsiveness in primary and immortalized human female lower genital tract epithelia.</a> Am. J. Reprod. Immunol. 59 (3), 212-224 (2008).</li><li>Hjelm, B. E., Berta, A. N., Nickerson, C. A., Arntzen, C. J., Herbst-Kralovetz, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+characterization+of+a+three-dimensional+organotypic+human+vaginal+epithelial+cell+model.">Development and characterization of a three-dimensional organotypic human vaginal epithelial cell model.</a> Biol. Reprod. 82, 617-627 (2009).</li><li>Khaoustov, V. I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+three-dimensional+assembly+of+human+liver+cells+by+simulated+microgravity.">Induction of three-dimensional assembly of human liver cells by simulated microgravity.</a> In Vitro Cell Dev. Biol. Anim. 35, 501-509 (1999).</li><li>Papadaki, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineering+of+functional+cardiac+muscle:+molecular,+structural,+and+electrophysiological+studies.">Tissue engineering of functional cardiac muscle: molecular, structural, and electrophysiological studies.</a> Am. J. Physiol. Heart Circ. Physiol. 280, 168-178 (2001).</li><li>Ishikawa, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reconstitution+of+hepatic+tissue+architectures+from+fetal+liver+cells+obtained+from+a+three-dimensional+culture+with+a+rotating+wall+vessel+bioreactor.">Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor.</a> J. Biosci. Bioeng. 111, 711-718 (2011).</li><li>Carvalho, H. M., Teel, L. D., Goping, G., O'Brien, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+three-dimensional+tissue+culture+model+for+the+study+of+attach+and+efface+lesion+formation+by+enteropathogenic+and+enterohaemorrhagic+Escherichia+coli.">A three-dimensional tissue culture model for the study of attach and efface lesion formation by enteropathogenic and enterohaemorrhagic Escherichia coli.</a> Cell Microbiol. 7, 1771-1781 (2005).</li><li>Nickerson, C. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+tissue+assemblies:+novel+models+for+the+study+of+Salmonella+enterica+serovar+Typhimurium+pathogenesis.">Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis.</a> Infection and Immunity. 69, 7106-7120 (2001).</li><li>Honer zu Bentrup, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+organotypic+models+of+human+colonic+epithelium+to+study+the+early+stages+of+enteric+salmonellosis.">Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis.</a> Microbes Infect. 8, 1813-1825 (2006).</li><li>Carterson, A. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A549+lung+epithelial+cells+grown+as+three-dimensional+aggregates:+alternative+tissue+culture+model+for+Pseudomonas+aeruginosa+pathogenesis.">A549 lung epithelial cells grown as three-dimensional aggregates: alternative tissue culture model for Pseudomonas aeruginosa pathogenesis.</a> Infection and Immunity. 73, 1129-1140 (2005).</li><li>Smith, Y. C., Grande, K. K., Rasmussen, S. B., O'Brien, A. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Novel+three-dimensional+organoid+model+for+evaluation+of+the+interaction+of+uropathogenic+Escherichia+coli+with+terminally+differentiated+human+urothelial+cells.">Novel three-dimensional organoid model for evaluation of the interaction of uropathogenic Escherichia coli with terminally differentiated human urothelial cells.</a> Infection and Immunity. 74, 750-757 (2006).</li><li>Sainz, B., TenCate, V., Uprichard, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+Huh7+cell+culture+system+for+the+study+of+Hepatitis+C+virus+infection.">Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection.</a> Virol J. 6, 103 (2009).</li><li>Duray, P. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Invasion+of+human+tissue+ex+vivo+by+Borrelia+burgdorferi.">Invasion of human tissue ex vivo by Borrelia burgdorferi.</a> J. Infect Dis. 191, 1747-1754 (2005).