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47.2K Views
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11:39 min
September 21st, 2012
DOI :
10.3791/3928-v
Chapters
0:05
Title
1:21
Tissue Embedding in OCT
2:13
Tissue Sectioning
2:54
Tissue Staining with Alcian Blue
3:43
Tissue Staining with Periodic Acid Schiff
4:36
Tissue Staining with Lectins
5:44
Controlling for Lectin Staining Specificity
6:52
Results: Mucin Staining in Human and Animal Tissue Sections
11:15
Conclusion
Summary
嵌入在的最优切削温度介质(OCT)的未定影的冰冻组织标本可用于研究自然分布和糖基化的分泌粘液。在这种方法中组织处理是最小的,自然呈现的糖脂,粘蛋白和糖基表位被保留。组织切片,可以用荧光或发色检测用免疫组织化学分析。
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