Name: phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood
Uploaded: 2012-04-13T13:00:00
Description: Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

박사 Yoder는 EndGenitor 기술 주식 회사에 컨설턴트와 Rimedion 테크놀로지 주식 회사의 이사회의 구성원입니다

시약 및 솔루션 EMG-2 미디어 (Lonza, 고양이. 번호 CC-3162 EBM-2 기초 미디엄과 EGM-2 SingleQuot 키트 보충, 및 성장 요인을 포함) EBM-2 (Lonza, 고양이. 번호 CC-3156)는 전체 SingleQuot 키트 보충 및 성장 요인과 보충 (Lonza, 고양이. 번호 CC-4176), 10 % (V / V) 태아 소 혈청 (FBS) 및 1% (V / V) 페니실린 (10,000 U / ml) 쇼핑 / 스트렙토 마이신 (10,000 μg / ml) 쇼핑 / amphotericin (25 μg / ml) 쇼핑....

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putative 내피 전구 세포의 Phenotypic 및 기능적 특성은 clonally하고 순차적으로 다시 도금 문화에 능력이있다 타고난 ECFCs를 파악하고 생체내의 내구성과 기능성 implantable 혈관을 야기할 것이 중요합니다. 인간의 탯줄 혈액은 ECFCs 및 노화 또는 질병 10 이러한 순환 세포 감소의 농도로 풍부합니다. 최근 연구 ECFC은 혈관 상해, 심근 경색, 또는 망막 병증 17-19의 상황에서 혈?...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> 헤파린 나트륨 주입, USP </td><td> APP 제약 </td><td> 504,031 </td></tr><tr><td> Ficoll-Pague </td><td> Amersham Biosciences </td><td> 17-1440-03 </td></tr><tr><td> 혼합 정맥 </td><td> Maersk 의료 </td><td> 500.11.012 </td></tr><tr><td> EGM-2 </td><td> Lonza </td><td> CC-3162 </td></tr><tr><td> 정의 FBS </td><td> Hyclone </td><td> SH30070.03 </td></tr><tr><td> TrypLE 급행 </td><td> Gibco </td><td> 12,605 </td></tr><tr><td> 쥐 종류 나 콜라겐 </td><td> BD Biosciences </td><td> 354,236 </td></tr><tr><td> Matrigel </td><td> BD Biosciences </td><td> 356,234 </td></tr><tr><td> FcR 블록 </td><td> Miltenyi 생명 공학 </td><td> 130-059-901 </td></tr><tr><td> hCD31, FITC conjuga널어서 말리다 </td><td> BD Pharmingen </td><td> 555,445 </td></tr><tr><td> hCD45, FITC 복합 </td><td> BD Pharmingen </td><td> 555,482 </td></tr><tr><td> 복합 hCD14, FITC </td><td> BD Pharmingen </td><td> 555,397 </td></tr><tr><td> hCD144, PE 복합 </td><td> eBioscience </td><td> 12-1449-80 </td></tr><tr><td> hCD146, PE 복합 </td><td> BD Pharmingen </td><td> 550,315 </td></tr><tr><td> hCD105, PE 복합 </td><td> Invitrogen </td><td> MHCD10504 </td></tr><tr><td> MS IgG 1, K 항체, 복합 FITC </td><td> BD Pharmingen </td><td> 555,748 </td></tr><tr><td> MS IgG 1, K 항체, 복합 체육 </td><td> BD Pharmingen </td><td> 559,320 </td></tr><tr><td> MS IgG 2A, K 항체, 복합 FITC </td><td> BD Pharmingen </td><td> 555,573 </td></tr><tr><td> 안티 인간 CD31 </td><td> Dako </td><td> 클론 JC70 / </td></tr><tr><td> 안티 mous전자 CD31 </td><td> BD Pharmingen </td><td> 553,370 </td></tr><tr><td> 0.22-μm의 진공 여과 시스템 </td><td> Millipore </td><td> SCGPU05RE </td></tr><tr><td> 빙초산, 17.4N </td><td> 어부 </td><td> A38-500 </td></tr><tr><td> 항생제 - Antimycotic </td><td> Invitrogen </td><td> 15240-062 </td></tr><tr><td> 태아 소 혈청 (FBS) </td><td> Hyclone </td><td> SH30070.03 </td></tr><tr><td> IHC 아연 정착액 </td><td> BD Biosciences </td><td> 550,523 </td></tr><tr><td> Sytox 녹색 시약 </td><td> Invitrogen </td><td> S33025 </td></tr><tr><td> 멸균 복제 실린더, </td><td> 피셔 과학 </td><td> 07-907-10 </td></tr></tbody></table>

phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood

angiogenesis, vasculogenesis 및 arteriogenesis 통해 새로운 혈관 형성의 오랜 전망은 최근 1을 검토하고있다. 내피 전구 세포 (EPCs)를 순환의 존재가 처음 Asahara 외 의해 성인이 인간의 말초 혈액에서 발견되었다. 1,997 2가 혈관 재생 및 수리에 대한 새로운 가설과 전략의 주입을 가져 인치 EPCs은 혈액 순환의 드물지만, 일반적인 구성 요소입니다 그 혈관 형성이나 혈관 리모델링의 사이트에 대한 가정, 그리고 세포 3 파생 기존 선박 벽 paracrine 자극을 통해 주로 중 출생 후의 vasculogenesis, angiogenesis, 또는 arteriogenesis을 용이하게합니다. EPC를 식별하기위한 특별한 표지가 식별하지 않으며, 필드의 현재 상태는 proangiogenic 조혈 줄기 및 전구 세포를 포함한 그 많은 세포 유형을 이해하고, angiogenic 세포, Tie2 + monocytes, 골수양 전구 cel을 순환되었습니다혹시, 종양 관련 macrophages, 그리고 M2 활성화 macrophages는 잠복기 동물 모델 시스템의 다양한 수많은 질병 상태 4, 5의 인간 과목 angiogenic 과정을 자극에 참여합니다. 내피 콜로니 형성 세포 (ECFCs) 강력한 clonal proliferative 가능성 replating시 능력을 형성 보조와 차 식민지, 그리고 immunodeficient 생쥐 6-8로 이식시 생체내 혈관 형성에서 본질적인 능력을 특징으로 희귀 순환 가능한 내피 세포 수 있습니다. ECFCs이 성공적으로 건강한 성인 과목, 건강한 신생아 유아의 탯줄 혈액 (CB), 그리고 수많은 인간의 동맥과 정맥 혈관 6-9의 혈관 벽의 말초 혈액으로부터 격리되어 있지만, CB는 ECFCs 7의 높은 주파수를 보유하고 그 표시 가장 강력한 clonal proliferative 잠재력과 생체내 8, 10-13의 형태로 내구성과 기능성 혈관을. 동안의 유도성인 말초 혈액에서 ECFC 여기서 우리가 파생을위한 방법론을 설명하는, 14, 15를 발표되었으며, 복제, 확장, 그리고 체외에서뿐만 아니라 인간의 탯줄 CB에서 ECFCs의 생체내 특성에 있습니다.

Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

내피 콜로니 형성 세포 (ECFCs) 본질을 표시하는 강력한 clonal proliferative 잠재력과 내피 세포를 순환하고 있습니다<em&gt; 생체내에서</em&gt; 함선 능력을 형성. CB에서 파생된 가지 내피 세포의 Phenotypic 및 기능적 특성 식별하고 격리하는 데 중요<em거짓&gt; 어딘가에서</em&gt; 손상된 조직을 복구에서 잠재적인 임상 응용을위한 ECFCs.

내피 콜로니 형성 세포의 Phenotypic 및 기능성 특성은 인간 탯줄의 혈액에서 유래

Longstanding views of new blood vessel formation via angiogenesis, vasculogenesis, and arteriogenesis have been recently reviewed1. The presence of ...

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Biology

Isolation of Human Umbilical Vein Endothelial Cells (HUVEC)

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인간 제대 베인 내피 세포의 절연 (HUVEC)

Angiogenesis is a complex multi-step process, where in response to angiogenic stimuli, new vessels are created from the existing vasculature. These steps ...

연구

생물학

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

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This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult ...

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인간 탯줄의 분리 및 동물 혈청 무료 확장 Mesenchymal Stromal 세포 (MSCS)와 내피 콜로니 형성 전구 세포 (ECFCs)을 유래

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The field of pancreatic stem and progenitor cell biology has been hampered by a lack of in vitro functional and quantitative assays that allow for the ...

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In 2001, researchers at the University of  California, Los Angeles, described the isolation of a new population of adult  stem cells from liposuctioned ...

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Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization&#160;of MSCs have been obscured by their retrospective ...

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세포 내 칼슘의 두 광자 이미징<sup&gt; 2 +</sup&gt; 고립 된 쥐 대동맥의 취급 및 내피 세포에서 산화 질소 생산과 부드러운 근육 세포

Calcium is a very important regulator of many physiological processes in vascular tissues. Most endothelial and smooth muscle functions highly depend on ...

Generation and Culture of Blood Outgrowth Endothelial Cells from Human Peripheral Blood

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Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular ...