Name: using eggs from schistosoma mansoni as an in vivo model of helminth-induced lung inflammation
Uploaded: 2012-06-05T13:00:00
Description: Watch this Scientific Journal Video about Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation at JoVE.com

这项工作是由美国国立卫生研究院（NIH）AI091759和克隆氏病和结肠炎基金会美国的威廉·谢尔比参数及家庭基金会研究奖（MN）的支持。 NIAID的血吸虫资源中心（NIAID的合同N01，A130026）提供的血吸虫感染小鼠。

1。净化血吸虫卵<ol><li>瑞士韦伯斯特小鼠感染血吸虫尾蚴，感染血吸虫生命周期8阶段。另外，获得NIAID的血吸虫病资源中心（血吸虫感染小鼠<a href="http://www.schisto-resource.org/" target="_blank">http://www.schisto-resource.org/~~V</a> ）。离开小鼠感染后6-7周，时间点，鸡蛋是在肝脏中，可以恢复如下所述（ 见图1）。 </li><li>安乐死与CO 2的小鼠，并用70％乙醇湿。将鼠?...

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	<li>World Health Organization. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prevention+and+control+of+schistosomiasis+and+soil-transmitted+helminthiasis:+report+of+a+WHO+expert+committee.">Prevention and control of schistosomiasis and soil-transmitted helminthiasis: report of a WHO expert committee.</a> WHO Technical Report Series 912. , (2002).</li><li>Pearce, E. J., MacDonald, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+immunobiology+of+schistosomiasis.">The immunobiology of schistosomiasis.</a> Nat. Rev. Immunol. 2, 499-511 (2002).</li><li>Sandler, N. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Global+gene+expression+profiles+during+acute+pathogen-induced+pulmonary+inflammation+reveal+divergent+roles+for+Th1+and+th2+responses+in+tissue+repair.">Global gene expression profiles during acute pathogen-induced pulmonary inflammation reveal divergent roles for Th1 and th2 responses in tissue repair.</a> J. Immunol. 171, 3655-3667 (2003).</li><li>Perrigoue, J. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=IL-31-IL-31R+interactions+negatively+regulate+type+2+inflammation+in+the+lung.">IL-31-IL-31R interactions negatively regulate type 2 inflammation in the lung.</a> J. Exp. Med. 204, 481-487 (2007).</li><li>Sandor, M., Weinstock, J. V., Wynn, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Granulomas+in+schistosome+and+mycobacterial+infections:+a+model+of+local+immune+responses.">Granulomas in schistosome and mycobacterial infections: a model of local immune responses.</a> Trends Immunol. 24, 44-52 (2003).</li><li>Nair, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alternatively+activated+macrophage-derived+RELM-{alpha}+is+a+negative+regulator+of+type+2+inflammation+in+the+lung.">Alternatively activated macrophage-derived RELM-{alpha} is a negative regulator of type 2 inflammation in the lung.</a> J. Exp. Med. 206, 937-952 (2009).</li><li>Dalton, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+method+for+the+isolation+of+schistosome+eggs+and+miracidia+free+of+contaminating+host+tissues.">A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues.</a> Parasitology. 115, 29-32 (1997).</li><li>Lewis, F. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Large-scale+laboratory+maintenance+of+Schistosoma+mansoni,+with+observations+on+three+schistosome/snail+host+combinations.">Large-scale laboratory maintenance of Schistosoma mansoni, with observations on three schistosome/snail host combinations.</a> J. Parasitol. 72, 813-829 (1986).</li><li>Tilney, N. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Patterns+of+lymphatic+drainage+in+the+adult+laboratory+rat.">Patterns of lymphatic drainage in the adult laboratory rat.</a> J. Anat. 109 (Pt. 3), 369-383 (1971).</li><li>Lewis, F., Coligan, J. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Schistosomiasis.">Schistosomiasis.</a> Current protocols in immunology. Chapter 19, Unit 19 (2001).</li><li>Kaplan, M. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Th2+cells+are+required+for+the+Schistosoma+mansoni+egg-induced+granulomatous+response.">Th2 cells are required for the Schistosoma mansoni egg-induced granulomatous response.</a> J. Immunol. 160, 1850-1856 (1998).</li><li>Wynn, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fibrotic+disease+and+the+T(H)1/T(H)2+paradigm.">Fibrotic disease and the T(H)1/T(H)2 paradigm.</a> Nat. Rev. Immunol. 4, 583-594 (2004).</li><li>Cheever, A. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variation+of+hepatic+fibrosis+and+granuloma+size+among+mouse+strains+infected+with+Schistosoma+mansoni.">Variation of hepatic fibrosis and granuloma size among mouse strains infected with Schistosoma mansoni.</a> Am. J. Trop. Med. Hyg. 37, 85-97 (1987).</li></ol>

