Name: phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood
Uploaded: 2012-04-13T13:00:00
Description: Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

博士YoderはEndGenitor Technologies社とRimedion Technologies社の取締役会のメンバーへのコンサルタントです。

試薬およびソリューション EMG-2メディア（ロンザ、カタログ番号CC-3162 EBM-2培地とEGM-2 SingleQuotキットサプリメント＆成長因子を含む） 全体SingleQuotキットサプリメント＆成長因子（ロンザ、カタログ番号CC-4176）、10％（v / v）のウシ胎児血清（FBS）を添加したEBM-2（ロンザ、カタログ番号CC-3156）と1％（v / v）のペニシリン（10,000 U / ml）を/ストレプトマイ?...

<ol>
	<li>Carmeliet, P., Jain, R. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+mechanisms+and+clinical+applications+of+angiogenesis.">Molecular mechanisms and clinical applications of angiogenesis.</a> Nature. 473, 298-307 (2011).</li><li>Asahara, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+of+putative+progenitor+endothelial+cells+for+angiogenesis.">Isolation of putative progenitor endothelial cells for angiogenesis.</a> Science. 275, 964-967 (1997).</li><li>Urbich, C., Dimmeler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endothelial+progenitor+cells:+characterization+and+role+in+vascular+biology.">Endothelial progenitor cells: characterization and role in vascular biology.</a> Circ. Res. 95, 343-353 (2004).</li><li>Critser, P. J., Voytik-Harbin, S. L., Yoder, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolating+and+defining+cells+to+engineer+human+blood+vessels.">Isolating and defining cells to engineer human blood vessels.</a> Cell. Prolif. 44, 15-21 (2011).</li><li>Matthias, M., David, N., Josef, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=From+bench+to+bedside:+what+physicians+need+to+know+about+endothelial+progenitor+cells.">From bench to bedside: what physicians need to know about endothelial progenitor cells.</a> Am. J. Med. 124, 489-4897 (2011).</li><li>Ingram, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vessel+wall-derived+endothelial+cells+rapidly+proliferate+because+they+contain+a+complete+hierarchy+of+endothelial+progenitor+cells.">Vessel wall-derived endothelial cells rapidly proliferate because they contain a complete hierarchy of endothelial progenitor cells.</a> Blood. 105, 2783-276 (2005).</li><li>Ingram, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+a+novel+hierarchy+of+endothelial+progenitor+cells+using+human+peripheral+and+umbilical+cord+blood.">Identification of a novel hierarchy of endothelial progenitor cells using human peripheral and umbilical cord blood.</a> Blood. 104, 2752-2760 (2004).</li><li>Yoder, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Redefining+endothelial+progenitor+cells+via+clonal+analysis+and+hematopoietic+stem/progenitor+cell+principals.">Redefining endothelial progenitor cells via clonal analysis and hematopoietic stem/progenitor cell principals.</a> Blood. 109, 1801-1809 (2007).</li><li>Reinisch, A., Strunk, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+Animal+Serum+Free+Expansion+of+Human+Umbilical+Cord+Derived+Mesenchymal+Stromal+Cells+(MSCs)+and+Endothelial+Colony+Forming+Progenitor+Cells+(ECFCs.">Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs.</a> J. Vis. Exp. (32), e1525 (2009).</li><li>Au, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+in+vivo+potential+of+endothelial+progenitor+cells+from+human+umbilical+cord+blood+and+adult+peripheral+blood+to+form+functional+long-lasting+vessels.">Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels.</a> Blood. 111, 1302-135 (2008).</li><li>Critser, P. J., Kreger, S. T., Voytik-Harbin, S. L., Yoder, M. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Collagen+matrix+physical+properties+modulate+endothelial+colony+forming+cell-derived+vessels+in+vivo.">Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo.</a> Microvasc. Res. 80, 23-30 (2010).</li><li>Melero-Martin, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+robust+and+functional+vascular+networks+in+vivo+with+human+adult+and+cord+blood-derived+progenitor+cells.">Engineering robust and functional vascular networks in vivo with human adult and cord blood-derived progenitor cells.</a> Circ. Res. 103, 194-202 (2008).</li><li>Melero-Martin, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+vasculogenic+potential+of+human+blood-derived+endothelial+progenitor+cells.">In vivo vasculogenic potential of human blood-derived endothelial progenitor cells.</a> Blood. 109, 4761-4768 (2007).</li><li>Hofmann, N. A., Reinisch, A., Strunk, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+Large+Scale+Expansion+of+Adult+Human+Endothelial+Colony+Forming+Progenitor+Cells.">Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells.</a> J. Vis. Exp. (32), e1524 (2009).</li><li>Lin, Y., Weisdorf, D. J., Solovey, A., Hebbel, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Origins+of+circulating+endothelial+cells+and+endothelial+outgrowth+from+blood.">Origins of circulating endothelial cells and endothelial outgrowth from blood.</a> J. Clin. Invest. 105, 71-77 (2000).</li><li>Witting, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficient+Large+Volume+Lentiviral+Vector+Production+Using+Flow+Electroporation.">Efficient Large Volume Lentiviral Vector Production Using Flow Electroporation.</a> Hum. Gene. Ther. , (2011).</li><li>Yoon, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synergistic+neovascularization+by+mixed+transplantation+of+early+endothelial+progenitor+cells+and+late+outgrowth+endothelial+cells:+the+role+of+angiogenic+cytokines+and+matrix+metalloproteinases.">Synergistic neovascularization by mixed transplantation of early endothelial progenitor cells and late outgrowth endothelial cells: the role of angiogenic cytokines and matrix metalloproteinases.</a> Circulation. , 112-1618 (2005).</li><li>Dubois, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+effects+of+progenitor+cell+populations+on+left+ventricular+remodeling+and+myocardial+neovascularization+after+myocardial+infarction.">Differential effects of progenitor cell populations on left ventricular remodeling and myocardial neovascularization after myocardial infarction.</a> J. Am. Coll. Cardiol. 55, 2232-2243 (2010).</li><li>Medina, R. J., O'Neill, C. L., Humphreys, M. W., Gardiner, T. A., Stitt, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Outgrowth+endothelial+cells:+characterization+and+their+potential+for+reversing+ischemic+retinopathy.">Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathy.</a> Invest. Ophthalmol. Vis. Sci. 51, 5906-5913 (2010).</li></ol>

