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54.8K Views
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07:06 min
June 29th, 2012
DOI :
10.3791/3917-v
Chapters
0:05
Title
1:04
Rat Perfusion for Liver Isolation
3:26
Hepatocyte Cell Isolation
4:42
Hepatocyte Culture
6:09
Typical Hepatocyte Harvest and 24-hour Culture Results
6:44
Conclusion
Summary
初代肝細胞は、生理学的に関連する実験系の生化学的、分子生物学、代謝機能を評価するための貴重なツールを提供しています。我々は一貫してto1.0×10アップ可能な肝細胞を生成する現場肝灌流、ラットの信頼性の高いプロトコルを記述する 8細胞。
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