</li><li>Margolis, L. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lymphocyte+trafficking+and+HIV+infection+of+human+lymphoid+tissue+in+a+rotating+wall+vessel+bioreactor.">Lymphocyte trafficking and HIV infection of human lymphoid tissue in a rotating wall vessel bioreactor.</a> AIDS Res. Hum. Retroviruses. 13, 1411-1420 (1997).</li><li>Reed, L. J., Muench, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+simple+method+of+estimating+fifty+percent+endpoints.">A simple method of estimating fifty percent endpoints.</a> Am. J. Hyg. 27, 493-497 (1938).</li><li>Beer, B. E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+preclinical+testing+of+nonoxynol-9+as+potential+anti-human+immunodeficiency+virus+microbicide:+a+retrospective+analysis+of+results+from+five+laboratories.">In vitro preclinical testing of nonoxynol-9 as potential anti-human immunodeficiency virus microbicide: a retrospective analysis of results from five laboratories.</a> Antimicrob Agents Chemother. 50, 713-723 (2006).</li><li>Hickey, D. K., Patel, M. V., Fahey, J. V., Wira, C. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Innate+and+adaptive+immunity+at+mucosal+surfaces+of+the+female+reproductive+tract:+stratification+and+integration+of+immune+protection+against+the+transmission+of+sexually+transmitted+infections.">Innate and adaptive immunity at mucosal surfaces of the female reproductive tract: stratification and integration of immune protection against the transmission of sexually transmitted infections.</a> J. Reprod. Immunol. 88, 185-194 (2011).</li><li>Andersch-Bjorkman, Y., Thomsson, K. A., Holmen Larsson, J. M., Ekerhovd, E., Hansson, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large+scale+identification+of+proteins,+mucins,+and+their+O-glycosylation+in+the+endocervical+mucus+during+the+menstrual+cycle.">Large scale identification of proteins, mucins, and their O-glycosylation in the endocervical mucus during the menstrual cycle.</a> Mol. Cell Proteomics. 6, 708-716 (2007).</li><li>Barrila, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+cell+culture+models:+Innovative+platforms+for+studying+host-pathogen+interactions.">3D cell culture models: Innovative platforms for studying host-pathogen interactions.</a> Nature Reviews Microbiology. 8, 791-801 (2010).</li><li>Vamvakidou, A. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Heterogeneous+breast+tumoroids:+An+in+vitro+assay+for+investigating+cellular+heterogeneity+and+drug+delivery.">Heterogeneous breast tumoroids: An in vitro assay for investigating cellular heterogeneity and drug delivery.</a> J. Biomol Screen. 12, 13-20 (2007).</li><li>Jin, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Establishment+of+three-dimensional+tissue-engineered+bone+constructs+under+microgravity-simulated+conditions.">Establishment of three-dimensional tissue-engineered bone constructs under microgravity-simulated conditions.</a> Artif Organs. 34, 118-125 (2010).</li><li>Vertrees, R. A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+a+three-dimensional+model+of+lung+cancer+using+cultured+transformed+lung+cells.">Development of a three-dimensional model of lung cancer using cultured transformed lung cells.</a> Cancer Biol Ther. 8, 356-365 (2009).</li><li>Hwang, Y. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+murine+embryonic+stem+cells,+alginate+encapsulation,+and+rotary+microgravity+bioreactor+in+bone+tissue+engineering.">The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering.</a> Biomaterials. 30, 499-507 (2009).</li><li>Pei, M., He, F., Kish, V. L., Vunjak-Novakovic, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+of+functional+cartilage+tissue+using+stem+cells+from+synovial+lining:+a+preliminary+study.">Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study.</a> Clin. Orthop Relat. Res. 466, 1880-1889 (2008).</li></ol>

قد الاستفادة من تكنولوجيا مفاعل حيوي RWV المقدمة هنا توفر للباحثين لديها القدرة على دفع ثقافتهم خلية النظام الحالي لأكثر من الناحية الفسيولوجية ذات الصلة عضوي النمط ثقافة نموذج الخلية. الخلية مفاعل حيوي RWV نظام ثقافة يوفر المكروية القص المنخفض التي تمكن الخلايا لتشك?...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> اسم كاشف </td><td> شركة </td><td> فهرس العدد </td><td> تعليقات </td></tr><tr><td> اليكسا فلور 488 </td><td> إينفيتروجن </td><td> A21131 </td><td> يستخدم في تخفيف 1:500 </td></tr><tr><td> FACSDiva </td><td> دينار بحريني </td><td></td><td> تدفق عداد الكريات </td></tr><tr><td> β-tublin الأضداد </td><td> Calbiochem </td><td> 654162 </td><td> يستخدم في تخفيف 1:5000 </td></tr><tr><td> الحيوي الصفيف 2000 </td><td> BioRad </td><td> 171-000205 </td><td> V5 البرمجيات </td></tr><tr><td> مفاعل حيوي ومكونات </td><td> Synthecon </td><td> المراكز المناخية الإقليمية-4 </td><td></td></tr><tr><td> خلية مصفاة </td><td> دينار بحريني الصقر </td><td> 352340 </td><td> حجم المسام 40μm </td></tr><tr><td> مخروطي أنبوب (50mL) </td><td> كورنينج </td><td> 5-538-60 </td><td></td></tr><tr><td> Coverslips </td><td> VWR </td><td> 48366067 </td><td></td></tr><tr><td> مجموعات خلوى مجموعة خرزة </td><td> BioRad </td><td> الإنسان عدة مخصصة </td><td></td></tr><tr><td> الخرز Cytodex </td><td> سيغما </td><td> C3275 </td><td></td></tr><tr><td> DPBS </td><td> Gibco </td><td> 14190 </td><td></td></tr><tr><td> EDTA </td><td> سيغما </td><td> ED-500G </td><td> Ethylenediaminetetraacetic حامض </td></tr><tr><td> الأجسام المضادة المحددة الظهارية (ESA) </td><td> Chemicon </td><td> CBL251 </td><td> تستخدم في الساعة 1:50 تخفيف </td></tr><tr><td> الجنين المصل البقري (FBS) </td><td> Gibco </td><td> 10438 </td><td> المعطل الحرارة </td></tr><tr><td> حارف (نيكل) </td><td> Synthecon </td><td> D-405 </td><td></td></tr><tr><td> حامض الهيدروكلوريك </td><td> سيغما </td><td> 258148 </td><td> 37٪ </td></tr><tr><td> Involucrin الأضداد </td&gt; <td> سيغما </td><td> أنا 9018 </td><td></td></tr><tr><td> مجهر الشرائح </td><td> VWR </td><td> 16004-368 </td><td></td></tr><tr><td> MTT الكاشف </td><td> النائب Biomedicals، ذ م م </td><td> 194592 </td><td> 3 - (4،5-Dimethylthiazolyl 1-2) بروميد Tetrazolium -2،5-ثنائي الفينيل </td></tr><tr><td> MUC1 الأجسام المضادة (المجهر) </td><td> سانتا كروز </td><td> SC-7313 </td><td> تستخدم في الساعة 1:50 تخفيف </td></tr><tr><td> MUC1 الأجسام المضادة (التدفق الخلوي) </td><td> دينار بحريني Pharmingen </td><td> 559774 </td><td> كما دعا CD227، استخدم 20μL لكل اختبار </td></tr><tr><td> بارافورمالدهيد </td><td> علوم المجهر الإلكتروني </td><td> 15710 </td><td> المخفف الى 4٪ في DPBS </td></tr><tr><td> طبق بيتري (صغير) </td><td> دينار بحريني الصقر </td><td> 353002 </td><td></td></tr><tr><td> أنبوب البوليسترين مع فلتر </td><td> دينار بحريني الصقر </td><td> 352235 </td><td></td></tr><tr><td&gt; أنبوب تدفق البوليستيرين </td><td> دينار بحريني الصقر </td><td> 352058 </td><td></td></tr><tr><td> العلاقات العامة الأضداد </td><td> DAKO </td><td> M3569 </td><td> يستخدم في تخفيف 1:100 </td></tr><tr><td> إطالة الذهب </td><td> إينفيتروجن </td><td> P36931 </td><td> تزايد وسائل الاعلام مع دابي </td></tr><tr><td> RNeasy البسيطة كيت </td><td> QIAGEN </td><td> 74903 </td><td></td></tr><tr><td> الصوديوم دوديسيل كبريتات </td><td> سيغما </td><td> 71725 </td><td></td></tr><tr><td> التعقيم الحقيبة </td><td> VWR </td><td> 11213-035 </td><td></td></tr><tr><td> الصمامات (في اتجاه واحد) </td><td> MEDEX </td><td> MX5061L </td><td></td></tr><tr><td> حقنة (10ML) </td><td> دينار بحريني </td><td> 309604 </td><td> Luer قفل غيض </td></tr><tr><td> حقنة (5mL) </td><td> دينار بحريني </td><td> 309603 </td><td> Luer قفل غيض </td></tr><tr><td> زرقة التريبان </td><td> إينفيتروجن </td><td>T10282 </td><td></td></tr><tr><td> Vp5 الأضداد </td><td> سانتا كروز </td><td> SC-13525 </td><td> HSV-2 استنساخ الضد 6F10، وتستخدم في تخفيف 1:5000 </td></tr></tbody></table>