推定される内皮前駆細胞の表現型および機能解析は、クローンと連続して再メッキ文化の中で可能である善意の ECFCsを識別し、in vivoでの耐久性と機能的な植込み型血管を生じさせることが重要です。ヒト臍帯血はECFCsと老化や病気の10のこれらの循環細胞の減少の濃度で濃縮されています。最近の研究では、ECFCは、血管損傷、心筋梗塞、網膜症17から19までの状?...

<table border="1"><tbody><tr><td> 試薬の名前 </td><td> 会社 </td><td> カタログ番号 </td></tr><tr><td>ヘパリンナトリウム注射液、USP </td><td> APPファーマシューティカル</td><td> 504031 </td></tr><tr><td>フィコール - Pague </td><td>アマシャムバイオサイエンス</td><td> 17-1440-03 </td></tr><tr><td>ミキシングカニューレ</td><td>マースク医療</td><td> 500.11.012 </td></tr><tr><td> EGM-2 </td><td>ロンザ</td><td> CC-3162 </td></tr><tr><td>定義されたFBS </td><td>ハイクローン</td><td> SH30070.03 </td></tr><tr><td> TrypLE急行</td><td>ギブコ</td><td> 12605 </td></tr><tr><td>ラットI型コラーゲン</td><td> BDバイオサイエンス</td><td> 354236 </td></tr><tr><td>マトリゲル</td><td> BDバイオサイエンス</td><td> 356234 </td></tr><tr><td> FcRをブロック</td><td>ミルテニーバイオテク</td><td> 130-059-901 </td></tr><tr><td> hCD31、FITC抱合テッド</td><td> BD Pharmingen社</td><td> 555445 </td></tr><tr><td> hCD45、FITCコンジュゲート</td><td> BD Pharmingen社</td><td> 555482 </td></tr><tr><td> hCD14、FITCコンジュゲート</td><td> BD Pharmingen社</td><td> 555397 </td></tr><tr><td> hCD144、PEコンジュゲート</td><td>ベイバイオサイエンス</td><td> 12-1449-80 </td></tr><tr><td> hCD146、PEコンジュゲート</td><td> BD Pharmingen社</td><td> 550315 </td></tr><tr><td> hCD105、PEコンジュゲート</td><td>インビトロジェン</td><td> MHCD10504 </td></tr><tr><td> MSのIgG 1、K抗体、FITCコンジュゲート</td><td> BD Pharmingen社</td><td> 555748 </td></tr><tr><td> MSのIgG 1、K抗体、PEコンジュゲート</td><td> BD Pharmingen社</td><td> 559320 </td></tr><tr><td> MSのIgG 2aは、K抗体、FITCコンジュゲート</td><td> BD Pharmingen社</td><td> 555573 </td></tr><tr><td>抗ヒトCD31 </td><td> Dako社</td><td>クローンJC70 / </td></tr><tr><td>アンチ覚書電子CD31 </td><td> BD Pharmingen社</td><td> 553370 </td></tr><tr><td> 0.22-μmの真空ろ過システム</td><td>ミリポア</td><td> SCGPU05RE </td></tr><tr><td>氷酢酸、17.4N </td><td>フィッシャー</td><td> A38-500 </td></tr><tr><td>抗生物質抗真菌</td><td>インビトロジェン</td><td> 15240-062 </td></tr><tr><td>ウシ胎児血清（FBS） </td><td>ハイクローン</td><td> SH30070.03 </td></tr><tr><td> IHC亜鉛固定液</td><td> BDバイオサイエンス</td><td> 550523 </td></tr><tr><td> SYTOXグリーン試薬</td><td>インビトロジェン</td><td> S33025 </td></tr><tr><td>滅菌クローニングシリンダー、 </td><td>フィッシャー·サイエンティフィック</td><td> 07-907-10 </td></tr></tbody></table>

phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood

血管新生、脈管形成、および動脈形成を介して新しい血管の形成の長年のビューは、最近1日されています。内皮前駆細胞（EPC）の循環の存在が最初に麻原らが成人ヒト末梢血で同定された1997年2血管の再生と修理のための新しい仮説と戦略の注入をもたらすインチEPCは血管形成または血管リモデリングのサイトへのホームという血液の循環まれではあるが、通常のコンポーネントであり、主にセル3派生した既存の血管壁のパラクリン刺激を介して出生後の脈管形成、血管新生、または動脈形成のいずれかを容易にします。 EPCを識別するために、特定のマーカーが同定されず、フィールドの現時点での状態は、血管新生、造血幹細胞や前駆細胞を含め、その多くの細胞タイプを理解することであり、新生細胞、Tie2の+単球、骨髄前駆セルを循環されていますlsは、腫瘍関連マクロファージ、M2活性化マクロファージは、前臨床動物モデル系の様々な、多くの病気の状態4、5、人間の被験者の血管新生のプロセスを刺激するに参加しています。内皮コロニー形成細胞（ECFCs）堅牢なクローン増殖能、播種時に能力を形成する第二級および第三級コロニー、および免疫不全マウスへの移植時に6月8日 in vivoで血管内に本質的な形成する能力によって特徴付けまれな循環可能な内皮細胞である。 ECFCsが正常健康成人被験者、健康な新生児の臍帯血（CB）、および多数の人間の動脈および静脈の血管6-9の血管壁の末梢血から単離されているが、CBはECFCs 7の最高周波数を有していることを表示最も堅牢なクローン増殖能および in vivo 8、10-13 のフォーム耐久性と機能的な血管を。 whileの導出成人の末梢血からECFCここでは、我々が導出するための方法論を記述し、14、15を提示されており、クローニング、拡張、およびin vitroで同様にヒト臍帯CBからECFCs の in vivo特性のように 。

Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

内皮コロニー形成細胞（ECFCs）組み込み表示されていること、堅牢なクローン増殖能と血管内皮細胞を循環している<emin vivoでの&gt;</em&gt;船が能力を形成する。 CBから派生した副産物内皮細胞の表現型および機能解析を識別し、分離することが重要です。<em善意&gt;善意</em損傷した組織を修復するの潜在的な臨床応用のために&gt; ECFCs。

ヒト臍帯血由来血管内皮コロニー形成細胞の表現型および機能解析

Longstanding views of new blood vessel formation via angiogenesis, vasculogenesis, and arteriogenesis have been recently reviewed1. The presence of ...

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Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

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This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult ...

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ヒト臍帯由来間葉系幹細胞（MSCs）と血管内皮コロニー形成前駆細胞（ECFCs）の単離および動物血清自由膨張

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Endothelial cells provide a useful research model in many areas of vascular biology. Since its first isolation 1, human umbilical vein endothelial cells ...

A Quantitative Assay for Insulin-expressing Colony-forming Progenitors

インスリン発現コロニーを形成する前駆細胞の定量アッセイ

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人間の脂肪吸引物からの脂肪由来幹細胞を手動で隔離

In 2001, researchers at the University of  California, Los Angeles, described the isolation of a new population of adult  stem cells from liposuctioned ...

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ヒト骨格筋からの血管由来の多能前駆体の単離

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization&#160;of MSCs have been obscured by their retrospective ...

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

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細胞内カルシウムの二光子イメージング<sup&gt; 2+</sup&gt;取扱い及び内皮における一酸化窒素産生および単離されたラット大動脈の平滑筋細胞

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