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54.8K Views
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07:06 min
June 29th, 2012
DOI :
10.3791/3917-v
Chapters
0:05
Title
1:04
Rat Perfusion for Liver Isolation
3:26
Hepatocyte Cell Isolation
4:42
Hepatocyte Culture
6:09
Typical Hepatocyte Harvest and 24-hour Culture Results
6:44
Conclusion
Summary
原发性肝细胞提供了一个宝贵的工具来评估生化,分子,在生理有关的实验系统和代谢功能。我们描述了可靠的协议,对大鼠原位肝灌注,一贯产生,最多to1.0×10的可行肝 8细胞与细胞之间的88〜96%的存活率。
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