在这里，介绍一个模型，采用血吸虫卵诱导2型肺部发炎。这种模式的典型特征包括强有力的Th2型免疫反应，气道炎症和肺肉芽肿形成。由于这些参数依赖CD4 Th2细胞的反应11，这是一个有用的模型，探讨Th2细胞反应的特定蛋白质或特定谱系缺失的影响。这里的读数将包括抗原特异性Th2细胞因子的量化分析，呼吸道细胞浸润，肺肉芽肿的大小。一个额外的参数，可衡量的是鸡蛋引起的?...

<table border="1"><tbody><tr><td> 试剂 </td><td> 尺寸/描述 </td><td> 公司 </td><td> 产品编号 </td></tr><tr><td>胶原酶/ Dispase酶</td><td> 500毫克</td><td>罗氏应用科学部</td><td> 11 097 113 001 </td></tr><tr><td>变化快客赫马3染色</td><td>收拾</td><td>尔科技有限公司有限责任公司</td><td> 122-911 </td></tr><tr><td> DMEM培养液</td><td>液体</td><td> Gibco公司</td><td> 11965 </td></tr><tr><td>猎鹰50毫升</td><td>收拾</td><td>鹘</td><td> 352070 </td></tr><tr><td> intramedic聚乙烯管</td><td> 0.58毫米</td><td>碧迪公司</td><td> 427410 </td></tr><tr><td>异氟醚</td><td> 250毫升</td><td>住持动物健康</td><td> 52600405 </td></tr><tr><td>氯胺酮</td><td> 100毫克/毫升</td><td>道奇堡，动物健康</TD&gt; <td> N / A </td></tr><tr><td>甘露糖受体抗体</td><td>素</td><td> ABD Serotec公司</td><td> MCA2235B </td></tr><tr><td>金属过滤器</td><td>标准厨房过滤器</td><td>目标</td><td>劳伦蒂斯</td></tr><tr><td>多聚甲醛</td><td> 16％ </td><td>电子Microsc。科学</td><td> 15710 </td></tr><tr><td>青霉素/ </td><td> 100X </td><td> Gibco公司</td><td> 15070 </td></tr><tr><td> Percoll密度</td><td> 1公升</td><td> GE医疗生物科学</td><td> 17 0891 01 </td></tr><tr><td> RELMα抗体</td><td> 50微克</td><td> PeproTech公司</td><td> 500-P214 </td></tr><tr><td>手术主题</td><td> 2.0美国药典100坪</td><td>公司外科专业</td><td> SP118 </td></tr><tr><td>针头</td><td> 23克，3/4英寸</td><td> BD真空采血管实验室。 MED。 </td><td>305143 </td></tr><tr><td>美国标准试验筛</td><td> 150微米，0.0059“ </td><td> WS泰勒公司</td><td> ASTME-11，规格。第100号</td></tr><tr><td>甲苯噻嗪</td><td> 20毫克/毫升</td><td>男管家</td><td> N / A </td></tr></tbody></table>

using eggs from schistosoma mansoni as an in vivo model of helminth-induced lung inflammation

