Name: use of animal model of sepsis to evaluate novel herbal therapies
Uploaded: 2012-04-11T13:00:00
Description: Watch this Scientific Journal Video about Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies at JoVE.com

이 작품은 국립 보건원, 국립 일반 의학 과학 (R01GM063075)의 연구소 보완 및 대체 의학의 국립 센터 (R01AT05076)에서 교부금에 의해 지원되었다.

1. 패혈증의 동물 모델의 확립 <ol><li> 마우스는 케타민 (75 밀리그램 / kg, 근육내, IM) 및 xylazine (10 밀리그램 / kg, IM)와 anesthetized하며, 나태한 자리에 서있는. </li><li> 안정적인 지위를 보장하기 위해 테이프로 마우스의 발을 고쳐. </li><li> betadine이나 기타 피부 소독제 및 알코올 3 교대 스크럽으로 복부를 청소합니다. 다음 15 밀리미터 중간선 절개는이게 맹장을 폭로합니다. </li><li> 대변​​의 압출 ?...

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K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anti-tumor+necrosis+factor+antibody+therapy+fails+to+prevent+lethality+after+cecal+ligation+and+puncture+or+endotoxemia.">Anti-tumor necrosis factor antibody therapy fails to prevent lethality after cecal ligation and puncture or endotoxemia.</a> J. Immunol. 148, 2724-2730 (1992).</li><li>Ziegler, E. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Treatment+of+gram-negative+bacteremia+and+septic+shock+with+HA-1A+human+monoclonal+antibody+against+endotoxin.+A+randomized,+double-blind,+placebo-controlled+trial.+The+HA-1A+Sepsis+Study+Group.">Treatment of gram-negative bacteremia and septic shock with HA-1A human monoclonal antibody against endotoxin. A randomized, double-blind, placebo-controlled trial. The HA-1A Sepsis Study Group.</a> N. Engl. J. 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TNF-alpha MAb Sepsis Study Group.</a> JAMA. 273, 934-941 (1995).</li><li>Cohen, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adjunctive+therapy+in+sepsis:+a+critical+analysis+of+the+clinical+trial+programme.">Adjunctive therapy in sepsis: a critical analysis of the clinical trial programme.</a> Br. Med. Bull. 55, 212-225 (1999).</li><li>Dellinger, R. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surviving+Sepsis+Campaign:+international+guidelines+for+management+of+severe+sepsis+and+septic+shock:+2008.">Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock: 2008.</a> Crit. Care Med. 36, 296-327 (2008).</li><li>Wang, H., Zhu, S., Zhou, R., Li, W., Sama, A. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+potential+of+HMGB1-targeting+agents+in+sepsis.">Therapeutic potential of HMGB1-targeting agents in sepsis.</a> Expert. Rev. Mol. Med. 10, e32 (2008).</li><li>Wang, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+aqueous+extract+of+a+popular+herbal+nutrient+supplement,+Angelica+sinensis,+protects+mice+against+lethal+endotoxemia+and+sepsis.">The aqueous extract of a popular herbal nutrient supplement, Angelica sinensis, protects mice against lethal endotoxemia and sepsis.</a> J. Nutr. 136, 360-365 (2006).</li><li>Li, W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+cardiovascular+drug+rescues+mice+from+lethal+sepsis+by+selectively+attenuating+a+late-acting+proinflammatory+mediator,+high+mobility+group+box+1.">A cardiovascular drug rescues mice from lethal sepsis by selectively attenuating a late-acting proinflammatory mediator, high mobility group box 1.</a> J. Immunol. 178, 3856-3864 (2007).</li><li>Fukuyama, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mixed+bacterial+infection+model+of+sepsis+in+rabbits+and+its+application+to+evaluate+superantigen-adsorbing+device.">Mixed bacterial infection model of sepsis in rabbits and its application to evaluate superantigen-adsorbing device.</a> Blood Purif. 23, 119-127 (2005).</li></ol>