culturing and applications of rotating wall vessel bioreactor derived 3d epithelial cell models

Cells and tissues in the body experience environmental conditions that influence their architecture, intercellular communications, and overall functions. For in vitro cell culture models to accurately mimic the tissue of interest, the growth environment of the culture is a critical aspect to consider. Commonly used conventional cell culture systems propagate epithelial cells on flat two-dimensional (2-D) impermeable surfaces. Although much has been learned from conventional cell culture systems, many findings are not reproducible in human clinical trials or tissue explants, potentially as a result of the lack of a physiologically relevant microenvironment.
Here, we describe a culture system that overcomes many of the culture condition boundaries of 2-D cell cultures, by using the innovative rotating wall vessel (RWV) bioreactor technology. We and others have shown that organotypic RWV-derived models can recapitulate structure, function, and authentic human responses to external stimuli similarly to human explant tissues 1-6. The RWV bioreactor is a suspension culture system that allows for the growth of epithelial cells under low physiological fluid shear conditions. The bioreactors come in two different formats, a high-aspect rotating vessel (HARV) or a slow-turning lateral vessel (STLV), in which they differ by their aeration source. Epithelial cells are added to the bioreactor of choice in combination with porous, collagen-coated microcarrier beads (Figure 1A). The cells utilize the beads as a growth scaffold during the constant free fall in the bioreactor (Figure 1B). The microenvironment provided by the bioreactor allows the cells to form three-dimensional (3-D) aggregates displaying in vivo-like characteristics often not observed under standard 2-D culture conditions (Figure 1D). These characteristics include tight junctions, mucus production, apical/basal orientation, in vivo protein localization, and additional epithelial cell-type specific properties.
The progression from a monolayer of epithelial cells to a fully differentiated 3-D aggregate varies based on cell type1, 7-13. Periodic sampling from the bioreactor allows for monitoring of epithelial aggregate formation, cellular differentiation markers and viability (Figure 1D). Once cellular differentiation and aggregate formation is established, the cells are harvested from the bioreactor, and similar assays performed on 2-D cells can be applied to the 3-D aggregates with a few considerations (Figure 1E-G). In this work, we describe detailed steps of how to culture 3-D epithelial cell aggregates in the RWV bioreactor system and a variety of potential assays and analyses that can be executed with the 3-D aggregates. These analyses include, but are not limited to, structural/morphological analysis (confocal, scanning and transmission electron microscopy), cytokine/chemokine secretion and cell signaling (cytometric bead array and Western blot analysis), gene expression analysis (real-time PCR), toxicological/drug analysis and host-pathogen interactions. The utilization of these assays set the foundation for more in-depth and expansive studies such as metabolomics, transcriptomics, proteomics and other array-based applications. Our goal is to present a non-conventional means of culturing human epithelial cells to produce organotypic 3-D models that recapitulate the human in vivo tissue, in a facile and robust system to be used by researchers with diverse scientific interests.