血吸虫寄生虫血吸虫感染估计有200万人，全世界1。在血吸虫 ，严重的病理，包括肝纤维化和脾肿大，慢性感染引起的免疫反应，而不是寄生虫本身2寄生虫卵。寄生虫卵诱导Th2反应特点是生产的IL-4，IL-5和IL-13，替代激活的巨噬细胞和嗜酸性粒细胞的招聘。在这里，我们描述了一个模型来研究特定的寄生虫在Th2细胞因子反应和肺淋巴结，形成肺肉芽肿周围的蛋，呼吸道炎症注射血吸虫卵。 腹腔宣传和静脉的挑战， 与血吸虫卵被运送到肺，通过他们被困在肺实质的肺动脉由淋巴细胞，嗜酸性粒细胞和另外激活的巨噬细胞3-6组成的肉芽肿。与肉芽肿形成，在炎症的支气管肺泡空间关联的，扩大的淋巴结和CD4 T细胞活化可以观察到。这里我们详细介绍了协议隔离（从7修改）感染的肝血吸虫卵，敏感和具有挑战性的老鼠，和恢复分析机关（支气管肺泡灌洗（BAL），肺和淋巴结）。我们也有代表性的组织学和免疫学数据和其他免疫分析的建议。 总体而言，这种方法提供了在体内模型，以探讨在肺寄生虫引起的免疫反应，这是广泛适用于Th2细胞的炎症性疾病，包括寄生虫感染，纤维化疾病，过敏性炎症和哮喘的研究。这种模式的优势，为典型研究E 2的肺部发炎，包括一个强有力的Th2细胞在肺部的炎症反应和淋巴结的重复性，便于鸡蛋周围的肉芽肿病理检查炎症的评估，和潜在的长期存储的寄生虫鸡蛋。

Watch this Scientific Journal Video about Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation at JoVE.com

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

血吸虫卵是强有力的刺激T辅助2型（Th2细胞）的免疫反应，寄生虫感染的特点，哮喘和过敏性炎症。该协议利用S。血吸虫卵注射产生的CD4 Th2细胞因子诱导的炎症反应在肺，肺肉芽肿形成的特点，各地的鸡蛋，嗜酸性粒细胞和巨噬细胞替代激活。

从使用鸡蛋<em&gt;血吸虫</em&gt;作为<em&gt;在体内</em&gt;蠕虫引起的肺部炎症模型

Schistosoma parasites are blood flukes that infect an estimated 200 million people worldwide 1. In chronic infection with Schistosoma, the severe ...

Research

JoVE Journal

Immunology and Infection

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Cercarial转型和在体外栽培曼氏血吸虫 Schistosomules

Schistosome parasites are the causative agents of schistosomiasis, a chronically debilitating disease that affects over 200 million people globally and ...

科研

免疫与感染

Introducing Shear Stress in the Study of Bacterial Adhesion

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Zebrafish larvae are particularly amenable to whole animal small molecule screens1,2 due to their small size and relative ease of manipulation and ...

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

在体内成像方法区分急性和慢性炎症

Inflammation is a fundamental aspect of many human diseases. In this video report, we demonstrate non-invasive bioluminescence imaging techniques that ...

Influenza Virus Propagation in Embryonated Chicken Eggs

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Influenza infection is associated with about 36,000 deaths and more than 200,000 hospitalizations every year in the United States. The continuous ...

An Optimized Method for Isolating and Expanding Invariant Natural Killer T Cells from Mouse Spleen

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The ability to rapidly secrete cytokines upon stimulation is a functional characteristic of the invariant natural killer T (iNKT) cell lineage. iNKT cells ...

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激光内在人肺组织中的新型细菌特异探针的光学筛选

Improving the speed and accuracy of bacterial detection is important for patient stratification and to ensure the appropriate use of antimicrobials. To ...

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

人精切肺切片中物质的细胞毒性及免疫调节作用的评价

Respiratory diseases in their broad diversity need appropriate model systems to understand the underlying mechanisms and enable development of new ...

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

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成年日本血吸虫细胞外囊泡的分离与鉴定

Extracellular vesicles (EVs) are membranous vesicles released by a variety of cells into the extracellular microenvironment. EVs represent a population of ...