Name: phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood
Uploaded: 2012-04-13T13:00:00
Description: Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

Dr. Yoder er konsulent for EndGenitor Technologies, Inc. og et medlem av styret i Rimedion Technologies, Inc.

Reagenser og løsninger EMG-2-medier (Lonza, Kat. Nr. cc-3162 inneholder EBM-2 basal medium og EGM-2 SingleQuot kit kosttilskudd, og vekstfaktorer) EBM-2 (Lonza, Cat. Nr. cc-3156) supplert med hele SingleQuot kit kosttilskudd og vekstfaktorer (Lonza, Kat. Nr. cc-4176), 10% (v / v) fosteret storfe serum (FBS) og 1% (v / v) penicillin (10 000 U / ml) / streptomycin (10.000 mikrogram / ml) / amfotericin (25 mikrogram / ml). Lagre opptil 1 måned ved 4 ° C. Anbefalt EGM-...

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Fenotypisk og funksjonell karakterisering av antatte endoteliale progenitorceller er viktig å identifisere de godtroende ECFCs som er i stand clonally og serielt re-plating i kultur og gi opphav til holdbare og funksjonelle implanterbare blodkar in vivo. Menneskelig navlestrengsblod er beriket med ECFCs og konsentrasjonen av disse sirkulerende celler avtar med aldring eller sykdom 10. Nyere studier tyder på at ECFC kan spille viktige roller i vaskulær reparasjon eller fornyelse i situasjo...

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phenotypic and functional characterization of endothelial colony forming cells derived from human umbilical cord blood

Langvarig utsikt over nye blodkar dannes via angiogenese, vasculogenesis, og arteriogenesis har nylig blitt anmeldt en. Tilstedeværelsen av sirkulerende endoteliale progenitorceller (EPCs) ble først identifisert i voksent menneske perifert blod ved Asahara et al. I 1997 to bringe en infusjon av nye hypoteser og strategier for vaskulær fornyelse og reparasjon. EPCs er sjeldne, men normale komponentene i sirkulerende blod som hjem til nettsteder av blodkar dannes eller vaskulær remodellering, og legge til rette enten postnatal vasculogenesis, angiogenese, eller arteriogenesis hovedsak via parakrine stimulering av eksisterende åreveggen avledet celler 3. Ingen spesifikk markør for å identifisere en EPC har blitt identifisert, og i dag staten av feltet er å forstå at en rekke celletyper, inkludert proangiogenic hematopoietisk stem og stamceller, sirkulerende angiogenic celler, Tie2 + monocytter, myeloid progenitor Cells, tumor assosiert makrofager, og M2 aktiverte makrofager delta i å stimulere angiogenic prosessen i en rekke prekliniske dyremodell systemer og i mennesker i en rekke sykdomstilstander 4, 5. Endoteliale colony forming celler (ECFCs) er sjeldne sirkulerende levedyktige endotelceller preget av robuste klonal proliferativ potensial, sekundær og tertiær koloni forming evne på replating, og evne til å danne indre in vivo skip upon transplantasjon inn immunsvikt mus 6-8. Mens ECFCs har blitt isolert fra perifert blod hos friske voksne personer, navlestreng blod (CB) av friske nyfødte, og åreveggen av mange menneskelige arterielle og venøse fartøy 6-9, besitter CB den høyeste frekvensen av ECFCs 7 som skjerm den mest robuste klonal proliferativ potensial og form holdbare og funksjonelle blodkar i 8 vivo, 10-13. Mens avledning avECFC fra voksen perifert blod har blitt presentert 14, 15, her vi beskrive metoder for avledning, kloning, ekspansjon, og in vitro, samt in vivo karakterisering av ECFCs fra den menneskelige umbilical CB.

Watch this Scientific Journal Video about Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood at JoVE.com

Phenotypic and Functional Characterization of Endothelial Colony Forming Cells Derived from Human Umbilical Cord Blood

Endoteliale colony forming celler (ECFCs) er sirkulerende endotelceller med robust klonal proliferativ potensial som viser iboende<em&gt; In vivo</em&gt; Fartøy forming evne. Fenotypisk og funksjonell karakterisering av utvekst endotelceller avledet fra CB er viktig å identifisere og isolere<em&gt; Bona fide</em&gt; ECFCs for potensiell klinisk anvendelse i å reparere skadet vev.

Fenotypisk og Funksjonell Karakterisering av endoteliale Colony forming celler avledet fra humant navlestrengsblod

Longstanding views of new blood vessel formation via angiogenesis, vasculogenesis, and arteriogenesis have been recently reviewed1. The presence of ...

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