Watch this Scientific Journal Video about Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models at JoVE.com

Culturing and Applications of Rotating Wall Vessel Bioreactor Derived 3D Epithelial Cell Models

يوصف ثقافة الخلية الدورية نظام يسمح الخلايا الظهارية في النمو في ظل الظروف الفسيولوجية الناتجة في 3 مد تشكيل مجموع الخلوية. ولدت المجاميع العرض<em&gt; في الجسم الحي</em&gt; مثل الخصائص التي لم تشاهد في نماذج الثقافة التقليدية، ويكون بمثابة نموذج نظام عضوي النمط أكثر دقة للعديد من التحقيقات العلمية.

المستمدة زراعة والتطبيقات تناوب الحائط تفاعلات الاحيائية سفينة 3D نماذج الخلايا الظهارية

Cells and tissues in the body experience environmental conditions that influence their architecture, intercellular communications, and overall functions. ...

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JoVE Journal

Biology

Absolute Quantum Yield Measurement of Powder Samples

مطلق قياس العائد الكم من عينات مسحوق

Measurement of fluorescence quantum yield has become an important tool in the search for new solutions in the development, evaluation, quality control and ...

A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid

ونظام 3D لغضروفية التثقيف مفصلي الإنسان في السائل الزليلي

Cartilage destruction is a central pathological feature of osteoarthritis, a leading cause of disability in the US. Cartilage in the adult does not ...

Mouse Sperm Cryopreservation and Recovery using the I&middot;Cryo Kit

الماوس حفظ بالبرودة والانتعاش الحيوانات المنوية باستخدام · لقد كرو كيت

Thousands of new genetically modified (GM) strains of mice have been created since the advent of transgenesis and knockout technologies.  Many of these ...

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

غليكان التنميط من البوليمرات جدار الخلية النباتية باستخدام [ميكروأرس]

Plant cell walls are complex matrixes of heterogeneous glycans which play an important role in the physiology and development of plants and provide the ...

Culturing of Human Nasal Epithelial Cells at the Air Liquid Interface

زراعة خلايا الإنسان الظهارية الأنف في واجهة الهواء السائل

In vitro models using human primary  epithelial cells are essential in understanding key functions of the respiratory  epithelium in the context of ...

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

العزلة من الدم والأوعية المشتقة متعددة القدرات السلائف من العضلات الهيكلية البشرية

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization&#160;of MSCs have been obscured by their retrospective ...

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements

تلطيخ النسيجية من<em&gt; نبات الأرابيدوبسيس thaliana</em&gt; الخليوي الثانوية ستريت عناصر

Arabidopsis thaliana is a model organism commonly used to understand and manipulate various cellular processes in plants, and it has been used extensively ...

Subretinal Transplantation of Human Embryonic Stem Cell Derived-retinal Pigment Epithelial Cells into a Large-eyed Model of Geographic Atrophy

سوبريتينال زرع الخلايا الظهارية الصبغية الشبكية المستمدة من الخلايا الجذعية الجنينية البشرية إلى نموذج كبير العينين من ضمور الجغرافية

Geographic atrophy (GA), the late stage of dry age-related macular degeneration is characterized by loss of the retinal pigment epithelial (RPE) layer, ...

Application of Membrane and Cell Wall Selective Fluorescent Dyes for Live-Cell Imaging of Filamentous Fungi

تطبيق غشاء وجدار الخلية الاصباغ الفلورية الانتقائية لتصوير الخلايا الحية من الفطريات الخيطية

The application of membrane and cell wall selective fluorescent dyes for live-cell imaging analyses of organelle dynamics in fungal cells started two ...

3D Culturing of Organoids from the Intestinal Villi Epithelium Undergoing Dedifferentiation

3D زراعة الأجهزة العضوية من ظهارة سيلي المعوية التي تخضع للتمايز

Clonogenicity of organoids from the intestinal epithelium is attributed to the presence of stem cells therein. The mouse small intestinal epithelium is ...