실험실에서는 패혈증의 여러 동물 모델은 잠재적인 소설 요법을 개발하기 위해서는 패혈증의 pathogenesis을 이해하는 고용되었습니다. 그들의 임상적 관련 성은 패혈증에 대한 임상 응용에 대한 동물 연구의 성공적인 번역을하기 전에 논쟁의 대상이 남아있다. 조기 크린 시토킨에 대한 중화 항체 (예 : TNF)는 bacteremia / endotoxemia 17,18의 동물 모델에서 보호했지만, 그들은 실제로 패혈증 19<...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> Betadine </td><td> 퍼듀 제품 LP </td><td> 25655-41-8 </td></tr><tr><td> imipenem </td><td> 머크 &amp; 유한 주식 회사 </td><td> 9,882,821 </td></tr><tr><td> 케타민을 HCL </td><td> Hospira 주식 회사 </td><td> RL-0065 </td></tr><tr><td> Xylazine </td><td> 로이드 연구소 </td><td> 4821 </td></tr><tr><td> Autoclip </td><td> Becton 디킨슨 </td><td> 427,631 </td></tr><tr><td> 4-0 실크 봉합사 </td><td> Roboz </td><td> SUT-15-2 </td></tr><tr><td> Surflo IV 카테터 </td><td> Terumo </td><td> SR * OX2419CA </td></tr><tr><td> RayBio 마우스 시토킨 항체 어레이 </td><td> RayBiotech 주식 회사 </td><td> AAM-CYT-3 </td></tr><tr><td> Thioglycollat​​e </td><td> Becton 디킨슨 </td><td> 211,716 </td></tr></tbody></table>

use of animal model of sepsis to evaluate novel herbal therapies

패혈증은 미생물 감염으로 인한 전신 염증 반응 증후군을 말합니다. 그것은 외인성 세균 독소 (endotoxemia) 또는 박테리아 (bacteremia)의 주입뿐만 아니라 cecal 내고 주사 (CLP) 1-3 의해이게 맹장의 수술 천공 등 여러 가지 기법에 의해 동물에서 정기적으로 모의하고있다. CLP는 다공 맹장염 또는 diverticulitis의 인간 임상 질환을 흉내낸, 박테리아의 spillage 및 복막 캐비티의 배설물로 오염 수 있습니다. 같은 최종 사망률 속도에 의해 반영 패혈증의 심각도는, cecal 찔린 2 사용 바늘의 크기를 변화하여 수술 제어할 수 있습니다. 인간의 패혈증 3의 임상 과정 동안 지켜봤을 때 동물에서는 CLP는 심장 혈관 신진 대사와 면역 반응과 유사, biphasic hemodynamic을 유도합니다. 따라서 CLP 모델은 1-3 실험적인 패혈증에 가장 임상적으로 관련성이 높은 모델 중 하나로 간주됩니다. &lt;이/ P&gt; 다양한 동물 모델 실험적인 패혈증의 pathogenesis의 기본 복잡한 메커니즘을 명료하게하다하는 데 사용되었습니다. 패혈증의 치명적인 결과가 조기 크린 시토킨의 과도한 축적 4-6과 늦은 전염증성 중재자 (예 : HMGB1) 7 (예 : TNF, IL-1과 IFN-γ 등)에 부분적으로 기인 것입니다. 초기 전염증성 크린 시토킨와 비교할 때, 늦게 연기 중재자는 임상 애플 리케이션을위한 광범위한 치료 창이 있습니다. 예를 들어, CLP 후 24 시간을 시작 HMGB1 - 중화 항체의 지연 행정부는 여전히 치명적인 패혈증의 늦은 중재자로 HMGB1를 설립, 주죠 8,9에서 쥐를 구출. 늦은 연기 중재자로서 HMGB1의 발견 중국어 허벌 의학을 사용하여 패혈증 요법의 발전을위한 조사의 새로운 분야를 시작했습니다. 본 논문에서 우리는 HM에 대한 한방 의학을 심사한에서 CLP 유도된 패혈증의 절차를 설명하며 사용치료를 GB1 타겟팅.

Watch this Scientific Journal Video about Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies at JoVE.com

Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies

패혈증은 미생물 감염으로 인한 전신 염증 반응 증후군을 참조하고, cecal 내고 주사 (CLP)을 되나 수술 기법에 의한 시뮬레이션이 될 수 있습니다. 여기 치료 대리인을위한 약초를 구분할 CLP 유발 동물 모델을 사용하는 방법을 설명합니다.

소설 허벌 요법을 평가하는 패혈증의 동물 모델의 사용

Sepsis refers to a systemic inflammatory response syndrome resulting from a microbial infection. It has been routinely simulated in animals by several ...

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Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

Functional Assessment of the Donor Heart During Ex Situ Perfusion: Insights from Pressure-Volume Loops and Surface Echocardiography

현장 관류 중 기증자 심장의 기능 평가: 압력-부피 루프 및 표면 심초음파의 통찰력

Heart transplantation remains the gold standard treatment for advanced heart failure. However, the current critical organ shortage has resulted in the ...